Tags

Type your tag names separated by a space and hit enter

Identification of minimal predictors of avian pathogenic Escherichia coli virulence for use as a rapid diagnostic tool.
J Clin Microbiol. 2008 Dec; 46(12):3987-96.JC

Abstract

To identify traits that predict avian pathogenic Escherichia coli (APEC) virulence, 124 avian E. coli isolates of known pathogenicity and serogroup were subjected to virulence genotyping and phylogenetic typing. The results were analyzed by multiple-correspondence analysis. From this analysis, five genes carried by plasmids were identified as being the most significantly associated with highly pathogenic APEC strains: iutA, hlyF, iss, iroN, and ompT. A multiplex PCR panel targeting these five genes was used to screen a collection of 994 avian E. coli isolates. APEC isolates were clearly distinguished from the avian fecal E. coli isolates by their possession of these genes, suggesting that this pentaplex panel has diagnostic applications and underscoring the close association between avian E. coli virulence and the possession of ColV plasmids. Also, the sharp demarcation between APEC isolates and avian fecal E. coli isolates in their plasmid-associated virulence gene content suggests that APEC isolates are well equipped for a pathogenic lifestyle, which is contrary to the widely held belief that most APEC isolates are opportunistic pathogens. Regardless, APEC isolates remain an important problem for poultry producers and a potential concern for public health professionals, as growing evidence suggests a possible role for APEC in human disease. Thus, the pentaplex panel described here may be useful in detecting APEC-like strains occurring in poultry production, along the food chain, and in human disease. This panel may be helpful toward clarifying potential roles of APEC in human disease, ascertaining the source of APEC in animal outbreaks, and identifying effective targets of avian colibacillosis control.

Authors+Show Affiliations

Department of Veterinary and Biomedical Sciences, University of Minnesota, St. Paul, Minnesota 55108, USA. .No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18842938

Citation

Johnson, Timothy J., et al. "Identification of Minimal Predictors of Avian Pathogenic Escherichia Coli Virulence for Use as a Rapid Diagnostic Tool." Journal of Clinical Microbiology, vol. 46, no. 12, 2008, pp. 3987-96.
Johnson TJ, Wannemuehler Y, Doetkott C, et al. Identification of minimal predictors of avian pathogenic Escherichia coli virulence for use as a rapid diagnostic tool. J Clin Microbiol. 2008;46(12):3987-96.
Johnson, T. J., Wannemuehler, Y., Doetkott, C., Johnson, S. J., Rosenberger, S. C., & Nolan, L. K. (2008). Identification of minimal predictors of avian pathogenic Escherichia coli virulence for use as a rapid diagnostic tool. Journal of Clinical Microbiology, 46(12), 3987-96. https://doi.org/10.1128/JCM.00816-08
Johnson TJ, et al. Identification of Minimal Predictors of Avian Pathogenic Escherichia Coli Virulence for Use as a Rapid Diagnostic Tool. J Clin Microbiol. 2008;46(12):3987-96. PubMed PMID: 18842938.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Identification of minimal predictors of avian pathogenic Escherichia coli virulence for use as a rapid diagnostic tool. AU - Johnson,Timothy J, AU - Wannemuehler,Yvonne, AU - Doetkott,Curt, AU - Johnson,Sara J, AU - Rosenberger,Sandra C, AU - Nolan,Lisa K, Y1 - 2008/10/08/ PY - 2008/10/10/pubmed PY - 2008/12/25/medline PY - 2008/10/10/entrez SP - 3987 EP - 96 JF - Journal of clinical microbiology JO - J Clin Microbiol VL - 46 IS - 12 N2 - To identify traits that predict avian pathogenic Escherichia coli (APEC) virulence, 124 avian E. coli isolates of known pathogenicity and serogroup were subjected to virulence genotyping and phylogenetic typing. The results were analyzed by multiple-correspondence analysis. From this analysis, five genes carried by plasmids were identified as being the most significantly associated with highly pathogenic APEC strains: iutA, hlyF, iss, iroN, and ompT. A multiplex PCR panel targeting these five genes was used to screen a collection of 994 avian E. coli isolates. APEC isolates were clearly distinguished from the avian fecal E. coli isolates by their possession of these genes, suggesting that this pentaplex panel has diagnostic applications and underscoring the close association between avian E. coli virulence and the possession of ColV plasmids. Also, the sharp demarcation between APEC isolates and avian fecal E. coli isolates in their plasmid-associated virulence gene content suggests that APEC isolates are well equipped for a pathogenic lifestyle, which is contrary to the widely held belief that most APEC isolates are opportunistic pathogens. Regardless, APEC isolates remain an important problem for poultry producers and a potential concern for public health professionals, as growing evidence suggests a possible role for APEC in human disease. Thus, the pentaplex panel described here may be useful in detecting APEC-like strains occurring in poultry production, along the food chain, and in human disease. This panel may be helpful toward clarifying potential roles of APEC in human disease, ascertaining the source of APEC in animal outbreaks, and identifying effective targets of avian colibacillosis control. SN - 1098-660X UR - https://www.unboundmedicine.com/medline/citation/18842938/Identification_of_minimal_predictors_of_avian_pathogenic_Escherichia_coli_virulence_for_use_as_a_rapid_diagnostic_tool_ L2 - http://jcm.asm.org/cgi/pmidlookup?view=long&pmid=18842938 DB - PRIME DP - Unbound Medicine ER -