Tags

Type your tag names separated by a space and hit enter

Fermentative capacity of dry active wine yeast requires a specific oxidative stress response during industrial biomass growth.
Appl Microbiol Biotechnol. 2009 Jan; 81(5):951-60.AM

Abstract

Induction of the oxidative stress response has been described under many physiological conditions in Saccharomyces cerevisiae, including industrial fermentation for wine yeast biomass production where cells are grown through several batch and fed-batch cultures on molasses. Here, we investigate the influence of aeration on the expression changes of different gene markers for oxidative stress and compare the induction profiles to the accumulation of several intracellular metabolites in order to correlate the molecular response to physiological and metabolic changes. We also demonstrate that this specific oxidative response is relevant for wine yeast performance by construction of a genetically engineered wine yeast strain overexpressing the TRX2 gene that codifies a thioredoxin, one of the most important cellular defenses against oxidative damage. This modified strain displays an improved fermentative capacity and lower levels of oxidative cellular damages than its parental strain after dry biomass production.

Links

Aggregator Full Text

Authors+Show Affiliations

Pérez-Torrado R
Departamento de Biotecnología, Instituto de Agroquímica y Tecnología de Alimentos, CSIC, Apartado de Correos, 73. Burjassot, Valencia 46100, Spain.
Gómez-Pastor R
No affiliation info available
Larsson C
No affiliation info available
Matallana E
No affiliation info available

MeSH

AirBiomassFermentationGene DosageGene Expression ProfilingMetabolomeOxidative StressOxygenSaccharomyces cerevisiaeSaccharomyces cerevisiae ProteinsSucroseThioredoxinsWine

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18836715

Citation

Pérez-Torrado, Roberto, et al. "Fermentative Capacity of Dry Active Wine Yeast Requires a Specific Oxidative Stress Response During Industrial Biomass Growth." Applied Microbiology and Biotechnology, vol. 81, no. 5, 2009, pp. 951-60.
Pérez-Torrado R, Gómez-Pastor R, Larsson C, et al. Fermentative capacity of dry active wine yeast requires a specific oxidative stress response during industrial biomass growth. Appl Microbiol Biotechnol. 2009;81(5):951-60.
Pérez-Torrado, R., Gómez-Pastor, R., Larsson, C., & Matallana, E. (2009). Fermentative capacity of dry active wine yeast requires a specific oxidative stress response during industrial biomass growth. Applied Microbiology and Biotechnology, 81(5), 951-60. https://doi.org/10.1007/s00253-008-1722-9
Pérez-Torrado R, et al. Fermentative Capacity of Dry Active Wine Yeast Requires a Specific Oxidative Stress Response During Industrial Biomass Growth. Appl Microbiol Biotechnol. 2009;81(5):951-60. PubMed PMID: 18836715.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Fermentative capacity of dry active wine yeast requires a specific oxidative stress response during industrial biomass growth. AU - Pérez-Torrado,Roberto, AU - Gómez-Pastor,Rocío, AU - Larsson,Christer, AU - Matallana,Emilia, Y1 - 2008/10/03/ PY - 2008/07/28/received PY - 2008/09/16/accepted PY - 2008/09/16/revised PY - 2008/10/7/pubmed PY - 2009/1/17/medline PY - 2008/10/7/entrez SP - 951 EP - 60 JF - Applied microbiology and biotechnology JO - Appl Microbiol Biotechnol VL - 81 IS - 5 N2 - Induction of the oxidative stress response has been described under many physiological conditions in Saccharomyces cerevisiae, including industrial fermentation for wine yeast biomass production where cells are grown through several batch and fed-batch cultures on molasses. Here, we investigate the influence of aeration on the expression changes of different gene markers for oxidative stress and compare the induction profiles to the accumulation of several intracellular metabolites in order to correlate the molecular response to physiological and metabolic changes. We also demonstrate that this specific oxidative response is relevant for wine yeast performance by construction of a genetically engineered wine yeast strain overexpressing the TRX2 gene that codifies a thioredoxin, one of the most important cellular defenses against oxidative damage. This modified strain displays an improved fermentative capacity and lower levels of oxidative cellular damages than its parental strain after dry biomass production. SN - 1432-0614 UR - https://www.unboundmedicine.com/medline/citation/18836715/Fermentative_capacity_of_dry_active_wine_yeast_requires_a_specific_oxidative_stress_response_during_industrial_biomass_growth_ L2 - https://dx.doi.org/10.1007/s00253-008-1722-9 DB - PRIME DP - Unbound Medicine ER -