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[Limited determination of trace toxical aconitine alkaloid in guifudihuang pills].
Zhongguo Zhong Yao Za Zhi. 2008 Jul; 33(14):1684-7.ZZ

Abstract

OBJECTIVE

To establish a capillary electrophoresis with field-amplified sample stacking method for the separation and determination of aconitine alkaloid in Guifudihuang pills.

METHOD

An uncoated fused-silica capillary column (50 microm x 43 cm, effective length 35 cm) was used. The running buffer was 50 mmol x L(-1) phosphate electrolyte solution (pH 4.6)-methanol (8:2). The runing voltage was 10 kV and the capillary inlet was dipped in methanol for 5 s prior to electrokinetic injection (10 kV, 40 s). The detection wavelength was 235 nm.

RESULT

This method allowed 500 fold enrichment of aconitine alkaloid. A good linear relation was obtained in the range of 31.3-2 x 10(3) microg x L(-1) (r = 0.9996), with the detection limit of 9.4 microg x L(-1). The average recovery was 98.0% with the RSD of 2.6%.

CONCLUSION

The method is simple, rapid and specific with high stacking efficiency; it provides a new reliable means for production and quality control of Guifudihuang pills.

Authors+Show Affiliations

School of Pharmacy, Hebei Medical University, Shijiazhuang 050017, China.No affiliation info available

Pub Type(s)

English Abstract
Journal Article

Language

chi

PubMed ID

18841765

Citation

Li, Jun-mei, and Ye Jiang. "[Limited Determination of Trace Toxical Aconitine Alkaloid in Guifudihuang Pills]." Zhongguo Zhong Yao Za Zhi = Zhongguo Zhongyao Zazhi = China Journal of Chinese Materia Medica, vol. 33, no. 14, 2008, pp. 1684-7.
Li JM, Jiang Y. [Limited determination of trace toxical aconitine alkaloid in guifudihuang pills]. Zhongguo Zhong Yao Za Zhi. 2008;33(14):1684-7.
Li, J. M., & Jiang, Y. (2008). [Limited determination of trace toxical aconitine alkaloid in guifudihuang pills]. Zhongguo Zhong Yao Za Zhi = Zhongguo Zhongyao Zazhi = China Journal of Chinese Materia Medica, 33(14), 1684-7.
Li JM, Jiang Y. [Limited Determination of Trace Toxical Aconitine Alkaloid in Guifudihuang Pills]. Zhongguo Zhong Yao Za Zhi. 2008;33(14):1684-7. PubMed PMID: 18841765.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Limited determination of trace toxical aconitine alkaloid in guifudihuang pills]. AU - Li,Jun-mei, AU - Jiang,Ye, PY - 2008/10/10/pubmed PY - 2009/5/15/medline PY - 2008/10/10/entrez SP - 1684 EP - 7 JF - Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica JO - Zhongguo Zhong Yao Za Zhi VL - 33 IS - 14 N2 - OBJECTIVE: To establish a capillary electrophoresis with field-amplified sample stacking method for the separation and determination of aconitine alkaloid in Guifudihuang pills. METHOD: An uncoated fused-silica capillary column (50 microm x 43 cm, effective length 35 cm) was used. The running buffer was 50 mmol x L(-1) phosphate electrolyte solution (pH 4.6)-methanol (8:2). The runing voltage was 10 kV and the capillary inlet was dipped in methanol for 5 s prior to electrokinetic injection (10 kV, 40 s). The detection wavelength was 235 nm. RESULT: This method allowed 500 fold enrichment of aconitine alkaloid. A good linear relation was obtained in the range of 31.3-2 x 10(3) microg x L(-1) (r = 0.9996), with the detection limit of 9.4 microg x L(-1). The average recovery was 98.0% with the RSD of 2.6%. CONCLUSION: The method is simple, rapid and specific with high stacking efficiency; it provides a new reliable means for production and quality control of Guifudihuang pills. SN - 1001-5302 UR - https://www.unboundmedicine.com/medline/citation/18841765/[Limited_determination_of_trace_toxical_aconitine_alkaloid_in_guifudihuang_pills]_ DB - PRIME DP - Unbound Medicine ER -
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