TY - JOUR T1 - Antibodies and CD4(+) T-cells mediate cross-protection against H5N1 influenza virus infection in mice after vaccination with a low pathogenic H5N2 strain. AU - Droebner,Karoline, AU - Haasbach,Emanuel, AU - Fuchs,Cordula, AU - Weinzierl,Andreas O, AU - Stevanovic,Stefan, AU - Büttner,Mathias, AU - Planz,Oliver, Y1 - 2008/10/09/ PY - 2008/04/21/received PY - 2008/09/10/revised PY - 2008/09/17/accepted PY - 2008/10/14/pubmed PY - 2009/2/20/medline PY - 2008/10/14/entrez SP - 6965 EP - 74 JF - Vaccine JO - Vaccine VL - 26 IS - 52 N2 - A H5N2 low pathogenic avian influenza virus (LPAIV) was isolated from a natural reservoir in Bavaria during a routine screen and was used as a vaccine strain to scrutinize the immune response involved in cross-protection after challenge infection with a H5N1 highly pathogenic avian influenza virus (HPAIV). The challenge virus was also isolated from a natural reservoir in Bavaria. Wild type, antibody deficient (muMT), CD4(-/-) and CD8(-/-) mice were infected with the apathogenic H5N2 vaccine strain and challenge infection with a 100-fold MLD(50) of the H5N1 strain was performed 80 days later. While 100% of the wild type and 100% of the CD8(-/-) mice stayed healthy, only 50% of the CD4(-/-) and none of the antibody deficient mice were protected. These results support the view that the humoral immune response and to certain extends the CD4(+) T helper cells are a prerequisite for cross-protective immunity against H5 influenza virus. SN - 0264-410X UR - https://www.unboundmedicine.com/medline/citation/18848593/Antibodies_and_CD4_+__T_cells_mediate_cross_protection_against_H5N1_influenza_virus_infection_in_mice_after_vaccination_with_a_low_pathogenic_H5N2_strain_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0264-410X(08)01311-X DB - PRIME DP - Unbound Medicine ER -