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Enhanced bactericidal effect of enterocin AS-48 in combination with high-intensity pulsed-electric field treatment against Salmonella enterica in apple juice.
Int J Food Microbiol. 2008 Dec 10; 128(2):244-9.IJ

Abstract

The effect of the broad spectrum cyclic antimicrobial peptide enterocin AS-48 combination with high-intensity pulsed-electric field (HIPEF) treatment (35 kV/cm, 150 Hz, 4 micros and bipolar mode) was tested on Salmonella enterica CECT 915 in apple juice. A response surface methodology was applied to study the bactericidal effects of the combined treatment. The process variables were AS-48 concentration, temperature, and HIPEF treatment time. While treatment with enterocin AS-48 alone up to 60 microg/ml had no effect on the viability of S. enterica in apple juice, an increased bactericidal activity was observed in combination with HIPEF treatments. Survival fraction was affected by treatment time, enterocin AS48 concentration and treatment temperature. The combination of 100 micros of HIPEF treatment, 30 microg/ml of AS-48, and temperature of 20 degrees C resulted in the lowest inactivation, with only a 1.2-log reduction. The maximum inactivation of 4.5-log cycles was achieved with HIPEF treatment for 1000 micros in combination with 60 microg/ml of AS-48 and a treatment temperature of 40 degrees C. Synergism between enterocin AS-48 and HIPEF treatment depended on the sequence order application, since it was observed only when HIPEF was applied in the presence of previously-added bacteriocin. The combined treatment could improve the safety of freshly-made apple juice against S. enterica transmission.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Martínez Viedma P
Area de Microbiología, Departamento de Ciencias de la Salud, Facultad de Ciencias Experimentales, Universidad de Jaén, 23071-Jaén, Spain.
Sobrino López A
No affiliation info available
Ben Omar N
No affiliation info available
Abriouel H
No affiliation info available
Lucas López R
No affiliation info available
Valdivia E
No affiliation info available
Martín Belloso O
No affiliation info available
Gálvez A
No affiliation info available

MeSH

Anti-Bacterial AgentsBacteriocinsBeveragesColony Count, MicrobialConsumer Product SafetyDrug SynergismElectromagnetic FieldsFood ContaminationFood MicrobiologyFood PreservationHumansMalusSalmonella enterica

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18829125

Citation

Martínez Viedma, Pilar, et al. "Enhanced Bactericidal Effect of Enterocin AS-48 in Combination With High-intensity Pulsed-electric Field Treatment Against Salmonella Enterica in Apple Juice." International Journal of Food Microbiology, vol. 128, no. 2, 2008, pp. 244-9.
Martínez Viedma P, Sobrino López A, Ben Omar N, et al. Enhanced bactericidal effect of enterocin AS-48 in combination with high-intensity pulsed-electric field treatment against Salmonella enterica in apple juice. Int J Food Microbiol. 2008;128(2):244-9.
Martínez Viedma, P., Sobrino López, A., Ben Omar, N., Abriouel, H., Lucas López, R., Valdivia, E., Martín Belloso, O., & Gálvez, A. (2008). Enhanced bactericidal effect of enterocin AS-48 in combination with high-intensity pulsed-electric field treatment against Salmonella enterica in apple juice. International Journal of Food Microbiology, 128(2), 244-9. https://doi.org/10.1016/j.ijfoodmicro.2008.08.014
Martínez Viedma P, et al. Enhanced Bactericidal Effect of Enterocin AS-48 in Combination With High-intensity Pulsed-electric Field Treatment Against Salmonella Enterica in Apple Juice. Int J Food Microbiol. 2008 Dec 10;128(2):244-9. PubMed PMID: 18829125.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Enhanced bactericidal effect of enterocin AS-48 in combination with high-intensity pulsed-electric field treatment against Salmonella enterica in apple juice. AU - Martínez Viedma,Pilar, AU - Sobrino López,Angel, AU - Ben Omar,Nabil, AU - Abriouel,Hikmate, AU - Lucas López,Rosario, AU - Valdivia,Eva, AU - Martín Belloso,Olga, AU - Gálvez,Antonio, Y1 - 2008/09/04/ PY - 2008/02/12/received PY - 2008/07/22/revised PY - 2008/08/24/accepted PY - 2008/10/3/pubmed PY - 2009/1/30/medline PY - 2008/10/3/entrez SP - 244 EP - 9 JF - International journal of food microbiology JO - Int J Food Microbiol VL - 128 IS - 2 N2 - The effect of the broad spectrum cyclic antimicrobial peptide enterocin AS-48 combination with high-intensity pulsed-electric field (HIPEF) treatment (35 kV/cm, 150 Hz, 4 micros and bipolar mode) was tested on Salmonella enterica CECT 915 in apple juice. A response surface methodology was applied to study the bactericidal effects of the combined treatment. The process variables were AS-48 concentration, temperature, and HIPEF treatment time. While treatment with enterocin AS-48 alone up to 60 microg/ml had no effect on the viability of S. enterica in apple juice, an increased bactericidal activity was observed in combination with HIPEF treatments. Survival fraction was affected by treatment time, enterocin AS48 concentration and treatment temperature. The combination of 100 micros of HIPEF treatment, 30 microg/ml of AS-48, and temperature of 20 degrees C resulted in the lowest inactivation, with only a 1.2-log reduction. The maximum inactivation of 4.5-log cycles was achieved with HIPEF treatment for 1000 micros in combination with 60 microg/ml of AS-48 and a treatment temperature of 40 degrees C. Synergism between enterocin AS-48 and HIPEF treatment depended on the sequence order application, since it was observed only when HIPEF was applied in the presence of previously-added bacteriocin. The combined treatment could improve the safety of freshly-made apple juice against S. enterica transmission. SN - 0168-1605 UR - https://www.unboundmedicine.com/medline/citation/18829125/Enhanced_bactericidal_effect_of_enterocin_AS_48_in_combination_with_high_intensity_pulsed_electric_field_treatment_against_Salmonella_enterica_in_apple_juice_ DB - PRIME DP - Unbound Medicine ER -