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Changes in the activities of the enzymes of urea synthesis caused by dexamethasone and dibutyryladenosine 3':5'-cyclic monophosphate in foetal rat liver maintained in organ culture.
Biochem J. 1976 Nov 15; 160(2):159-62.BJ

Abstract

Liver explants from 19-day foetal rats were maintained in organ culture, in a defined medium, for up to 48h. Both 6-N,2'-O-dibutyryl cyclic AMP, in the presence of theophylline, and dexamethasone caused an increase in the activities of carbamoyl phosphate synthase, argininosuccinate synthetase, argininosuccinate lyase and arginase. These increases could be abolished by simultaneously incubating the explants with cycloheximide. No change in the activity of ornithine transcarbamoylase was found with either hormone. Previous work has shown that injection of corticosteroids into 19.5-day foetal rats in utero did not cause an increase in the arginine synthetase system. Present results suggest that this lack of effect is not due to any incompetence of the foetal rat liver at this stage to respond to this agent. The observations on ornithine transcarbamoylase activity suggest that this enzyme is induced in the liver of the perinatal rat by neither corticosteroids nor hormones acting via cyclic AMP, and it may be that all the enzymes of the urea cycle are induced physiologically by an agent or agents as yet unidentified.

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Authors

Edkins E
No affiliation info available
Rïhä NC
No affiliation info available

MeSH

AnimalsArginaseArgininosuccinate LyaseArgininosuccinate SynthaseBucladesineCarbamoyl-Phosphate Synthase (Ammonia)CycloheximideDexamethasoneEnzyme InductionLiverOrgan Culture TechniquesOrnithine CarbamoyltransferaseRatsTheophyllineUrea

Pub Type(s)

Journal Article

Language

eng

PubMed ID

188416

Citation

Edkins, E, and N C. Rïhä. "Changes in the Activities of the Enzymes of Urea Synthesis Caused By Dexamethasone and Dibutyryladenosine 3':5'-cyclic Monophosphate in Foetal Rat Liver Maintained in Organ Culture." The Biochemical Journal, vol. 160, no. 2, 1976, pp. 159-62.
Edkins E, Rïhä NC. Changes in the activities of the enzymes of urea synthesis caused by dexamethasone and dibutyryladenosine 3':5'-cyclic monophosphate in foetal rat liver maintained in organ culture. Biochem J. 1976;160(2):159-62.
Edkins, E., & Rïhä, N. C. (1976). Changes in the activities of the enzymes of urea synthesis caused by dexamethasone and dibutyryladenosine 3':5'-cyclic monophosphate in foetal rat liver maintained in organ culture. The Biochemical Journal, 160(2), 159-62.
Edkins E, Rïhä NC. Changes in the Activities of the Enzymes of Urea Synthesis Caused By Dexamethasone and Dibutyryladenosine 3':5'-cyclic Monophosphate in Foetal Rat Liver Maintained in Organ Culture. Biochem J. 1976 Nov 15;160(2):159-62. PubMed PMID: 188416.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Changes in the activities of the enzymes of urea synthesis caused by dexamethasone and dibutyryladenosine 3':5'-cyclic monophosphate in foetal rat liver maintained in organ culture. AU - Edkins,E, AU - Rïhä,N C, PY - 1976/11/15/pubmed PY - 1976/11/15/medline PY - 1976/11/15/entrez SP - 159 EP - 62 JF - The Biochemical journal JO - Biochem J VL - 160 IS - 2 N2 - Liver explants from 19-day foetal rats were maintained in organ culture, in a defined medium, for up to 48h. Both 6-N,2'-O-dibutyryl cyclic AMP, in the presence of theophylline, and dexamethasone caused an increase in the activities of carbamoyl phosphate synthase, argininosuccinate synthetase, argininosuccinate lyase and arginase. These increases could be abolished by simultaneously incubating the explants with cycloheximide. No change in the activity of ornithine transcarbamoylase was found with either hormone. Previous work has shown that injection of corticosteroids into 19.5-day foetal rats in utero did not cause an increase in the arginine synthetase system. Present results suggest that this lack of effect is not due to any incompetence of the foetal rat liver at this stage to respond to this agent. The observations on ornithine transcarbamoylase activity suggest that this enzyme is induced in the liver of the perinatal rat by neither corticosteroids nor hormones acting via cyclic AMP, and it may be that all the enzymes of the urea cycle are induced physiologically by an agent or agents as yet unidentified. SN - 0264-6021 UR - https://www.unboundmedicine.com/medline/citation/188416/Changes_in_the_activities_of_the_enzymes_of_urea_synthesis_caused_by_dexamethasone_and_dibutyryladenosine_3':5'_cyclic_monophosphate_in_foetal_rat_liver_maintained_in_organ_culture_ L2 - https://portlandpress.com/biochemj/article-lookup/doi/10.1042/bj1600159 DB - PRIME DP - Unbound Medicine ER -