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ATP induces long-term potentiation of C-fiber-evoked field potentials in spinal dorsal horn: the roles of P2X4 receptors and p38 MAPK in microglia.
Glia. 2009 Apr 15; 57(6):583-91.GLIA

Abstract

Many studies have shown that adenosine triphosphate (ATP), as a neurotransmitter, is involved in plastic changes of synaptic transmission in central nervous system. In the present study, we tested whether extracellular ATP can induce long-term potentiation (LTP) of C-fiber-evoked field potentials in spinal dorsal horn. The results showed the following: (1) ATP at a concentration of 0.3 mM induced spinal LTP of C-fiber-evoked field potentials, lasting for at least 5 h; (2) spinal application of 2',3'-O-(2,4,6-trinitrophenyl)adenosine-5-triphosphate (TNP-ATP; an antagonist of P2X(1-4) receptors), but not pyridoxal-phosphate-6-azophenyl-2',4'-disulphonic acid (PPADS; an antagonist of P2X(1,2,3,5,7) receptors), 30 min before ATP blocked ATP-induced LTP, indicating that ATP may induce spinal LTP by activation of P2X(4) receptors; (3) at 60 min after LTP induction the level of phospho-p38 mitogen-activated protein kinase (p-p38 MAPK) was significantly elevated and at 180 min after LTP the number of P2X(4) receptors increased significantly; both p-p38 and P2X(4) receptors were exclusively co-located with the microglia marker, but not with neuronal or astrocyte marker; (4) spinal application of TNP-ATP but not PPADS prevented p38 activation; (5) spinal application of SB203580, a p38 MAPK inhibitor, prevented both spinal LTP and the upregulation of P2X(4) receptors. The results suggested that ATP may activate p38 MAPK by binding to intrinsic P2X(4) receptors in microglia, and subsequently enhance the expression of P2X(4) receptors, contributing to spinal LTP.

Authors+Show Affiliations

Department of Physiology and Pain Research Center, Zhongshan Medical School, Sun Yat-Sen University, Guangzhou 510080, People's Republic of China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18837052

Citation

Gong, Qing-Juan, et al. "ATP Induces Long-term Potentiation of C-fiber-evoked Field Potentials in Spinal Dorsal Horn: the Roles of P2X4 Receptors and P38 MAPK in Microglia." Glia, vol. 57, no. 6, 2009, pp. 583-91.
Gong QJ, Li YY, Xin WJ, et al. ATP induces long-term potentiation of C-fiber-evoked field potentials in spinal dorsal horn: the roles of P2X4 receptors and p38 MAPK in microglia. Glia. 2009;57(6):583-91.
Gong, Q. J., Li, Y. Y., Xin, W. J., Zang, Y., Ren, W. J., Wei, X. H., Li, Y. Y., Zhang, T., & Liu, X. G. (2009). ATP induces long-term potentiation of C-fiber-evoked field potentials in spinal dorsal horn: the roles of P2X4 receptors and p38 MAPK in microglia. Glia, 57(6), 583-91. https://doi.org/10.1002/glia.20786
Gong QJ, et al. ATP Induces Long-term Potentiation of C-fiber-evoked Field Potentials in Spinal Dorsal Horn: the Roles of P2X4 Receptors and P38 MAPK in Microglia. Glia. 2009 Apr 15;57(6):583-91. PubMed PMID: 18837052.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - ATP induces long-term potentiation of C-fiber-evoked field potentials in spinal dorsal horn: the roles of P2X4 receptors and p38 MAPK in microglia. AU - Gong,Qing-Juan, AU - Li,Yu-Ying, AU - Xin,Wen-Jun, AU - Zang,Ying, AU - Ren,Wen-Jie, AU - Wei,Xu-Hong, AU - Li,Yong-Yong, AU - Zhang,Tong, AU - Liu,Xian-Guo, PY - 2008/10/7/pubmed PY - 2009/5/14/medline PY - 2008/10/7/entrez SP - 583 EP - 91 JF - Glia JO - Glia VL - 57 IS - 6 N2 - Many studies have shown that adenosine triphosphate (ATP), as a neurotransmitter, is involved in plastic changes of synaptic transmission in central nervous system. In the present study, we tested whether extracellular ATP can induce long-term potentiation (LTP) of C-fiber-evoked field potentials in spinal dorsal horn. The results showed the following: (1) ATP at a concentration of 0.3 mM induced spinal LTP of C-fiber-evoked field potentials, lasting for at least 5 h; (2) spinal application of 2',3'-O-(2,4,6-trinitrophenyl)adenosine-5-triphosphate (TNP-ATP; an antagonist of P2X(1-4) receptors), but not pyridoxal-phosphate-6-azophenyl-2',4'-disulphonic acid (PPADS; an antagonist of P2X(1,2,3,5,7) receptors), 30 min before ATP blocked ATP-induced LTP, indicating that ATP may induce spinal LTP by activation of P2X(4) receptors; (3) at 60 min after LTP induction the level of phospho-p38 mitogen-activated protein kinase (p-p38 MAPK) was significantly elevated and at 180 min after LTP the number of P2X(4) receptors increased significantly; both p-p38 and P2X(4) receptors were exclusively co-located with the microglia marker, but not with neuronal or astrocyte marker; (4) spinal application of TNP-ATP but not PPADS prevented p38 activation; (5) spinal application of SB203580, a p38 MAPK inhibitor, prevented both spinal LTP and the upregulation of P2X(4) receptors. The results suggested that ATP may activate p38 MAPK by binding to intrinsic P2X(4) receptors in microglia, and subsequently enhance the expression of P2X(4) receptors, contributing to spinal LTP. SN - 1098-1136 UR - https://www.unboundmedicine.com/medline/citation/18837052/ATP_induces_long_term_potentiation_of_C_fiber_evoked_field_potentials_in_spinal_dorsal_horn:_the_roles_of_P2X4_receptors_and_p38_MAPK_in_microglia_ L2 - https://doi.org/10.1002/glia.20786 DB - PRIME DP - Unbound Medicine ER -