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Functional Epstein-Barr virus reservoir in plasma cells derived from infected peripheral blood memory B cells.
Blood 2009; 113(3):604-11Blood

Abstract

The Epstein-Barr virus (EBV) causes infectious mononucleosis, establishes latency in resting memory B lymphocytes, and is involved in oncogenesis through poorly understood mechanisms. The EBV lytic cycle is initiated during plasma cell differentiation by mRNAs transcripts encoded by BZLF1, which induce the synthesis of EBV proteins such as the immediate-early antigen ZEBRA and the late membrane antigen gp350. Therefore, we assessed the capacity of circulating EBV-infected B lymphocytes from healthy EBV-seropositive subjects to enter and complete the EBV lytic cycle. Purified B lymphocytes were polyclonally stimulated and BZLF1- or gp350-secreting cells (BZLF1-SCs or gp350-SCs) were enumerated by ELISpot assays. The number of BZLF1-SCs ranged from 50 to 480/107 lymphocytes (median, 80; 25th-75th percentiles, 70-150) and gp350-SCs from 10 to 40/107 lymphocytes (median, 17; 25th-75th percentiles, 10-20). gp350-SCs represented only 7.7% to 28.6% of BZLF1-SCs (median, 15%; 25th-75th percentiles, 10.5%-20%). This EBV functional reservoir was preferentially restricted to plasma cells derived from CD27(+) IgD(-) memory B lymphocytes. In 9 of 13 subjects, EBV DNA quantification in B-cell culture supernatants gave evidence of completion of EBV lytic cycle. These results demonstrate that EBV proteins can be secreted by EBV-infected B lymphocytes from healthy carriers, a majority generating an abortive EBV lytic cycle and a minority completing the cycle.

Authors+Show Affiliations

Department of Virology, University Medical Center, Montpellier, France.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18845794

Citation

Al Tabaa, Yassine, et al. "Functional Epstein-Barr Virus Reservoir in Plasma Cells Derived From Infected Peripheral Blood Memory B Cells." Blood, vol. 113, no. 3, 2009, pp. 604-11.
Al Tabaa Y, Tuaillon E, Bollore K, et al. Functional Epstein-Barr virus reservoir in plasma cells derived from infected peripheral blood memory B cells. Blood. 2009;113(3):604-11.
Al Tabaa, Y., Tuaillon, E., Bollore, K., Foulongne, V., Petitjean, G., Seigneurin, J. M., ... Vendrell, J. P. (2009). Functional Epstein-Barr virus reservoir in plasma cells derived from infected peripheral blood memory B cells. Blood, 113(3), pp. 604-11. doi:10.1182/blood-2008-02-136903.
Al Tabaa Y, et al. Functional Epstein-Barr Virus Reservoir in Plasma Cells Derived From Infected Peripheral Blood Memory B Cells. Blood. 2009 Jan 15;113(3):604-11. PubMed PMID: 18845794.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Functional Epstein-Barr virus reservoir in plasma cells derived from infected peripheral blood memory B cells. AU - Al Tabaa,Yassine, AU - Tuaillon,Edouard, AU - Bollore,Karine, AU - Foulongne,Vincent, AU - Petitjean,Gael, AU - Seigneurin,Jean-Marie, AU - Duperray,Christophe, AU - Desgranges,Claude, AU - Vendrell,Jean-Pierre, Y1 - 2008/10/09/ PY - 2008/10/11/pubmed PY - 2009/2/20/medline PY - 2008/10/11/entrez SP - 604 EP - 11 JF - Blood JO - Blood VL - 113 IS - 3 N2 - The Epstein-Barr virus (EBV) causes infectious mononucleosis, establishes latency in resting memory B lymphocytes, and is involved in oncogenesis through poorly understood mechanisms. The EBV lytic cycle is initiated during plasma cell differentiation by mRNAs transcripts encoded by BZLF1, which induce the synthesis of EBV proteins such as the immediate-early antigen ZEBRA and the late membrane antigen gp350. Therefore, we assessed the capacity of circulating EBV-infected B lymphocytes from healthy EBV-seropositive subjects to enter and complete the EBV lytic cycle. Purified B lymphocytes were polyclonally stimulated and BZLF1- or gp350-secreting cells (BZLF1-SCs or gp350-SCs) were enumerated by ELISpot assays. The number of BZLF1-SCs ranged from 50 to 480/107 lymphocytes (median, 80; 25th-75th percentiles, 70-150) and gp350-SCs from 10 to 40/107 lymphocytes (median, 17; 25th-75th percentiles, 10-20). gp350-SCs represented only 7.7% to 28.6% of BZLF1-SCs (median, 15%; 25th-75th percentiles, 10.5%-20%). This EBV functional reservoir was preferentially restricted to plasma cells derived from CD27(+) IgD(-) memory B lymphocytes. In 9 of 13 subjects, EBV DNA quantification in B-cell culture supernatants gave evidence of completion of EBV lytic cycle. These results demonstrate that EBV proteins can be secreted by EBV-infected B lymphocytes from healthy carriers, a majority generating an abortive EBV lytic cycle and a minority completing the cycle. SN - 1528-0020 UR - https://www.unboundmedicine.com/medline/citation/18845794/Functional_Epstein_Barr_virus_reservoir_in_plasma_cells_derived_from_infected_peripheral_blood_memory_B_cells_ L2 - http://www.bloodjournal.org/cgi/pmidlookup?view=long&pmid=18845794 DB - PRIME DP - Unbound Medicine ER -