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Separate hydrolysis and fermentation (SHF) of Prosopis juliflora, a woody substrate, for the production of cellulosic ethanol by Saccharomyces cerevisiae and Pichia stipitis-NCIM 3498.
Bioresour Technol. 2009 Feb; 100(3):1214-20.BT

Abstract

Prosopis juliflora (Mesquite) is a raw material for long-term sustainable production of cellulosics ethanol. In this study, we used acid pretreatment, delignification and enzymatic hydrolysis to evaluate the pretreatment to produce more sugar, to be fermented to ethanol. Dilute H(2)SO(4) (3.0%,v/v) treatment resulted in hydrolysis of hemicelluloses from lignocellulosic complex to pentose sugars along with other byproducts such as furfural, hydroxymethyl furfural (HMF), phenolics and acetic acid. The acid pretreated substrate was delignified to the extent of 93.2% by the combined action of sodium sulphite (5.0%,w/v) and sodium chlorite (3.0%,w/v). The remaining cellulosic residue was enzymatically hydrolyzed in 0.05 M citrate phosphate buffer (pH 5.0) using 3.0 U of filter paper cellulase (FPase) and 9.0 U of beta-glucosidase per mL of citrate phosphate buffer. The maximum enzymatic saccharification of cellulosic material (82.8%) was achieved after 28 h incubation at 50 degrees C. The fermentation of both acid and enzymatic hydrolysates, containing 18.24 g/L and 37.47 g/L sugars, with Pichia stipitis and Saccharomyces cerevisiae produced 7.13 g/L and 18.52 g/L of ethanol with corresponding yield of 0.39 g/g and 0.49 g/g, respectively.

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Authors+Show Affiliations

Gupta R
Department of Microbiology, Lignocellulose Biotechnology Laboratory, University of Delhi South Campus, New Delhi, India.
Sharma KK
No affiliation info available
Kuhad RC
No affiliation info available

MeSH

CelluloseEthanolFermentationHydrolysisPichiaProsopisSaccharomyces cerevisiaeWood

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18835157

Citation

Gupta, Rishi, et al. "Separate Hydrolysis and Fermentation (SHF) of Prosopis Juliflora, a Woody Substrate, for the Production of Cellulosic Ethanol By Saccharomyces Cerevisiae and Pichia stipitis-NCIM 3498." Bioresource Technology, vol. 100, no. 3, 2009, pp. 1214-20.
Gupta R, Sharma KK, Kuhad RC. Separate hydrolysis and fermentation (SHF) of Prosopis juliflora, a woody substrate, for the production of cellulosic ethanol by Saccharomyces cerevisiae and Pichia stipitis-NCIM 3498. Bioresour Technol. 2009;100(3):1214-20.
Gupta, R., Sharma, K. K., & Kuhad, R. C. (2009). Separate hydrolysis and fermentation (SHF) of Prosopis juliflora, a woody substrate, for the production of cellulosic ethanol by Saccharomyces cerevisiae and Pichia stipitis-NCIM 3498. Bioresource Technology, 100(3), 1214-20. https://doi.org/10.1016/j.biortech.2008.08.033
Gupta R, Sharma KK, Kuhad RC. Separate Hydrolysis and Fermentation (SHF) of Prosopis Juliflora, a Woody Substrate, for the Production of Cellulosic Ethanol By Saccharomyces Cerevisiae and Pichia stipitis-NCIM 3498. Bioresour Technol. 2009;100(3):1214-20. PubMed PMID: 18835157.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Separate hydrolysis and fermentation (SHF) of Prosopis juliflora, a woody substrate, for the production of cellulosic ethanol by Saccharomyces cerevisiae and Pichia stipitis-NCIM 3498. AU - Gupta,Rishi, AU - Sharma,Krishna Kant, AU - Kuhad,Ramesh Chander, Y1 - 2008/10/02/ PY - 2008/04/19/received PY - 2008/08/20/revised PY - 2008/08/20/accepted PY - 2008/10/7/pubmed PY - 2008/12/30/medline PY - 2008/10/7/entrez SP - 1214 EP - 20 JF - Bioresource technology JO - Bioresour Technol VL - 100 IS - 3 N2 - Prosopis juliflora (Mesquite) is a raw material for long-term sustainable production of cellulosics ethanol. In this study, we used acid pretreatment, delignification and enzymatic hydrolysis to evaluate the pretreatment to produce more sugar, to be fermented to ethanol. Dilute H(2)SO(4) (3.0%,v/v) treatment resulted in hydrolysis of hemicelluloses from lignocellulosic complex to pentose sugars along with other byproducts such as furfural, hydroxymethyl furfural (HMF), phenolics and acetic acid. The acid pretreated substrate was delignified to the extent of 93.2% by the combined action of sodium sulphite (5.0%,w/v) and sodium chlorite (3.0%,w/v). The remaining cellulosic residue was enzymatically hydrolyzed in 0.05 M citrate phosphate buffer (pH 5.0) using 3.0 U of filter paper cellulase (FPase) and 9.0 U of beta-glucosidase per mL of citrate phosphate buffer. The maximum enzymatic saccharification of cellulosic material (82.8%) was achieved after 28 h incubation at 50 degrees C. The fermentation of both acid and enzymatic hydrolysates, containing 18.24 g/L and 37.47 g/L sugars, with Pichia stipitis and Saccharomyces cerevisiae produced 7.13 g/L and 18.52 g/L of ethanol with corresponding yield of 0.39 g/g and 0.49 g/g, respectively. SN - 1873-2976 UR - https://www.unboundmedicine.com/medline/citation/18835157/Separate_hydrolysis_and_fermentation__SHF__of_Prosopis_juliflora_a_woody_substrate_for_the_production_of_cellulosic_ethanol_by_Saccharomyces_cerevisiae_and_Pichia_stipitis_NCIM_3498_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0960-8524(08)00730-X DB - PRIME DP - Unbound Medicine ER -