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Global identification of significantly expressed genes in developing endosperm of rice by expression sequence tags and cDNA array approaches.
J Integr Plant Biol. 2008 Sep; 50(9):1078-88.JI

Abstract

Rice endosperm plays a very important role in seedling germination and determines the qualities of rice grain. Although studies on specific gene categories in endosperm have been carried out, global view of gene expression at a transcription level in rice endosperm is still limited. To gain a better understanding of the global and tissue-specific gene expression profiles in rice endosperm, a cDNA library from rice endosperm of immature seeds was sequenced. A cDNA array was constructed based on the tentative unique transcripts derived from expression sequence tag (EST) assembling results and then hybridized with cDNAs from five different tissues or organs including endosperm, embryo, leaf, stem and root of rice. Significant redundancy was found for genes encoding prolamin, glutelin, allergen, and starch synthesis proteins, accounting for approximately 34% of the total ESTs obtained. The cDNA array revealed 87 significantly expressed genes in endosperm compared with the other four organs or tissues. These genes included 13 prolamin family proteins, 17 glutelin family proteins, 12 binding proteins, nine catalytic proteins and four ribosomal proteins, indicating a complicated biological processing in rice endosperm. In addition, Northern verification of 1,4-alpha-glucan branching enzyme detected two isoforms in rice endosperm, the larger one of which only existed in endosperm.

Authors+Show Affiliations

Bioinformatics and Gene Network Research Group, Institute of Biotechnology, Zhejiang University, Hangzhou 310029, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18844776

Citation

Tu, Qichao, et al. "Global Identification of Significantly Expressed Genes in Developing Endosperm of Rice By Expression Sequence Tags and cDNA Array Approaches." Journal of Integrative Plant Biology, vol. 50, no. 9, 2008, pp. 1078-88.
Tu Q, Dong H, Yao H, et al. Global identification of significantly expressed genes in developing endosperm of rice by expression sequence tags and cDNA array approaches. J Integr Plant Biol. 2008;50(9):1078-88.
Tu, Q., Dong, H., Yao, H., Fang, Y., Dai, C., Luo, H., Yao, J., Zhao, D., & Li, D. (2008). Global identification of significantly expressed genes in developing endosperm of rice by expression sequence tags and cDNA array approaches. Journal of Integrative Plant Biology, 50(9), 1078-88. https://doi.org/10.1111/j.1744-7909.2008.00714.x
Tu Q, et al. Global Identification of Significantly Expressed Genes in Developing Endosperm of Rice By Expression Sequence Tags and cDNA Array Approaches. J Integr Plant Biol. 2008;50(9):1078-88. PubMed PMID: 18844776.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Global identification of significantly expressed genes in developing endosperm of rice by expression sequence tags and cDNA array approaches. AU - Tu,Qichao, AU - Dong,Haitao, AU - Yao,Haigen, AU - Fang,Yongqi, AU - Dai,Cheng'en, AU - Luo,Hongmei, AU - Yao,Jian, AU - Zhao,Dong, AU - Li,Debao, PY - 2008/10/11/pubmed PY - 2009/1/24/medline PY - 2008/10/11/entrez SP - 1078 EP - 88 JF - Journal of integrative plant biology JO - J Integr Plant Biol VL - 50 IS - 9 N2 - Rice endosperm plays a very important role in seedling germination and determines the qualities of rice grain. Although studies on specific gene categories in endosperm have been carried out, global view of gene expression at a transcription level in rice endosperm is still limited. To gain a better understanding of the global and tissue-specific gene expression profiles in rice endosperm, a cDNA library from rice endosperm of immature seeds was sequenced. A cDNA array was constructed based on the tentative unique transcripts derived from expression sequence tag (EST) assembling results and then hybridized with cDNAs from five different tissues or organs including endosperm, embryo, leaf, stem and root of rice. Significant redundancy was found for genes encoding prolamin, glutelin, allergen, and starch synthesis proteins, accounting for approximately 34% of the total ESTs obtained. The cDNA array revealed 87 significantly expressed genes in endosperm compared with the other four organs or tissues. These genes included 13 prolamin family proteins, 17 glutelin family proteins, 12 binding proteins, nine catalytic proteins and four ribosomal proteins, indicating a complicated biological processing in rice endosperm. In addition, Northern verification of 1,4-alpha-glucan branching enzyme detected two isoforms in rice endosperm, the larger one of which only existed in endosperm. SN - 1744-7909 UR - https://www.unboundmedicine.com/medline/citation/18844776/Global_identification_of_significantly_expressed_genes_in_developing_endosperm_of_rice_by_expression_sequence_tags_and_cDNA_array_approaches_ L2 - https://doi.org/10.1111/j.1744-7909.2008.00714.x DB - PRIME DP - Unbound Medicine ER -