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The gastrointestinal microbiota affects the selenium status and selenoprotein expression in mice.
J Nutr Biochem 2009; 20(8):638-48JN

Abstract

Colonization of germ-free (GF) mice has been shown to induce the gastrointestinal form of the selenium-dependent glutathione peroxidases, GPx2. Since bacterial colonization of the gastrointestinal tract is associated with stress, we aimed to clarify how bacteria affect selenoprotein expression in unstressed conditions. GF and conventional (CV) FVB/NHan(TMHsd) mice were fed a selenium-poor (0.086 ppm) or a selenium-adequate (0.15 ppm) diet for 5 weeks starting from weaning. Each group consisted of five animals. Specific glutathione peroxidase (GPx) and thioredoxin reductase (TrxR) expression was measured in plasma, liver and intestinal sections by activity, protein and mRNA level as appropriate. Under selenium-adequate conditions, selenoprotein expression did not differ in GF and CV mice. Under selenium-limiting conditions, however, GF mice generally contained higher GPx and TrxR activities in the intestine and liver, higher GPx1 protein and RNA levels in the liver, higher GPx2 protein levels in the proximal and distal jejunum and colon and higher GPx1 and GPx2 RNA levels in the colon. In addition, higher selenium concentrations were estimated in plasma, liver and cecum. All differences were significant. It is concluded that bacteria may compete with the host for selenium when availability becomes limiting. A variable association with different microorganisms might influence the daily requirement of mice for selenium. Whether the microbiota also affects the human selenoprotein status appears worthy of investigation.

Authors+Show Affiliations

Department of Biochemistry of Micronutrients, German Institute of Human Nutrition Potsdam-Rehbruecke, Nuthetal, Germany.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18829286

Citation

Hrdina, Juliane, et al. "The Gastrointestinal Microbiota Affects the Selenium Status and Selenoprotein Expression in Mice." The Journal of Nutritional Biochemistry, vol. 20, no. 8, 2009, pp. 638-48.
Hrdina J, Banning A, Kipp A, et al. The gastrointestinal microbiota affects the selenium status and selenoprotein expression in mice. J Nutr Biochem. 2009;20(8):638-48.
Hrdina, J., Banning, A., Kipp, A., Loh, G., Blaut, M., & Brigelius-Flohé, R. (2009). The gastrointestinal microbiota affects the selenium status and selenoprotein expression in mice. The Journal of Nutritional Biochemistry, 20(8), pp. 638-48. doi:10.1016/j.jnutbio.2008.06.009.
Hrdina J, et al. The Gastrointestinal Microbiota Affects the Selenium Status and Selenoprotein Expression in Mice. J Nutr Biochem. 2009;20(8):638-48. PubMed PMID: 18829286.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The gastrointestinal microbiota affects the selenium status and selenoprotein expression in mice. AU - Hrdina,Juliane, AU - Banning,Antje, AU - Kipp,Anna, AU - Loh,Gunnar, AU - Blaut,Michael, AU - Brigelius-Flohé,Regina, Y1 - 2008/09/30/ PY - 2008/02/20/received PY - 2008/06/12/revised PY - 2008/06/17/accepted PY - 2008/10/3/pubmed PY - 2009/8/14/medline PY - 2008/10/3/entrez SP - 638 EP - 48 JF - The Journal of nutritional biochemistry JO - J. Nutr. Biochem. VL - 20 IS - 8 N2 - Colonization of germ-free (GF) mice has been shown to induce the gastrointestinal form of the selenium-dependent glutathione peroxidases, GPx2. Since bacterial colonization of the gastrointestinal tract is associated with stress, we aimed to clarify how bacteria affect selenoprotein expression in unstressed conditions. GF and conventional (CV) FVB/NHan(TMHsd) mice were fed a selenium-poor (0.086 ppm) or a selenium-adequate (0.15 ppm) diet for 5 weeks starting from weaning. Each group consisted of five animals. Specific glutathione peroxidase (GPx) and thioredoxin reductase (TrxR) expression was measured in plasma, liver and intestinal sections by activity, protein and mRNA level as appropriate. Under selenium-adequate conditions, selenoprotein expression did not differ in GF and CV mice. Under selenium-limiting conditions, however, GF mice generally contained higher GPx and TrxR activities in the intestine and liver, higher GPx1 protein and RNA levels in the liver, higher GPx2 protein levels in the proximal and distal jejunum and colon and higher GPx1 and GPx2 RNA levels in the colon. In addition, higher selenium concentrations were estimated in plasma, liver and cecum. All differences were significant. It is concluded that bacteria may compete with the host for selenium when availability becomes limiting. A variable association with different microorganisms might influence the daily requirement of mice for selenium. Whether the microbiota also affects the human selenoprotein status appears worthy of investigation. SN - 1873-4847 UR - https://www.unboundmedicine.com/medline/citation/18829286/The_gastrointestinal_microbiota_affects_the_selenium_status_and_selenoprotein_expression_in_mice_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0955-2863(08)00146-0 DB - PRIME DP - Unbound Medicine ER -