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Intercellular space diameters of the oesophageal epithelium in NERD patients: head to head comparison between light and electron microscopy analysis.
Dig Liver Dis 2009; 41(1):9-14DL

Abstract

BACKGROUND

Dilation of intercellular space diameters of oesophageal epithelium detected at transmission electron microscopy morphometry is a marker of tissue injury in non-erosive reflux disease patients. Semi-quantitative evaluation of intercellular space diameters using light microscopy seems to provide promising results.

AIM/METHODS

To comparatively evaluate intercellular space diameters in the same patients, by means of morphometry and semi-quantitative analysis, both on light microscopy and transmission electron microscopy microphotographs, biopsies were taken in 29 non-erosive reflux disease patients at distal and proximal oesophagus. Twelve asymptomatic controls underwent the same protocol.

RESULTS

Morphometric analysis on transmission electron microscopy microphotographs showed mean intercellular space diameter values of patients, at distal and proximal oesophagus, 3- and 2-fold, respectively, higher than those in controls (p<0.001). On light microscopy microphotographs, mean intercellular space diameter values of patients at distal oesophagus were higher than those in controls, an overlap between patients and controls being observed. The semi-quantitative score was positive in 79% of patients and in 25% of controls at distal esophagus.

CONCLUSIONS

Intercellular space diameter morphometric analysis at light microscopy is widely available, allows intercellular space diameter to be quantitatively measured with good sensitivity and specificity and could represent a useful tool in non-erosive reflux disease diagnosis. Despite satisfactory sensitivity, the semi-quantitative score at light microscopy is hampered by much lower specificity than transmission electron microscopy- and light microscopy-morphometry.

Authors+Show Affiliations

Department of Digestive Diseases, Campus Bio Medico University, Rome, Italy.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Evaluation Study
Journal Article

Language

eng

PubMed ID

18849206

Citation

Ribolsi, M, et al. "Intercellular Space Diameters of the Oesophageal Epithelium in NERD Patients: Head to Head Comparison Between Light and Electron Microscopy Analysis." Digestive and Liver Disease : Official Journal of the Italian Society of Gastroenterology and the Italian Association for the Study of the Liver, vol. 41, no. 1, 2009, pp. 9-14.
Ribolsi M, Perrone G, Caviglia R, et al. Intercellular space diameters of the oesophageal epithelium in NERD patients: head to head comparison between light and electron microscopy analysis. Dig Liver Dis. 2009;41(1):9-14.
Ribolsi, M., Perrone, G., Caviglia, R., Gentile, M., Emerenziani, S., Luca Guarino, M. P., ... Cicala, M. (2009). Intercellular space diameters of the oesophageal epithelium in NERD patients: head to head comparison between light and electron microscopy analysis. Digestive and Liver Disease : Official Journal of the Italian Society of Gastroenterology and the Italian Association for the Study of the Liver, 41(1), pp. 9-14. doi:10.1016/j.dld.2008.07.318.
Ribolsi M, et al. Intercellular Space Diameters of the Oesophageal Epithelium in NERD Patients: Head to Head Comparison Between Light and Electron Microscopy Analysis. Dig Liver Dis. 2009;41(1):9-14. PubMed PMID: 18849206.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Intercellular space diameters of the oesophageal epithelium in NERD patients: head to head comparison between light and electron microscopy analysis. AU - Ribolsi,M, AU - Perrone,G, AU - Caviglia,R, AU - Gentile,M, AU - Emerenziani,S, AU - Luca Guarino,M P, AU - Petitti,T, AU - Cicala,M, Y1 - 2008/10/11/ PY - 2008/05/09/received PY - 2008/07/24/revised PY - 2008/07/25/accepted PY - 2008/10/14/pubmed PY - 2009/3/13/medline PY - 2008/10/14/entrez SP - 9 EP - 14 JF - Digestive and liver disease : official journal of the Italian Society of Gastroenterology and the Italian Association for the Study of the Liver JO - Dig Liver Dis VL - 41 IS - 1 N2 - BACKGROUND: Dilation of intercellular space diameters of oesophageal epithelium detected at transmission electron microscopy morphometry is a marker of tissue injury in non-erosive reflux disease patients. Semi-quantitative evaluation of intercellular space diameters using light microscopy seems to provide promising results. AIM/METHODS: To comparatively evaluate intercellular space diameters in the same patients, by means of morphometry and semi-quantitative analysis, both on light microscopy and transmission electron microscopy microphotographs, biopsies were taken in 29 non-erosive reflux disease patients at distal and proximal oesophagus. Twelve asymptomatic controls underwent the same protocol. RESULTS: Morphometric analysis on transmission electron microscopy microphotographs showed mean intercellular space diameter values of patients, at distal and proximal oesophagus, 3- and 2-fold, respectively, higher than those in controls (p<0.001). On light microscopy microphotographs, mean intercellular space diameter values of patients at distal oesophagus were higher than those in controls, an overlap between patients and controls being observed. The semi-quantitative score was positive in 79% of patients and in 25% of controls at distal esophagus. CONCLUSIONS: Intercellular space diameter morphometric analysis at light microscopy is widely available, allows intercellular space diameter to be quantitatively measured with good sensitivity and specificity and could represent a useful tool in non-erosive reflux disease diagnosis. Despite satisfactory sensitivity, the semi-quantitative score at light microscopy is hampered by much lower specificity than transmission electron microscopy- and light microscopy-morphometry. SN - 1878-3562 UR - https://www.unboundmedicine.com/medline/citation/18849206/Intercellular_space_diameters_of_the_oesophageal_epithelium_in_NERD_patients:_head_to_head_comparison_between_light_and_electron_microscopy_analysis_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1590-8658(08)00602-6 DB - PRIME DP - Unbound Medicine ER -