Tags

Type your tag names separated by a space and hit enter

Comparative proteome analysis of robust Saccharomyces cerevisiae insights into industrial continuous and batch fermentation.
Appl Microbiol Biotechnol. 2008 Nov; 81(2):327-38.AM

Abstract

A robust Saccharomyces cerevisiae strain has been widely applied in continuous and batch/fed-batch industrial fermentation. However, little is known about the molecular basis of fermentative behavior of this strain in the two realistic fermentation processes. In this paper, we presented comparative proteomic profiling of the industrial yeast in the industrial fermentation processes. The expression levels of most identified protein were closely interrelated with the different stages of fermentation processes. Our results indicate that, among the 47 identified protein spots, 17 of them belonging to 12 enzymes were involved in pentose phosphate, glycolysis, and gluconeogenesis pathways and glycerol biosynthetic process, indicating that a number of pathways will need to be inactivated to improve ethanol production. The differential expressions of eight oxidative response and heat-shock proteins were also identified, suggesting that it is necessary to keep the correct cellular redox or osmotic state in the two industrial fermentation processes. Moreover, there are significant differences in changes of protein levels between the two industrial fermentation processes, especially these proteins associated with the glycolysis and gluconeogenesis pathways. These findings provide a molecular understanding of physiological adaptation of industrial strain for optimizing the performance of industrial bioethanol fermentation.

Links

Aggregator Full Text

Authors+Show Affiliations

Cheng JS
Key Laboratory of Systems Bioengineering, Tianjin University, Tianjin, People's Republic of China.
Qiao B
No affiliation info available
Yuan YJ
No affiliation info available

MeSH

EnzymesEthanolFermentationMetabolic Networks and PathwaysProteomeSaccharomyces cerevisiaeSaccharomyces cerevisiae Proteins

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18923828

Citation

Cheng, Jing-Sheng, et al. "Comparative Proteome Analysis of Robust Saccharomyces Cerevisiae Insights Into Industrial Continuous and Batch Fermentation." Applied Microbiology and Biotechnology, vol. 81, no. 2, 2008, pp. 327-38.
Cheng JS, Qiao B, Yuan YJ. Comparative proteome analysis of robust Saccharomyces cerevisiae insights into industrial continuous and batch fermentation. Appl Microbiol Biotechnol. 2008;81(2):327-38.
Cheng, J. S., Qiao, B., & Yuan, Y. J. (2008). Comparative proteome analysis of robust Saccharomyces cerevisiae insights into industrial continuous and batch fermentation. Applied Microbiology and Biotechnology, 81(2), 327-38. https://doi.org/10.1007/s00253-008-1733-6
Cheng JS, Qiao B, Yuan YJ. Comparative Proteome Analysis of Robust Saccharomyces Cerevisiae Insights Into Industrial Continuous and Batch Fermentation. Appl Microbiol Biotechnol. 2008;81(2):327-38. PubMed PMID: 18923828.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Comparative proteome analysis of robust Saccharomyces cerevisiae insights into industrial continuous and batch fermentation. AU - Cheng,Jing-Sheng, AU - Qiao,Bin, AU - Yuan,Ying-Jin, Y1 - 2008/10/16/ PY - 2008/08/15/received PY - 2008/09/25/accepted PY - 2008/09/22/revised PY - 2008/10/17/pubmed PY - 2008/12/17/medline PY - 2008/10/17/entrez SP - 327 EP - 38 JF - Applied microbiology and biotechnology JO - Appl Microbiol Biotechnol VL - 81 IS - 2 N2 - A robust Saccharomyces cerevisiae strain has been widely applied in continuous and batch/fed-batch industrial fermentation. However, little is known about the molecular basis of fermentative behavior of this strain in the two realistic fermentation processes. In this paper, we presented comparative proteomic profiling of the industrial yeast in the industrial fermentation processes. The expression levels of most identified protein were closely interrelated with the different stages of fermentation processes. Our results indicate that, among the 47 identified protein spots, 17 of them belonging to 12 enzymes were involved in pentose phosphate, glycolysis, and gluconeogenesis pathways and glycerol biosynthetic process, indicating that a number of pathways will need to be inactivated to improve ethanol production. The differential expressions of eight oxidative response and heat-shock proteins were also identified, suggesting that it is necessary to keep the correct cellular redox or osmotic state in the two industrial fermentation processes. Moreover, there are significant differences in changes of protein levels between the two industrial fermentation processes, especially these proteins associated with the glycolysis and gluconeogenesis pathways. These findings provide a molecular understanding of physiological adaptation of industrial strain for optimizing the performance of industrial bioethanol fermentation. SN - 1432-0614 UR - https://www.unboundmedicine.com/medline/citation/18923828/Comparative_proteome_analysis_of_robust_Saccharomyces_cerevisiae_insights_into_industrial_continuous_and_batch_fermentation_ L2 - https://dx.doi.org/10.1007/s00253-008-1733-6 DB - PRIME DP - Unbound Medicine ER -