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Detection of antibody responses and delayed dermal hypersensitivity with Blastomyces dermatitidis yeast and mycelial lysate antigens.
Mycopathologia. 1991 Jun; 114(3):137-44.M

Abstract

Yeast cell lysate and mycelial lysate antigens prepared from one strain (T-58) of Blastomyces dermatitidis were evaluated with respect to the detection of antibodies and delayed dermal hypersensitivity. Comparable ELISA sensitivity values were evidenced with the two antigens when assayed against serum specimens from dogs with blastomycosis, sera from non-infected dogs residing in endemic and non-endemic areas for blastomycosis and sera from rabbits that were hyperimmunized with B. dermatitidis antigens. Specificity determinations with anti-Histoplasma capsulatum rabbit sera indicated that both reagents exhibited only minimal cross-reactivity; the mycelial antigen was slightly more specific than the yeast phase reagent. Similar sensitivity and specificity results were experienced when the two antigens were used to detect delayed dermal hypersensitivity in guinea pigs previously sensitized with B. dermatitidis or H. capsulatum.

Authors+Show Affiliations

Seawell BW
Department of Biological Sciences, Idaho State University, Pocatello.
Wheeler JY
No affiliation info available
Yearsley K
No affiliation info available
Alexander KL
No affiliation info available
Legendre AM
No affiliation info available
Scalarone GM
No affiliation info available

MeSH

AnimalsAntibodies, FungalAntigens, FungalBlastomycesBlastomycosisCross ReactionsDog DiseasesDogsEnzyme-Linked Immunosorbent AssayGuinea PigsHistoplasmaHypersensitivity, DelayedImmune SeraRabbitsSkin

Pub Type(s)

Journal Article

Language

eng

PubMed ID

1886639

Citation

Seawell, B W., et al. "Detection of Antibody Responses and Delayed Dermal Hypersensitivity With Blastomyces Dermatitidis Yeast and Mycelial Lysate Antigens." Mycopathologia, vol. 114, no. 3, 1991, pp. 137-44.
Seawell BW, Wheeler JY, Yearsley K, et al. Detection of antibody responses and delayed dermal hypersensitivity with Blastomyces dermatitidis yeast and mycelial lysate antigens. Mycopathologia. 1991;114(3):137-44.
Seawell, B. W., Wheeler, J. Y., Yearsley, K., Alexander, K. L., Legendre, A. M., & Scalarone, G. M. (1991). Detection of antibody responses and delayed dermal hypersensitivity with Blastomyces dermatitidis yeast and mycelial lysate antigens. Mycopathologia, 114(3), 137-44.
Seawell BW, et al. Detection of Antibody Responses and Delayed Dermal Hypersensitivity With Blastomyces Dermatitidis Yeast and Mycelial Lysate Antigens. Mycopathologia. 1991;114(3):137-44. PubMed PMID: 1886639.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Detection of antibody responses and delayed dermal hypersensitivity with Blastomyces dermatitidis yeast and mycelial lysate antigens. AU - Seawell,B W, AU - Wheeler,J Y, AU - Yearsley,K, AU - Alexander,K L, AU - Legendre,A M, AU - Scalarone,G M, PY - 1991/6/1/pubmed PY - 1991/6/1/medline PY - 1991/6/1/entrez SP - 137 EP - 44 JF - Mycopathologia JO - Mycopathologia VL - 114 IS - 3 N2 - Yeast cell lysate and mycelial lysate antigens prepared from one strain (T-58) of Blastomyces dermatitidis were evaluated with respect to the detection of antibodies and delayed dermal hypersensitivity. Comparable ELISA sensitivity values were evidenced with the two antigens when assayed against serum specimens from dogs with blastomycosis, sera from non-infected dogs residing in endemic and non-endemic areas for blastomycosis and sera from rabbits that were hyperimmunized with B. dermatitidis antigens. Specificity determinations with anti-Histoplasma capsulatum rabbit sera indicated that both reagents exhibited only minimal cross-reactivity; the mycelial antigen was slightly more specific than the yeast phase reagent. Similar sensitivity and specificity results were experienced when the two antigens were used to detect delayed dermal hypersensitivity in guinea pigs previously sensitized with B. dermatitidis or H. capsulatum. SN - 0301-486X UR - https://www.unboundmedicine.com/medline/citation/1886639/Detection_of_antibody_responses_and_delayed_dermal_hypersensitivity_with_Blastomyces_dermatitidis_yeast_and_mycelial_lysate_antigens_ DB - PRIME DP - Unbound Medicine ER -