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The YvfTU two-component system is involved in plcR expression in Bacillus cereus.
BMC Microbiol. 2008 Oct 16; 8:183.BM

Abstract

BACKGROUND

Most extracellular virulence factors produced by Bacillus cereus are regulated by the pleiotropic transcriptional activator PlcR. Among strains belonging to the B. cereus group, the plcR gene is always located in the vicinity of genes encoding the YvfTU two-component system. The putative role of YvfTU in the expression of the PlcR regulon was therefore investigated.

RESULTS

Expression of the plcR gene was monitored using a transcriptional fusion with a lacZ reporter gene in a yvfTU mutant and in its B. cereus ATCC 14579 parental strain. Two hours after the onset of the stationary phase, a stage at which the PlcR regulon is highly expressed, the plcR expression in the yvfTU mutant was only 50% of that of its parental strain. In addition to the reduced plcR expression in the yvfTU mutant, a few members of the PlcR regulon showed a differential expression, as revealed by transcriptomic and proteomic analyses. The virulence of the yvfTU mutant in a Galleria mellonella insect model was slightly lower than that of the parental strain.

CONCLUSION

The YvfTU two-component system is not required for the expression of most of the virulence factors belonging to the PlcR regulon. However, YvfTU is involved in expression of plcR, a major regulator of virulence in B. cereus.

Authors+Show Affiliations

UMR408, Sécurité et Qualité des Produits d'Origine Végétale, INRA, Université d'Avignon, F-84000 Avignon, France. brillard@avignon.inra.frNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18925929

Citation

Brillard, Julien, et al. "The YvfTU Two-component System Is Involved in plcR Expression in Bacillus Cereus." BMC Microbiology, vol. 8, 2008, p. 183.
Brillard J, Susanna K, Michaud C, et al. The YvfTU two-component system is involved in plcR expression in Bacillus cereus. BMC Microbiol. 2008;8:183.
Brillard, J., Susanna, K., Michaud, C., Dargaignaratz, C., Gohar, M., Nielsen-Leroux, C., Ramarao, N., Kolstø, A. B., Nguyen-the, C., Lereclus, D., & Broussolle, V. (2008). The YvfTU two-component system is involved in plcR expression in Bacillus cereus. BMC Microbiology, 8, 183. https://doi.org/10.1186/1471-2180-8-183
Brillard J, et al. The YvfTU Two-component System Is Involved in plcR Expression in Bacillus Cereus. BMC Microbiol. 2008 Oct 16;8:183. PubMed PMID: 18925929.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The YvfTU two-component system is involved in plcR expression in Bacillus cereus. AU - Brillard,Julien, AU - Susanna,Kim, AU - Michaud,Caroline, AU - Dargaignaratz,Claire, AU - Gohar,Michel, AU - Nielsen-Leroux,Christina, AU - Ramarao,Nalini, AU - Kolstø,Anne-Brit, AU - Nguyen-the,Christophe, AU - Lereclus,Didier, AU - Broussolle,Véronique, Y1 - 2008/10/16/ PY - 2008/05/29/received PY - 2008/10/16/accepted PY - 2008/10/18/pubmed PY - 2008/12/24/medline PY - 2008/10/18/entrez SP - 183 EP - 183 JF - BMC microbiology JO - BMC Microbiol. VL - 8 N2 - BACKGROUND: Most extracellular virulence factors produced by Bacillus cereus are regulated by the pleiotropic transcriptional activator PlcR. Among strains belonging to the B. cereus group, the plcR gene is always located in the vicinity of genes encoding the YvfTU two-component system. The putative role of YvfTU in the expression of the PlcR regulon was therefore investigated. RESULTS: Expression of the plcR gene was monitored using a transcriptional fusion with a lacZ reporter gene in a yvfTU mutant and in its B. cereus ATCC 14579 parental strain. Two hours after the onset of the stationary phase, a stage at which the PlcR regulon is highly expressed, the plcR expression in the yvfTU mutant was only 50% of that of its parental strain. In addition to the reduced plcR expression in the yvfTU mutant, a few members of the PlcR regulon showed a differential expression, as revealed by transcriptomic and proteomic analyses. The virulence of the yvfTU mutant in a Galleria mellonella insect model was slightly lower than that of the parental strain. CONCLUSION: The YvfTU two-component system is not required for the expression of most of the virulence factors belonging to the PlcR regulon. However, YvfTU is involved in expression of plcR, a major regulator of virulence in B. cereus. SN - 1471-2180 UR - https://www.unboundmedicine.com/medline/citation/18925929/The_YvfTU_two_component_system_is_involved_in_plcR_expression_in_Bacillus_cereus_ L2 - https://bmcmicrobiol.biomedcentral.com/articles/10.1186/1471-2180-8-183 DB - PRIME DP - Unbound Medicine ER -