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Detection of staphylococcal cassette chromosome mec-associated DNA segments in multiresistant methicillin-susceptible Staphylococcus aureus (MSSA) and identification of Staphylococcus epidermidis ccrAB4 in both methicillin-resistant S. aureus and MSSA.
Antimicrob Agents Chemother. 2008 Dec; 52(12):4407-19.AA

Abstract

Methicillin-susceptible Staphylococcus aureus (MSSA) can arise from methicillin-resistant S. aureus (MRSA) following partial or complete excision of staphylococcal cassette chromosome mec (SCCmec). This study investigated whether multiresistant MSSA isolates from Irish hospitals, where MRSA has been endemic for decades, harbor SCCmec DNA. Twenty-five multiresistant MSSA isolates recovered between 2002 and 2006 were tested for SCCmec DNA by PCR and were genotyped by multilocus sequence typing and spa typing. All isolates lacked mecA. Three isolates (12%) harbored SCCmec DNA; two of these (genotype ST8/t190) harbored a 26-kb SCCmec IID (II.3.1.2) remnant that lacked part of mecI and all of mecR1, mecA, and IS431; the third isolate (ST8/t3209) harbored the SCCmec region from dcs to orfX. All three isolates were detected as MRSA using the BD GeneOhm and Cepheid's Xpert MRSA real-time PCR assays. Six isolates (ST8/t190, n = 4; ST5/t088, n = 2), including both isolates with the SCCmec IID remnant, harbored ccrAB4 with 100% identity to ccrAB4 from the Staphylococcus epidermidis composite island SCC-CI. This ccrAB4 gene was also identified in 23 MRSA isolates representative of ST8/t190-MRSA with variant SCCmec II subtypes IIA to IIE, which predominated previously in Irish hospitals. ccrAB4 was located 5,549 bp upstream of the left SCCmec junction in both the MRSA and MSSA isolates with SCCmec elements and remnants and 5,549 bp upstream of orfX in the four MSSA isolates with ccrAB4 only on an SCC-CI homologous region. This is the first description of a large SCCmec remnant with ccr and partial mec genes in MSSA and of the S. epidermidis SCC-CI and ccrAB4 genes in S. aureus.

Authors+Show Affiliations

Microbiology Research Unit, Division of Oral Biosciences, School of Dental Science and Dublin Dental Hospital, University of Dublin, Trinity College Dublin, Dublin 2, Ireland.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18852274

Citation

Shore, Anna C., et al. "Detection of Staphylococcal Cassette Chromosome Mec-associated DNA Segments in Multiresistant Methicillin-susceptible Staphylococcus Aureus (MSSA) and Identification of Staphylococcus Epidermidis ccrAB4 in Both Methicillin-resistant S. Aureus and MSSA." Antimicrobial Agents and Chemotherapy, vol. 52, no. 12, 2008, pp. 4407-19.
Shore AC, Rossney AS, O'Connell B, et al. Detection of staphylococcal cassette chromosome mec-associated DNA segments in multiresistant methicillin-susceptible Staphylococcus aureus (MSSA) and identification of Staphylococcus epidermidis ccrAB4 in both methicillin-resistant S. aureus and MSSA. Antimicrob Agents Chemother. 2008;52(12):4407-19.
Shore, A. C., Rossney, A. S., O'Connell, B., Herra, C. M., Sullivan, D. J., Humphreys, H., & Coleman, D. C. (2008). Detection of staphylococcal cassette chromosome mec-associated DNA segments in multiresistant methicillin-susceptible Staphylococcus aureus (MSSA) and identification of Staphylococcus epidermidis ccrAB4 in both methicillin-resistant S. aureus and MSSA. Antimicrobial Agents and Chemotherapy, 52(12), 4407-19. https://doi.org/10.1128/AAC.00447-08
Shore AC, et al. Detection of Staphylococcal Cassette Chromosome Mec-associated DNA Segments in Multiresistant Methicillin-susceptible Staphylococcus Aureus (MSSA) and Identification of Staphylococcus Epidermidis ccrAB4 in Both Methicillin-resistant S. Aureus and MSSA. Antimicrob Agents Chemother. 2008;52(12):4407-19. PubMed PMID: 18852274.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Detection of staphylococcal cassette chromosome mec-associated DNA segments in multiresistant methicillin-susceptible Staphylococcus aureus (MSSA) and identification of Staphylococcus epidermidis ccrAB4 in both methicillin-resistant S. aureus and MSSA. AU - Shore,Anna C, AU - Rossney,Angela S, AU - O'Connell,Brian, AU - Herra,Celine M, AU - Sullivan,Derek J, AU - Humphreys,Hilary, AU - Coleman,David C, Y1 - 2008/10/13/ PY - 2008/10/15/pubmed PY - 2009/1/28/medline PY - 2008/10/15/entrez SP - 4407 EP - 19 JF - Antimicrobial agents and chemotherapy JO - Antimicrob Agents Chemother VL - 52 IS - 12 N2 - Methicillin-susceptible Staphylococcus aureus (MSSA) can arise from methicillin-resistant S. aureus (MRSA) following partial or complete excision of staphylococcal cassette chromosome mec (SCCmec). This study investigated whether multiresistant MSSA isolates from Irish hospitals, where MRSA has been endemic for decades, harbor SCCmec DNA. Twenty-five multiresistant MSSA isolates recovered between 2002 and 2006 were tested for SCCmec DNA by PCR and were genotyped by multilocus sequence typing and spa typing. All isolates lacked mecA. Three isolates (12%) harbored SCCmec DNA; two of these (genotype ST8/t190) harbored a 26-kb SCCmec IID (II.3.1.2) remnant that lacked part of mecI and all of mecR1, mecA, and IS431; the third isolate (ST8/t3209) harbored the SCCmec region from dcs to orfX. All three isolates were detected as MRSA using the BD GeneOhm and Cepheid's Xpert MRSA real-time PCR assays. Six isolates (ST8/t190, n = 4; ST5/t088, n = 2), including both isolates with the SCCmec IID remnant, harbored ccrAB4 with 100% identity to ccrAB4 from the Staphylococcus epidermidis composite island SCC-CI. This ccrAB4 gene was also identified in 23 MRSA isolates representative of ST8/t190-MRSA with variant SCCmec II subtypes IIA to IIE, which predominated previously in Irish hospitals. ccrAB4 was located 5,549 bp upstream of the left SCCmec junction in both the MRSA and MSSA isolates with SCCmec elements and remnants and 5,549 bp upstream of orfX in the four MSSA isolates with ccrAB4 only on an SCC-CI homologous region. This is the first description of a large SCCmec remnant with ccr and partial mec genes in MSSA and of the S. epidermidis SCC-CI and ccrAB4 genes in S. aureus. SN - 1098-6596 UR - https://www.unboundmedicine.com/medline/citation/18852274/Detection_of_staphylococcal_cassette_chromosome_mec_associated_DNA_segments_in_multiresistant_methicillin_susceptible_Staphylococcus_aureus__MSSA__and_identification_of_Staphylococcus_epidermidis_ccrAB4_in_both_methicillin_resistant_S__aureus_and_MSSA_ L2 - http://aac.asm.org/cgi/pmidlookup?view=long&pmid=18852274 DB - PRIME DP - Unbound Medicine ER -