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Alterations in CD4+, CD8+, Vgamma3, Vgammadelta, and/or Valpha betaT-lymphocyte expression in lymphoid tissues of progeny after in utero exposure to benzo(alpha)pyrene.
J Immunotoxicol. 2008 Jul; 5(3):293-306.JI

Abstract

That benzo alpha)pyrene (Balpha P) decreases both humoral and cell-mediated immunity, and leads to increases in progeny tumor development after in utero insult, suggests that T- and B-lymphocytes are made defective in exposed offspring. In the study here, C3H mice were injected once with Balpha P (150 microg/g BW) at day 12 of pregnancy and progeny lymphoid tissues were excised during gestation (day 18; GD18) or at 1 or 6 weeks post-partum. The isolated lymphoid cells were analyzed by flow cytometry/immunofluorescence or assessed for function. In Balpha P-exposed fetuses, thymic Thy1(+) cell levels were decreased (relative to levels in organs of corn oil-exposed dam progeny). In addition, for up to 6 weeks post-birth, CD4(+)CD8(+) (double positive; DP) cells were virtually absent and levels of CD4(-)CD8(-) (double negative; DN) cells were consistently at epsilon 90%. With regard to single positive (SP) cells, CD4(+) cell levels were also decreased in tissues at GD18 up through 6 weeks post-birth; CD8(+) cell levels were increased, but only in pups at 1-week and 6-weeks post-birth. In 1-week-old progeny, spleen CD8(+) cell levels were quantitatively unchanged, though CD4(+) levels were reduced 2-4-fold and CD4(-)CD8(-) DN levels significantly increased. With respect to TCRs, fetal levels of thymic CD3Vgamma(3)(+) and CD3Vgamma delta(+) cells were decreased; levels of CD3Valphabeta cells were only slightly depressed. The latter results contrast sharply with a strong reduction in CD3Valphabeta cells in the fetal livers of Balpha P-exposed progeny. Interestingly, these livers also strongly evidenced a presence of BalphaP-7,8-dihydrodiol-9,10-epoxide metabolite. When assessed for any change in function, the CD4(+), Thy1(+) cells isolated from Balpha P-exposed progeny tissues responded weakly (relative to controls) to ConA and in an allogeneic MLR. Taken in totality, the results here strengthen our original hypothesis that BalphaP can create a favorable milieu for tumor growth progression in progeny of exposed mothers by affecting development of sufficient numbers of functional lymphocytes in the offspring.

Authors+Show Affiliations

Department of Microbiology and Immunology, The Morehouse School of Medicine, Atlanta, GA 30310, USA. paul-urso@bellsouth.netNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, N.I.H., Extramural
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

18830890

Citation

Urso, P, et al. "Alterations in CD4+, CD8+, Vgamma3, Vgammadelta, And/or Valpha betaT-lymphocyte Expression in Lymphoid Tissues of Progeny After in Utero Exposure to Benzo(alpha)pyrene." Journal of Immunotoxicology, vol. 5, no. 3, 2008, pp. 293-306.
Urso P, Wirsiy YG, Zhang W, et al. Alterations in CD4+, CD8+, Vgamma3, Vgammadelta, and/or Valpha betaT-lymphocyte expression in lymphoid tissues of progeny after in utero exposure to benzo(alpha)pyrene. J Immunotoxicol. 2008;5(3):293-306.
Urso, P., Wirsiy, Y. G., Zhang, W., & Moolenaar-Wirsiy, P. J. (2008). Alterations in CD4+, CD8+, Vgamma3, Vgammadelta, and/or Valpha betaT-lymphocyte expression in lymphoid tissues of progeny after in utero exposure to benzo(alpha)pyrene. Journal of Immunotoxicology, 5(3), 293-306. https://doi.org/10.1080/15376510802312324
Urso P, et al. Alterations in CD4+, CD8+, Vgamma3, Vgammadelta, And/or Valpha betaT-lymphocyte Expression in Lymphoid Tissues of Progeny After in Utero Exposure to Benzo(alpha)pyrene. J Immunotoxicol. 2008;5(3):293-306. PubMed PMID: 18830890.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Alterations in CD4+, CD8+, Vgamma3, Vgammadelta, and/or Valpha betaT-lymphocyte expression in lymphoid tissues of progeny after in utero exposure to benzo(alpha)pyrene. AU - Urso,P, AU - Wirsiy,Y G, AU - Zhang,W, AU - Moolenaar-Wirsiy,P J, PY - 2008/10/3/pubmed PY - 2008/12/23/medline PY - 2008/10/3/entrez SP - 293 EP - 306 JF - Journal of immunotoxicology JO - J Immunotoxicol VL - 5 IS - 3 N2 - That benzo alpha)pyrene (Balpha P) decreases both humoral and cell-mediated immunity, and leads to increases in progeny tumor development after in utero insult, suggests that T- and B-lymphocytes are made defective in exposed offspring. In the study here, C3H mice were injected once with Balpha P (150 microg/g BW) at day 12 of pregnancy and progeny lymphoid tissues were excised during gestation (day 18; GD18) or at 1 or 6 weeks post-partum. The isolated lymphoid cells were analyzed by flow cytometry/immunofluorescence or assessed for function. In Balpha P-exposed fetuses, thymic Thy1(+) cell levels were decreased (relative to levels in organs of corn oil-exposed dam progeny). In addition, for up to 6 weeks post-birth, CD4(+)CD8(+) (double positive; DP) cells were virtually absent and levels of CD4(-)CD8(-) (double negative; DN) cells were consistently at epsilon 90%. With regard to single positive (SP) cells, CD4(+) cell levels were also decreased in tissues at GD18 up through 6 weeks post-birth; CD8(+) cell levels were increased, but only in pups at 1-week and 6-weeks post-birth. In 1-week-old progeny, spleen CD8(+) cell levels were quantitatively unchanged, though CD4(+) levels were reduced 2-4-fold and CD4(-)CD8(-) DN levels significantly increased. With respect to TCRs, fetal levels of thymic CD3Vgamma(3)(+) and CD3Vgamma delta(+) cells were decreased; levels of CD3Valphabeta cells were only slightly depressed. The latter results contrast sharply with a strong reduction in CD3Valphabeta cells in the fetal livers of Balpha P-exposed progeny. Interestingly, these livers also strongly evidenced a presence of BalphaP-7,8-dihydrodiol-9,10-epoxide metabolite. When assessed for any change in function, the CD4(+), Thy1(+) cells isolated from Balpha P-exposed progeny tissues responded weakly (relative to controls) to ConA and in an allogeneic MLR. Taken in totality, the results here strengthen our original hypothesis that BalphaP can create a favorable milieu for tumor growth progression in progeny of exposed mothers by affecting development of sufficient numbers of functional lymphocytes in the offspring. SN - 1547-6901 UR - https://www.unboundmedicine.com/medline/citation/18830890/Alterations_in_CD4+_CD8+_Vgamma3_Vgammadelta_and/or_Valpha_betaT_lymphocyte_expression_in_lymphoid_tissues_of_progeny_after_in_utero_exposure_to_benzo_alpha_pyrene_ L2 - https://www.tandfonline.com/doi/full/10.1080/15376510802312324 DB - PRIME DP - Unbound Medicine ER -