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Comparison of plaque reduction neutralisation test (PRNT) and measles virus-specific IgG ELISA for assessing immunogenicity of measles vaccination.
Vaccine. 2008 Nov 25; 26(50):6392-7.V

Abstract

BACKGROUND

Plaque reduction neutralisation test (PRNT) is a gold standard assay for measles antibodies. PRNT, however, is time-consuming and more difficult to standardise than ELISA.

METHODS

PRNT results were obtained on pre- and post-vaccination sera from 501 measles-vaccinated infants. From this cohort a sub-set of 210 infants were selected for testing by ELISA, which was performed either manually or by an automated processor. Results were corrected for sampling proportion before comparing the assays.

RESULTS

Seroconversion was detected by PRNT in 454 infants of whom 324 were estimated to have seroconverted by manual ELISA and 191 by automated ELISA It is recommended that ELISA negative post-vaccination samples are re-tested by PRNT to detect all seroconversions.

Links

Publisher Full Text (DOI)

Authors+Show Affiliations

Cohen BJ
Virus Reference Department, Centre for Infections, Health Protection Agency, 61 Colindale Avenue, London NW9 5EQ, United Kingdom. Bernard.Cohen@hpa.org.uk
Doblas D
No affiliation info available
Andrews N
No affiliation info available

MeSH

Antibodies, ViralAntibody SpecificityEnzyme-Linked Immunosorbent AssayHumansImmunoglobulin GInfantMeaslesMeasles VaccineMeasles virusNeutralization TestsPredictive Value of TestsSensitivity and SpecificityVaccinationViral Plaque Assay

Pub Type(s)

Comparative Study
Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18834911

Citation

Cohen, B J., et al. "Comparison of Plaque Reduction Neutralisation Test (PRNT) and Measles Virus-specific IgG ELISA for Assessing Immunogenicity of Measles Vaccination." Vaccine, vol. 26, no. 50, 2008, pp. 6392-7.
Cohen BJ, Doblas D, Andrews N. Comparison of plaque reduction neutralisation test (PRNT) and measles virus-specific IgG ELISA for assessing immunogenicity of measles vaccination. Vaccine. 2008;26(50):6392-7.
Cohen, B. J., Doblas, D., & Andrews, N. (2008). Comparison of plaque reduction neutralisation test (PRNT) and measles virus-specific IgG ELISA for assessing immunogenicity of measles vaccination. Vaccine, 26(50), 6392-7. https://doi.org/10.1016/j.vaccine.2008.08.074
Cohen BJ, Doblas D, Andrews N. Comparison of Plaque Reduction Neutralisation Test (PRNT) and Measles Virus-specific IgG ELISA for Assessing Immunogenicity of Measles Vaccination. Vaccine. 2008 Nov 25;26(50):6392-7. PubMed PMID: 18834911.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Comparison of plaque reduction neutralisation test (PRNT) and measles virus-specific IgG ELISA for assessing immunogenicity of measles vaccination. AU - Cohen,B J, AU - Doblas,D, AU - Andrews,N, Y1 - 2008/10/01/ PY - 2008/06/27/received PY - 2008/07/30/revised PY - 2008/08/31/accepted PY - 2008/10/7/pubmed PY - 2009/2/10/medline PY - 2008/10/7/entrez SP - 6392 EP - 7 JF - Vaccine JO - Vaccine VL - 26 IS - 50 N2 - BACKGROUND: Plaque reduction neutralisation test (PRNT) is a gold standard assay for measles antibodies. PRNT, however, is time-consuming and more difficult to standardise than ELISA. METHODS: PRNT results were obtained on pre- and post-vaccination sera from 501 measles-vaccinated infants. From this cohort a sub-set of 210 infants were selected for testing by ELISA, which was performed either manually or by an automated processor. Results were corrected for sampling proportion before comparing the assays. RESULTS: Seroconversion was detected by PRNT in 454 infants of whom 324 were estimated to have seroconverted by manual ELISA and 191 by automated ELISA It is recommended that ELISA negative post-vaccination samples are re-tested by PRNT to detect all seroconversions. SN - 0264-410X UR - https://www.unboundmedicine.com/medline/citation/18834911/Comparison_of_plaque_reduction_neutralisation_test__PRNT__and_measles_virus_specific_IgG_ELISA_for_assessing_immunogenicity_of_measles_vaccination_ DB - PRIME DP - Unbound Medicine ER -