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Confirmation of BD ProbeTec Neisseria gonorrhoeae reactive samples by Gen-Probe APTIMA assays and culture.
Sex Transm Infect. 2009 Feb; 85(1):24-6.ST

Abstract

BACKGROUND

Use of nucleic acid amplification tests (NAATs), such as strand displacement assay (SDA), for the detection of gonococcal infection in low prevalence populations is controversial because of the likelihood of false positive results. Use of supplementary NAATs with alternative target sites has been recommended for confirmation of primary NAAT results.

AIM

To evaluate if SDA reactive specimens for Neisseria gonorrhoeae, which were either culture positive or negative, can be confirmed by alternative target NAATs such as transcription-mediated assays (TMA).

METHODS

SDA reactive specimens were retested by TMA using APTIMA Combo 2 (AC2) and APTIMA GC (AGC) assays. Two different methods of specimen preparation were used to test the specimens. In method A, residual extract after SDA was retested and in method B, the original clinical specimen was re-extracted in TMA medium and then retested. Cervical or urethral swabs were requested to confirm the SDA results by culture.

RESULTS

By method A, 26/49 (53.1%) of SDA positive specimens were positive by AC2 and/or AGC; 14/27 (51.8%) culture confirmed SDA positive tests were positive by AC2 and/or AGC. By method B, 38/39 (97.3%) SDA positive results were confirmed by both AC2 and AGC. All the 25 culture confirmed SDA positive tests were confirmed by both AC2 and AGC; 5/6 SDA positive tests that were culture negative were confirmed by both AC2 and/AGC.

CONCLUSION

Alternative target site NAATs, such as AC2 and AGC, can be used to confirm SDA positive results using the same clinical specimen. There is high concordance between the three NAATs.

Authors+Show Affiliations

Department of Clinical Microbiology, University Hospital Lewisham, London, UK.No affiliation info availableNo affiliation info available

Pub Type(s)

Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18829626

Citation

Hardwick, R, et al. "Confirmation of BD ProbeTec Neisseria Gonorrhoeae Reactive Samples By Gen-Probe APTIMA Assays and Culture." Sexually Transmitted Infections, vol. 85, no. 1, 2009, pp. 24-6.
Hardwick R, Gopal Rao G, Mallinson H. Confirmation of BD ProbeTec Neisseria gonorrhoeae reactive samples by Gen-Probe APTIMA assays and culture. Sex Transm Infect. 2009;85(1):24-6.
Hardwick, R., Gopal Rao, G., & Mallinson, H. (2009). Confirmation of BD ProbeTec Neisseria gonorrhoeae reactive samples by Gen-Probe APTIMA assays and culture. Sexually Transmitted Infections, 85(1), 24-6. https://doi.org/10.1136/sti.2008.032789
Hardwick R, Gopal Rao G, Mallinson H. Confirmation of BD ProbeTec Neisseria Gonorrhoeae Reactive Samples By Gen-Probe APTIMA Assays and Culture. Sex Transm Infect. 2009;85(1):24-6. PubMed PMID: 18829626.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Confirmation of BD ProbeTec Neisseria gonorrhoeae reactive samples by Gen-Probe APTIMA assays and culture. AU - Hardwick,R, AU - Gopal Rao,G, AU - Mallinson,H, Y1 - 2008/10/01/ PY - 2008/10/3/pubmed PY - 2009/4/9/medline PY - 2008/10/3/entrez SP - 24 EP - 6 JF - Sexually transmitted infections JO - Sex Transm Infect VL - 85 IS - 1 N2 - BACKGROUND: Use of nucleic acid amplification tests (NAATs), such as strand displacement assay (SDA), for the detection of gonococcal infection in low prevalence populations is controversial because of the likelihood of false positive results. Use of supplementary NAATs with alternative target sites has been recommended for confirmation of primary NAAT results. AIM: To evaluate if SDA reactive specimens for Neisseria gonorrhoeae, which were either culture positive or negative, can be confirmed by alternative target NAATs such as transcription-mediated assays (TMA). METHODS: SDA reactive specimens were retested by TMA using APTIMA Combo 2 (AC2) and APTIMA GC (AGC) assays. Two different methods of specimen preparation were used to test the specimens. In method A, residual extract after SDA was retested and in method B, the original clinical specimen was re-extracted in TMA medium and then retested. Cervical or urethral swabs were requested to confirm the SDA results by culture. RESULTS: By method A, 26/49 (53.1%) of SDA positive specimens were positive by AC2 and/or AGC; 14/27 (51.8%) culture confirmed SDA positive tests were positive by AC2 and/or AGC. By method B, 38/39 (97.3%) SDA positive results were confirmed by both AC2 and AGC. All the 25 culture confirmed SDA positive tests were confirmed by both AC2 and AGC; 5/6 SDA positive tests that were culture negative were confirmed by both AC2 and/AGC. CONCLUSION: Alternative target site NAATs, such as AC2 and AGC, can be used to confirm SDA positive results using the same clinical specimen. There is high concordance between the three NAATs. SN - 1472-3263 UR - https://www.unboundmedicine.com/medline/citation/18829626/Confirmation_of_BD_ProbeTec_Neisseria_gonorrhoeae_reactive_samples_by_Gen_Probe_APTIMA_assays_and_culture_ L2 - https://sti.bmj.com/lookup/pmidlookup?view=long&pmid=18829626 DB - PRIME DP - Unbound Medicine ER -