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In vivo genotoxicity testing of the amnesic shellfish poison (domoic acid) in piscine erythrocytes using the micronucleus test and the comet assay.
Aquat Toxicol. 2008 Nov 11; 90(2):154-9.AT

Abstract

Domoic acid (DA) is a neurotoxic amino acid naturally produced in the marine environment by some diatom species belonging to the genus Pseudo-nitzschia. Although the neurotoxic properties of DA have been demonstrated, very little is known about in vivo genotoxicity of DA on aquatic organisms. In the present paper, an in vivo study on the genotoxic effects of domoic acid was carried out on a fish, Oreochromis niloticus, using the micronucleus test and the comet assay. The fish were exposed to three doses of domoic acid (1, 5 and 10 microg/g body weight) by intracoelomic injections. Ethyl methane sulphonate at a single dose of 5mg/l was used as positive control. Analysis of micronuclei, nuclear abnormalities and DNA damage were carried out on peripheral erythrocytes sampled 24, 48 and 72 h post-treatment. Our results revealed significant increases in the frequencies of micronuclei, nuclear abnormalities as well as DNA strand breaks and thus demonstrated the genotoxic potential of DA on fish.

Authors+Show Affiliations

Mersin University, Faculty of Sciences and Letters, Department of Biology, 33343 Mersin, Turkey. tcavas@mersin.edu.trNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18922590

Citation

Cavaş, Tolga, and Serpil Könen. "In Vivo Genotoxicity Testing of the Amnesic Shellfish Poison (domoic Acid) in Piscine Erythrocytes Using the Micronucleus Test and the Comet Assay." Aquatic Toxicology (Amsterdam, Netherlands), vol. 90, no. 2, 2008, pp. 154-9.
Cavaş T, Könen S. In vivo genotoxicity testing of the amnesic shellfish poison (domoic acid) in piscine erythrocytes using the micronucleus test and the comet assay. Aquat Toxicol. 2008;90(2):154-9.
Cavaş, T., & Könen, S. (2008). In vivo genotoxicity testing of the amnesic shellfish poison (domoic acid) in piscine erythrocytes using the micronucleus test and the comet assay. Aquatic Toxicology (Amsterdam, Netherlands), 90(2), 154-9. https://doi.org/10.1016/j.aquatox.2008.08.011
Cavaş T, Könen S. In Vivo Genotoxicity Testing of the Amnesic Shellfish Poison (domoic Acid) in Piscine Erythrocytes Using the Micronucleus Test and the Comet Assay. Aquat Toxicol. 2008 Nov 11;90(2):154-9. PubMed PMID: 18922590.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - In vivo genotoxicity testing of the amnesic shellfish poison (domoic acid) in piscine erythrocytes using the micronucleus test and the comet assay. AU - Cavaş,Tolga, AU - Könen,Serpil, Y1 - 2008/09/05/ PY - 2008/04/09/received PY - 2008/08/15/revised PY - 2008/08/30/accepted PY - 2008/10/17/pubmed PY - 2008/12/17/medline PY - 2008/10/17/entrez SP - 154 EP - 9 JF - Aquatic toxicology (Amsterdam, Netherlands) JO - Aquat Toxicol VL - 90 IS - 2 N2 - Domoic acid (DA) is a neurotoxic amino acid naturally produced in the marine environment by some diatom species belonging to the genus Pseudo-nitzschia. Although the neurotoxic properties of DA have been demonstrated, very little is known about in vivo genotoxicity of DA on aquatic organisms. In the present paper, an in vivo study on the genotoxic effects of domoic acid was carried out on a fish, Oreochromis niloticus, using the micronucleus test and the comet assay. The fish were exposed to three doses of domoic acid (1, 5 and 10 microg/g body weight) by intracoelomic injections. Ethyl methane sulphonate at a single dose of 5mg/l was used as positive control. Analysis of micronuclei, nuclear abnormalities and DNA damage were carried out on peripheral erythrocytes sampled 24, 48 and 72 h post-treatment. Our results revealed significant increases in the frequencies of micronuclei, nuclear abnormalities as well as DNA strand breaks and thus demonstrated the genotoxic potential of DA on fish. SN - 1879-1514 UR - https://www.unboundmedicine.com/medline/citation/18922590/In_vivo_genotoxicity_testing_of_the_amnesic_shellfish_poison__domoic_acid__in_piscine_erythrocytes_using_the_micronucleus_test_and_the_comet_assay_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0166-445X(08)00272-5 DB - PRIME DP - Unbound Medicine ER -