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Combination of aptamer with gold nanoparticles for electrochemical signal amplification: application to sensitive detection of platelet-derived growth factor.
Biosens Bioelectron. 2009 Feb 15; 24(6):1598-602.BB

Abstract

In this paper, we report a novel electrochemical detection approach for platelet-derived growth factor (PDGF) via "sandwich" structure and gold nanoparticles (Au-NPs) mediated amplification technique. The "sandwich" structure is fabricated based on the fact that PDGF has two aptamer-binding sites, which makes it possible for one PDGF molecule to connect with two aptamers simultaneously. It is found that this electrochemical system with "sandwich" structure and Au-NPs can significantly amplify the signal of electrochemical probe [Ru(NH(3))(5)Cl](2+) for PDGF detection, and thus increase the detection sensitivity significantly. As a result, this PDGF detection approach obtains an extraordinarily low detection limit of 1 x 10(-14)M for purified samples, 1 x 10(-12)M for contaminated-ridden samples or undiluted blood serum. This detection approach can also exhibit good stability and excellent specificity.

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Authors+Show Affiliations

Wang J
Department of Biochemistry and National Key Laboratory of Pharmaceutical Biotechnology, Nanjing University, Nanjing, China.
Meng W
No affiliation info available
Zheng X
No affiliation info available
Liu S
No affiliation info available
Li G
No affiliation info available

MeSH

Aptamers, NucleotideBiosensing TechniquesElectrochemistryEquipment DesignEquipment Failure AnalysisGoldMicrochemistryNanoparticlesNanotechnologyPlatelet-Derived Growth FactorReproducibility of ResultsSensitivity and Specificity

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18829294

Citation

Wang, Jing, et al. "Combination of Aptamer With Gold Nanoparticles for Electrochemical Signal Amplification: Application to Sensitive Detection of Platelet-derived Growth Factor." Biosensors & Bioelectronics, vol. 24, no. 6, 2009, pp. 1598-602.
Wang J, Meng W, Zheng X, et al. Combination of aptamer with gold nanoparticles for electrochemical signal amplification: application to sensitive detection of platelet-derived growth factor. Biosens Bioelectron. 2009;24(6):1598-602.
Wang, J., Meng, W., Zheng, X., Liu, S., & Li, G. (2009). Combination of aptamer with gold nanoparticles for electrochemical signal amplification: application to sensitive detection of platelet-derived growth factor. Biosensors & Bioelectronics, 24(6), 1598-602. https://doi.org/10.1016/j.bios.2008.08.030
Wang J, et al. Combination of Aptamer With Gold Nanoparticles for Electrochemical Signal Amplification: Application to Sensitive Detection of Platelet-derived Growth Factor. Biosens Bioelectron. 2009 Feb 15;24(6):1598-602. PubMed PMID: 18829294.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Combination of aptamer with gold nanoparticles for electrochemical signal amplification: application to sensitive detection of platelet-derived growth factor. AU - Wang,Jing, AU - Meng,Wenying, AU - Zheng,Xiaofeng, AU - Liu,Shanli, AU - Li,Genxi, Y1 - 2008/08/27/ PY - 2008/05/19/received PY - 2008/07/17/revised PY - 2008/08/11/accepted PY - 2008/10/3/pubmed PY - 2009/5/27/medline PY - 2008/10/3/entrez SP - 1598 EP - 602 JF - Biosensors & bioelectronics JO - Biosens Bioelectron VL - 24 IS - 6 N2 - In this paper, we report a novel electrochemical detection approach for platelet-derived growth factor (PDGF) via "sandwich" structure and gold nanoparticles (Au-NPs) mediated amplification technique. The "sandwich" structure is fabricated based on the fact that PDGF has two aptamer-binding sites, which makes it possible for one PDGF molecule to connect with two aptamers simultaneously. It is found that this electrochemical system with "sandwich" structure and Au-NPs can significantly amplify the signal of electrochemical probe [Ru(NH(3))(5)Cl](2+) for PDGF detection, and thus increase the detection sensitivity significantly. As a result, this PDGF detection approach obtains an extraordinarily low detection limit of 1 x 10(-14)M for purified samples, 1 x 10(-12)M for contaminated-ridden samples or undiluted blood serum. This detection approach can also exhibit good stability and excellent specificity. SN - 1873-4235 UR - https://www.unboundmedicine.com/medline/citation/18829294/Combination_of_aptamer_with_gold_nanoparticles_for_electrochemical_signal_amplification:_application_to_sensitive_detection_of_platelet_derived_growth_factor_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0956-5663(08)00484-3 DB - PRIME DP - Unbound Medicine ER -