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Effects of extraction methods on phenolic contents and antioxidant activity in aerial parts of Potentilla atrosanguinea Lodd. and quantification of its phenolic constituents by RP-HPLC.
J Agric Food Chem. 2008 Nov 12; 56(21):10129-34.JA

Abstract

The effects of different solvent systems (methanol, ethanol, acetone, and their 50% aqueous concentrations) and extraction procedures (microwave, ultrasound, Soxhlet and maceration) on the antioxidant activity of aerial parts of Potentilla atrosanguinea were investigated by three different bioassays: 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS), 2,2'-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assays and ferric reducing antioxidant potential (FRAP). The 50% aqueous ethanol extracts exhibited strong antioxidant activity measured in terms of Trolox equivalent antioxidant capacity (TEAC) [(54.34 to 122.96, 29.82 to 101.22 and 13.64 to 41.43) mg of Trolox/g] with ABTS (*+), DPPH (*) and FRAP assays, respectively. In general, TEAC of Soxhlet extracts was found to be 1.8 and 3 times higher than ultrasound and maceration but slightly (1.2 times) higher than microwave. A positive correlation (r(2) = 0.931 to 0.982) was observed between total polyphenol (TPC) and total flavonoid (TFC) contents which ranged between 26.7 to 30.7 mg/g gallic acid equivalent and 16.8 to 20.8 mg/g quercetin equivalent respectively, with antioxidant activity. In addition, some of its bioactive phenolic constituents which contribute largely toward antioxidant potential such as chlorogenic acid, catechin, caffeic acid, p-coumaric acid and quercetin were also quantified in different extracts by RP-HPLC.

Authors+Show Affiliations

Natural Plant Products Division, Institute of Himalayan Bioresource Technology (CSIR), Post Box 6, Palampur-176 061, Himachal Pradesh, India.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18841977

Citation

Kalia, Kalpana, et al. "Effects of Extraction Methods On Phenolic Contents and Antioxidant Activity in Aerial Parts of Potentilla Atrosanguinea Lodd. and Quantification of Its Phenolic Constituents By RP-HPLC." Journal of Agricultural and Food Chemistry, vol. 56, no. 21, 2008, pp. 10129-34.
Kalia K, Sharma K, Singh HP, et al. Effects of extraction methods on phenolic contents and antioxidant activity in aerial parts of Potentilla atrosanguinea Lodd. and quantification of its phenolic constituents by RP-HPLC. J Agric Food Chem. 2008;56(21):10129-34.
Kalia, K., Sharma, K., Singh, H. P., & Singh, B. (2008). Effects of extraction methods on phenolic contents and antioxidant activity in aerial parts of Potentilla atrosanguinea Lodd. and quantification of its phenolic constituents by RP-HPLC. Journal of Agricultural and Food Chemistry, 56(21), 10129-34. https://doi.org/10.1021/jf802188b
Kalia K, et al. Effects of Extraction Methods On Phenolic Contents and Antioxidant Activity in Aerial Parts of Potentilla Atrosanguinea Lodd. and Quantification of Its Phenolic Constituents By RP-HPLC. J Agric Food Chem. 2008 Nov 12;56(21):10129-34. PubMed PMID: 18841977.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effects of extraction methods on phenolic contents and antioxidant activity in aerial parts of Potentilla atrosanguinea Lodd. and quantification of its phenolic constituents by RP-HPLC. AU - Kalia,Kalpana, AU - Sharma,Kapil, AU - Singh,Harsh Pratap, AU - Singh,Bikram, Y1 - 2008/10/09/ PY - 2008/10/10/pubmed PY - 2009/1/1/medline PY - 2008/10/10/entrez SP - 10129 EP - 34 JF - Journal of agricultural and food chemistry JO - J. Agric. Food Chem. VL - 56 IS - 21 N2 - The effects of different solvent systems (methanol, ethanol, acetone, and their 50% aqueous concentrations) and extraction procedures (microwave, ultrasound, Soxhlet and maceration) on the antioxidant activity of aerial parts of Potentilla atrosanguinea were investigated by three different bioassays: 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS), 2,2'-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assays and ferric reducing antioxidant potential (FRAP). The 50% aqueous ethanol extracts exhibited strong antioxidant activity measured in terms of Trolox equivalent antioxidant capacity (TEAC) [(54.34 to 122.96, 29.82 to 101.22 and 13.64 to 41.43) mg of Trolox/g] with ABTS (*+), DPPH (*) and FRAP assays, respectively. In general, TEAC of Soxhlet extracts was found to be 1.8 and 3 times higher than ultrasound and maceration but slightly (1.2 times) higher than microwave. A positive correlation (r(2) = 0.931 to 0.982) was observed between total polyphenol (TPC) and total flavonoid (TFC) contents which ranged between 26.7 to 30.7 mg/g gallic acid equivalent and 16.8 to 20.8 mg/g quercetin equivalent respectively, with antioxidant activity. In addition, some of its bioactive phenolic constituents which contribute largely toward antioxidant potential such as chlorogenic acid, catechin, caffeic acid, p-coumaric acid and quercetin were also quantified in different extracts by RP-HPLC. SN - 1520-5118 UR - https://www.unboundmedicine.com/medline/citation/18841977/Effects_of_extraction_methods_on_phenolic_contents_and_antioxidant_activity_in_aerial_parts_of_Potentilla_atrosanguinea_Lodd__and_quantification_of_its_phenolic_constituents_by_RP_HPLC_ L2 - https://dx.doi.org/10.1021/jf802188b DB - PRIME DP - Unbound Medicine ER -