Tags

Type your tag names separated by a space and hit enter

High-performance liquid chromatographic and pharmacokinetic analyses of an intravenous submicron emulsion of perillyl alcohol in rats.
J Pharm Biomed Anal. 2008 Dec 01; 48(4):1201-5.JP

Abstract

Perillyl alcohol (POH) is currently in phase II clinical trials both as a chemopreventative and chemotherapeutic agent. The present report describes a simple, rapid and sensitive HPLC-UV method to quantify POH in rat plasma. After protein precipitation with acetonitrile, POH was separated using an Agilent Zorbax XDB C(18) column (150 mm x 4.6 mm, 5 microm) with a mobile phase consisting of acetonitrile-water (40:60, v/v) at a flow rate of 1.0 ml min(-1), and detected at 210 nm. The method has been used successfully to determine trace levels of POH in plasma down to 0.015 microg ml(-1). The pharmacokinetics of POH after intravenous administrations in three formulations, i.e. POH solution (POH-SOL), negatively charged submicron emulsions (POH-SE) and positively charged submicron emulsions (POH-CSSE) were investigated. AUC(0-infinity), MRT, t(1/2alpha) and t(1/2beta) of POH-SE and POH-CSSE were significantly higher, while their total body clearance was lower than those of POH-SOL. In addition, AUC(0-infinity), MRT and t(1/2beta) of POH-CSSE were significantly higher than those of POH-SE. The results indicate that the submicron emulsion formulation significantly increases POH blood concentrations and retention within the systemic circulation.

Authors+Show Affiliations

Academy of Medical and Pharmaceutical Sciences, Zhengzhou University, 40 Daxue Road, Zhengzhou, Henan Province 450052, PR China. hhylu@yahoo.com.cnNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

18849133

Citation

Hua, Hai-Ying, et al. "High-performance Liquid Chromatographic and Pharmacokinetic Analyses of an Intravenous Submicron Emulsion of Perillyl Alcohol in Rats." Journal of Pharmaceutical and Biomedical Analysis, vol. 48, no. 4, 2008, pp. 1201-5.
Hua HY, Zhao YX, Liu L, et al. High-performance liquid chromatographic and pharmacokinetic analyses of an intravenous submicron emulsion of perillyl alcohol in rats. J Pharm Biomed Anal. 2008;48(4):1201-5.
Hua, H. Y., Zhao, Y. X., Liu, L., Ye, Q. X., & Ge, S. W. (2008). High-performance liquid chromatographic and pharmacokinetic analyses of an intravenous submicron emulsion of perillyl alcohol in rats. Journal of Pharmaceutical and Biomedical Analysis, 48(4), 1201-5. https://doi.org/10.1016/j.jpba.2008.08.015
Hua HY, et al. High-performance Liquid Chromatographic and Pharmacokinetic Analyses of an Intravenous Submicron Emulsion of Perillyl Alcohol in Rats. J Pharm Biomed Anal. 2008 Dec 1;48(4):1201-5. PubMed PMID: 18849133.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - High-performance liquid chromatographic and pharmacokinetic analyses of an intravenous submicron emulsion of perillyl alcohol in rats. AU - Hua,Hai-Ying, AU - Zhao,Yong-Xing, AU - Liu,Lin, AU - Ye,Qi-Xia, AU - Ge,Shi-Wen, Y1 - 2008/08/22/ PY - 2008/06/05/received PY - 2008/07/28/revised PY - 2008/08/05/accepted PY - 2008/10/14/pubmed PY - 2009/4/1/medline PY - 2008/10/14/entrez SP - 1201 EP - 5 JF - Journal of pharmaceutical and biomedical analysis JO - J Pharm Biomed Anal VL - 48 IS - 4 N2 - Perillyl alcohol (POH) is currently in phase II clinical trials both as a chemopreventative and chemotherapeutic agent. The present report describes a simple, rapid and sensitive HPLC-UV method to quantify POH in rat plasma. After protein precipitation with acetonitrile, POH was separated using an Agilent Zorbax XDB C(18) column (150 mm x 4.6 mm, 5 microm) with a mobile phase consisting of acetonitrile-water (40:60, v/v) at a flow rate of 1.0 ml min(-1), and detected at 210 nm. The method has been used successfully to determine trace levels of POH in plasma down to 0.015 microg ml(-1). The pharmacokinetics of POH after intravenous administrations in three formulations, i.e. POH solution (POH-SOL), negatively charged submicron emulsions (POH-SE) and positively charged submicron emulsions (POH-CSSE) were investigated. AUC(0-infinity), MRT, t(1/2alpha) and t(1/2beta) of POH-SE and POH-CSSE were significantly higher, while their total body clearance was lower than those of POH-SOL. In addition, AUC(0-infinity), MRT and t(1/2beta) of POH-CSSE were significantly higher than those of POH-SE. The results indicate that the submicron emulsion formulation significantly increases POH blood concentrations and retention within the systemic circulation. SN - 0731-7085 UR - https://www.unboundmedicine.com/medline/citation/18849133/High_performance_liquid_chromatographic_and_pharmacokinetic_analyses_of_an_intravenous_submicron_emulsion_of_perillyl_alcohol_in_rats_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0731-7085(08)00406-8 DB - PRIME DP - Unbound Medicine ER -