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Detection of circulating antibody of Parastrongylus cantonensis in sera with eosinophilic meningitis by dot-blot ELISA.
J Med Assoc Thai. 2008 Jul; 91(7):1082-6.JM

Abstract

BACKGROUND

Eosinophilic meningitis caused by Parastrongylus cantonensis, the rat lung worm is a major public health problem in Thailand. Humans acquire this parasite by eating raw food containing infective Parastrongyliasis is dificult to make because identification of parasite materials by biopsy or chance finding is rarely possible.

OBJECTIVE

Develop alternative approaches of Parastrongylus cantonensis infection employing crude antigen by dot-blot ELISA.

MATERIAL AND METHOD

The investigation was carried out between October 2003 and July 2004 in Khon Kaen, which is an endemic area. One hundred thirty two serum samples from several villagers of the present study area were divided into five groups. Group 1 consisted of 30 patients with Cryptococcal meningitis, group 2 were 22 cases of Bacterial meningitis, group 3 were 32 cases of eosinophilic meningitis, group 4 were other parasitic infections (4 from Cysticercosis, 2 from Fascioliasis, 12 from Malaria), and group 5 were 30 negative healthy control.

RESULTS

The result demonstrated that 26 cases of eosinophilic meningitis, were positive with Dot-blot ELISA (81.3%). None of the other groups of sample reacted with this antigen.

CONCLUSION

The data obtained showed that Dot-blot ELISA has a potential for diagnosis of eosinophilic meningitis caused by Parastrongylus cantonensis.

Authors+Show Affiliations

Division of Immunology, Department of Clinical Pathology, Khon Kaen Hospital, Khon Kaen, Thailand. kanchanatomanakan@yahoo.comNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18839849

Citation

Tomanakan, Kanchana, et al. "Detection of Circulating Antibody of Parastrongylus Cantonensis in Sera With Eosinophilic Meningitis By Dot-blot ELISA." Journal of the Medical Association of Thailand = Chotmaihet Thangphaet, vol. 91, no. 7, 2008, pp. 1082-6.
Tomanakan K, Srisurach N, Sae-tung S, et al. Detection of circulating antibody of Parastrongylus cantonensis in sera with eosinophilic meningitis by dot-blot ELISA. J Med Assoc Thai. 2008;91(7):1082-6.
Tomanakan, K., Srisurach, N., Sae-tung, S., & Pengpinich, C. (2008). Detection of circulating antibody of Parastrongylus cantonensis in sera with eosinophilic meningitis by dot-blot ELISA. Journal of the Medical Association of Thailand = Chotmaihet Thangphaet, 91(7), 1082-6.
Tomanakan K, et al. Detection of Circulating Antibody of Parastrongylus Cantonensis in Sera With Eosinophilic Meningitis By Dot-blot ELISA. J Med Assoc Thai. 2008;91(7):1082-6. PubMed PMID: 18839849.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Detection of circulating antibody of Parastrongylus cantonensis in sera with eosinophilic meningitis by dot-blot ELISA. AU - Tomanakan,Kanchana, AU - Srisurach,Nattiya, AU - Sae-tung,Surin, AU - Pengpinich,Churat, PY - 2008/10/9/pubmed PY - 2008/11/19/medline PY - 2008/10/9/entrez SP - 1082 EP - 6 JF - Journal of the Medical Association of Thailand = Chotmaihet thangphaet JO - J Med Assoc Thai VL - 91 IS - 7 N2 - BACKGROUND: Eosinophilic meningitis caused by Parastrongylus cantonensis, the rat lung worm is a major public health problem in Thailand. Humans acquire this parasite by eating raw food containing infective Parastrongyliasis is dificult to make because identification of parasite materials by biopsy or chance finding is rarely possible. OBJECTIVE: Develop alternative approaches of Parastrongylus cantonensis infection employing crude antigen by dot-blot ELISA. MATERIAL AND METHOD: The investigation was carried out between October 2003 and July 2004 in Khon Kaen, which is an endemic area. One hundred thirty two serum samples from several villagers of the present study area were divided into five groups. Group 1 consisted of 30 patients with Cryptococcal meningitis, group 2 were 22 cases of Bacterial meningitis, group 3 were 32 cases of eosinophilic meningitis, group 4 were other parasitic infections (4 from Cysticercosis, 2 from Fascioliasis, 12 from Malaria), and group 5 were 30 negative healthy control. RESULTS: The result demonstrated that 26 cases of eosinophilic meningitis, were positive with Dot-blot ELISA (81.3%). None of the other groups of sample reacted with this antigen. CONCLUSION: The data obtained showed that Dot-blot ELISA has a potential for diagnosis of eosinophilic meningitis caused by Parastrongylus cantonensis. SN - 0125-2208 UR - https://www.unboundmedicine.com/medline/citation/18839849/Detection_of_circulating_antibody_of_Parastrongylus_cantonensis_in_sera_with_eosinophilic_meningitis_by_dot_blot_ELISA_ L2 - https://medlineplus.gov/meningitis.html DB - PRIME DP - Unbound Medicine ER -