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Detection of M. leprae-specific antigens with dot-ELISA in urine and nasal samples from leprosy patients.
Int J Lepr Other Mycobact Dis. 1991 Sep; 59(3):398-404.IJ

Abstract

One-hundred-two urine and nasal samples collected from leprosy patients of different classifications of disease were studied for the presence of Mycobacterium leprae antigens, including phenolic glycolipid-I (PGL-I). Lipids were extracted from the urine samples, and nasal washings were concentrated and used as such in the dot-ELISA. Two types of primary antibodies, a polyclonal antibody obtained from lepromatous (LL) leprosy patients' pooled and absorbed sera and an anti-PGL-I monoclonal antibody, were used for the detection of M. leprae antigens from these samples. The polyclonal sera detected 23% to 36% of the paucibacillary (PB) and 100% of the multibacillary (MB) leprosy cases from the urine samples. Corresponding values for nasal detection were 10% to 18% for PB and 100% for MB cases. The monoclonal antibody against PGL-I could not detect tuberculoid (TT) leprosy cases. From the urine samples, however, 16% of the borderline tuberculoid (BT), 25% of the borderline (BB), 80% of the borderline lepromatous (BL), and 100% of the LL leprosy cases were detectable. It was interesting to note that PB, skin-smear negative cases were detectable from urine examination. The specificity and sensitivity of the test is discussed in relation to the crossreacting antigens.

Authors+Show Affiliations

Division of Microbiology, Central Drug Research Institute, Lucknow, India.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

1890363

Citation

Singh, N B., et al. "Detection of M. Leprae-specific Antigens With dot-ELISA in Urine and Nasal Samples From Leprosy Patients." International Journal of Leprosy and Other Mycobacterial Diseases : Official Organ of the International Leprosy Association, vol. 59, no. 3, 1991, pp. 398-404.
Singh NB, Choudhary A, Bhatnagar S. Detection of M. leprae-specific antigens with dot-ELISA in urine and nasal samples from leprosy patients. Int J Lepr Other Mycobact Dis. 1991;59(3):398-404.
Singh, N. B., Choudhary, A., & Bhatnagar, S. (1991). Detection of M. leprae-specific antigens with dot-ELISA in urine and nasal samples from leprosy patients. International Journal of Leprosy and Other Mycobacterial Diseases : Official Organ of the International Leprosy Association, 59(3), 398-404.
Singh NB, Choudhary A, Bhatnagar S. Detection of M. Leprae-specific Antigens With dot-ELISA in Urine and Nasal Samples From Leprosy Patients. Int J Lepr Other Mycobact Dis. 1991;59(3):398-404. PubMed PMID: 1890363.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Detection of M. leprae-specific antigens with dot-ELISA in urine and nasal samples from leprosy patients. AU - Singh,N B, AU - Choudhary,A, AU - Bhatnagar,S, PY - 1991/9/1/pubmed PY - 1991/9/1/medline PY - 1991/9/1/entrez SP - 398 EP - 404 JF - International journal of leprosy and other mycobacterial diseases : official organ of the International Leprosy Association JO - Int J Lepr Other Mycobact Dis VL - 59 IS - 3 N2 - One-hundred-two urine and nasal samples collected from leprosy patients of different classifications of disease were studied for the presence of Mycobacterium leprae antigens, including phenolic glycolipid-I (PGL-I). Lipids were extracted from the urine samples, and nasal washings were concentrated and used as such in the dot-ELISA. Two types of primary antibodies, a polyclonal antibody obtained from lepromatous (LL) leprosy patients' pooled and absorbed sera and an anti-PGL-I monoclonal antibody, were used for the detection of M. leprae antigens from these samples. The polyclonal sera detected 23% to 36% of the paucibacillary (PB) and 100% of the multibacillary (MB) leprosy cases from the urine samples. Corresponding values for nasal detection were 10% to 18% for PB and 100% for MB cases. The monoclonal antibody against PGL-I could not detect tuberculoid (TT) leprosy cases. From the urine samples, however, 16% of the borderline tuberculoid (BT), 25% of the borderline (BB), 80% of the borderline lepromatous (BL), and 100% of the LL leprosy cases were detectable. It was interesting to note that PB, skin-smear negative cases were detectable from urine examination. The specificity and sensitivity of the test is discussed in relation to the crossreacting antigens. SN - 0148-916X UR - https://www.unboundmedicine.com/medline/citation/1890363/Detection_of_M__leprae_specific_antigens_with_dot_ELISA_in_urine_and_nasal_samples_from_leprosy_patients_ L2 - https://medlineplus.gov/mycobacterialinfections.html DB - PRIME DP - Unbound Medicine ER -