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IgE-induced degranulation of mucosal mast cells is negatively regulated via nicotinic acetylcholine receptors.
Biochem Biophys Res Commun. 2008 Dec 05; 377(1):321-5.BB

Abstract

The autonomic nervous system is known to mediate mast cell activation. We investigated expression of nicotinic acetylcholine receptors (nAChRs) in mucosal-type mast cells and their contribution to the regulation of mast cell activation. Expression of mRNA of nAChR alpha4, alpha7, and beta2 subunits were detected in specially differentiated mucosal-type murine bone marrow-derived mast cells (mBMMCs). Pretreatment with non-specific nAChRs agonists, acetylcholine, nicotine and epibatidine and a specific alpha7 subunit agonist GTS-21 significantly inhibited antigen-induced degranulation of mBMMCs in a dose-dependent manner and GTS-21-induced inhibition was significantly blocked by alpha7 subunit antagonist, alpha-bungarotoxin. Furthermore, confocal microscopy also demonstrated surface binding of alpha-bungarotoxin on mBMMCs. Our findings indicate that mucosal mast cell activation may be negatively regulated mainly through nAChR alpha7 subunit, suggesting that nAChRs are involved in neuronal-mucosal mast cell interactions.

Authors+Show Affiliations

Department of Bioscience, Division of Gastrointestinal Pathophysiology, Institute of Natural Medicine, University of Toyama, Sugitani, Toyama, Japan. natsuko@inm.u-toyama.ac.jpNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18848921

Citation

Kageyama-Yahara, Natsuko, et al. "IgE-induced Degranulation of Mucosal Mast Cells Is Negatively Regulated Via Nicotinic Acetylcholine Receptors." Biochemical and Biophysical Research Communications, vol. 377, no. 1, 2008, pp. 321-5.
Kageyama-Yahara N, Suehiro Y, Yamamoto T, et al. IgE-induced degranulation of mucosal mast cells is negatively regulated via nicotinic acetylcholine receptors. Biochem Biophys Res Commun. 2008;377(1):321-5.
Kageyama-Yahara, N., Suehiro, Y., Yamamoto, T., & Kadowaki, M. (2008). IgE-induced degranulation of mucosal mast cells is negatively regulated via nicotinic acetylcholine receptors. Biochemical and Biophysical Research Communications, 377(1), 321-5. https://doi.org/10.1016/j.bbrc.2008.10.004
Kageyama-Yahara N, et al. IgE-induced Degranulation of Mucosal Mast Cells Is Negatively Regulated Via Nicotinic Acetylcholine Receptors. Biochem Biophys Res Commun. 2008 Dec 5;377(1):321-5. PubMed PMID: 18848921.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - IgE-induced degranulation of mucosal mast cells is negatively regulated via nicotinic acetylcholine receptors. AU - Kageyama-Yahara,Natsuko, AU - Suehiro,Yoko, AU - Yamamoto,Takeshi, AU - Kadowaki,Makoto, Y1 - 2008/10/10/ PY - 2008/10/02/received PY - 2008/10/03/accepted PY - 2008/10/14/pubmed PY - 2008/12/17/medline PY - 2008/10/14/entrez SP - 321 EP - 5 JF - Biochemical and biophysical research communications JO - Biochem Biophys Res Commun VL - 377 IS - 1 N2 - The autonomic nervous system is known to mediate mast cell activation. We investigated expression of nicotinic acetylcholine receptors (nAChRs) in mucosal-type mast cells and their contribution to the regulation of mast cell activation. Expression of mRNA of nAChR alpha4, alpha7, and beta2 subunits were detected in specially differentiated mucosal-type murine bone marrow-derived mast cells (mBMMCs). Pretreatment with non-specific nAChRs agonists, acetylcholine, nicotine and epibatidine and a specific alpha7 subunit agonist GTS-21 significantly inhibited antigen-induced degranulation of mBMMCs in a dose-dependent manner and GTS-21-induced inhibition was significantly blocked by alpha7 subunit antagonist, alpha-bungarotoxin. Furthermore, confocal microscopy also demonstrated surface binding of alpha-bungarotoxin on mBMMCs. Our findings indicate that mucosal mast cell activation may be negatively regulated mainly through nAChR alpha7 subunit, suggesting that nAChRs are involved in neuronal-mucosal mast cell interactions. SN - 1090-2104 UR - https://www.unboundmedicine.com/medline/citation/18848921/IgE_induced_degranulation_of_mucosal_mast_cells_is_negatively_regulated_via_nicotinic_acetylcholine_receptors_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0006-291X(08)01970-0 DB - PRIME DP - Unbound Medicine ER -