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A histamine release assay to identify sensitization to Culicoides allergens in horses with skin hypersensitivity.
Vet Immunol Immunopathol. 2008 Dec 15; 126(3-4):302-8.VI

Abstract

Skin hypersensitivity is an allergic disease induced in horses by allergens of Culicoides midges. The condition is typically diagnosed by clinical signs and in some horses in combination with allergy testing such as intradermal skin testing or serological allergen-specific IgE determination. Here, we describe an alternative method for allergy testing: a histamine release assay (HRA) that combines the functional aspects of skin testing with the convenience of submitting a blood sample. The assay is based on the principle that crosslinking of allergen-specific IgE bound via high-affinity IgE receptors to the surfaces of mast cells and basophils induces the release of inflammatory mediators. One of these mediators is histamine. The histamine was then detected by a colorimetric enzyme-linked immunosorbent assay. The histamine assay was used to test 33 horses with skin hypersensitivity and 20 clinically healthy control animals for histamine release from their peripheral blood basophils after stimulation with Culicoides allergen extract or monoclonal anti-IgE antibody. An increased histamine release was observed in the horses with skin hypersensitivity compared to the control group after allergen-specific stimulation with Culicoides extract (p=0.023). In contrast, stimulation with anti-IgE induced similar amounts of released histamine in both groups (p=0.46). For further evaluation of the HRA, we prepared a receiver operating-characteristic (ROC) curve and performed a likelihood-ratio analysis for assay interpretation. Our results suggested that the assay is a valuable diagnostic tool to identify sensitization to Culicoides allergens in horses. Because some of the clinically healthy horses also showed sensitization to Culicoides extract, the assay cannot be used to distinguish allergic from non-allergic animals. The observation that sensitization is sometimes detectable in non-affected animals suggested that clinically healthy horses use immune mechanisms to control the reaction to Culicoides allergens that are different or absent in allergic horses.

Authors+Show Affiliations

Department of Population Medicine and Diagnostic Sciences, College of Veterinary Medicine, Cornell University, Ithaca, NY 14853, USA. bw73@cornell.eduNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

18926574

Citation

Wagner, Bettina, et al. "A Histamine Release Assay to Identify Sensitization to Culicoides Allergens in Horses With Skin Hypersensitivity." Veterinary Immunology and Immunopathology, vol. 126, no. 3-4, 2008, pp. 302-8.
Wagner B, Childs BA, Erb HN. A histamine release assay to identify sensitization to Culicoides allergens in horses with skin hypersensitivity. Vet Immunol Immunopathol. 2008;126(3-4):302-8.
Wagner, B., Childs, B. A., & Erb, H. N. (2008). A histamine release assay to identify sensitization to Culicoides allergens in horses with skin hypersensitivity. Veterinary Immunology and Immunopathology, 126(3-4), 302-8. https://doi.org/10.1016/j.vetimm.2008.09.001
Wagner B, Childs BA, Erb HN. A Histamine Release Assay to Identify Sensitization to Culicoides Allergens in Horses With Skin Hypersensitivity. Vet Immunol Immunopathol. 2008 Dec 15;126(3-4):302-8. PubMed PMID: 18926574.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A histamine release assay to identify sensitization to Culicoides allergens in horses with skin hypersensitivity. AU - Wagner,Bettina, AU - Childs,Bronwen A, AU - Erb,Hollis N, Y1 - 2008/09/11/ PY - 2008/07/10/received PY - 2008/08/19/revised PY - 2008/09/05/accepted PY - 2008/10/18/pubmed PY - 2009/3/4/medline PY - 2008/10/18/entrez SP - 302 EP - 8 JF - Veterinary immunology and immunopathology JO - Vet Immunol Immunopathol VL - 126 IS - 3-4 N2 - Skin hypersensitivity is an allergic disease induced in horses by allergens of Culicoides midges. The condition is typically diagnosed by clinical signs and in some horses in combination with allergy testing such as intradermal skin testing or serological allergen-specific IgE determination. Here, we describe an alternative method for allergy testing: a histamine release assay (HRA) that combines the functional aspects of skin testing with the convenience of submitting a blood sample. The assay is based on the principle that crosslinking of allergen-specific IgE bound via high-affinity IgE receptors to the surfaces of mast cells and basophils induces the release of inflammatory mediators. One of these mediators is histamine. The histamine was then detected by a colorimetric enzyme-linked immunosorbent assay. The histamine assay was used to test 33 horses with skin hypersensitivity and 20 clinically healthy control animals for histamine release from their peripheral blood basophils after stimulation with Culicoides allergen extract or monoclonal anti-IgE antibody. An increased histamine release was observed in the horses with skin hypersensitivity compared to the control group after allergen-specific stimulation with Culicoides extract (p=0.023). In contrast, stimulation with anti-IgE induced similar amounts of released histamine in both groups (p=0.46). For further evaluation of the HRA, we prepared a receiver operating-characteristic (ROC) curve and performed a likelihood-ratio analysis for assay interpretation. Our results suggested that the assay is a valuable diagnostic tool to identify sensitization to Culicoides allergens in horses. Because some of the clinically healthy horses also showed sensitization to Culicoides extract, the assay cannot be used to distinguish allergic from non-allergic animals. The observation that sensitization is sometimes detectable in non-affected animals suggested that clinically healthy horses use immune mechanisms to control the reaction to Culicoides allergens that are different or absent in allergic horses. SN - 0165-2427 UR - https://www.unboundmedicine.com/medline/citation/18926574/A_histamine_release_assay_to_identify_sensitization_to_Culicoides_allergens_in_horses_with_skin_hypersensitivity_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0165-2427(08)00329-2 DB - PRIME DP - Unbound Medicine ER -