Tags

Type your tag names separated by a space and hit enter

The core and accessory genomes of Burkholderia pseudomallei: implications for human melioidosis.
PLoS Pathog. 2008 Oct; 4(10):e1000178.PP

Abstract

Natural isolates of Burkholderia pseudomallei (Bp), the causative agent of melioidosis, can exhibit significant ecological flexibility that is likely reflective of a dynamic genome. Using whole-genome Bp microarrays, we examined patterns of gene presence and absence across 94 South East Asian strains isolated from a variety of clinical, environmental, or animal sources. 86% of the Bp K96243 reference genome was common to all the strains representing the Bp "core genome", comprising genes largely involved in essential functions (eg amino acid metabolism, protein translation). In contrast, 14% of the K96243 genome was variably present across the isolates. This Bp accessory genome encompassed multiple genomic islands (GIs), paralogous genes, and insertions/deletions, including three distinct lipopolysaccharide (LPS)-related gene clusters. Strikingly, strains recovered from cases of human melioidosis clustered on a tree based on accessory gene content, and were significantly more likely to harbor certain GIs compared to animal and environmental isolates. Consistent with the inference that the GIs may contribute to pathogenesis, experimental mutation of BPSS2053, a GI gene, reduced microbial adherence to human epithelial cells. Our results suggest that the Bp accessory genome is likely to play an important role in microbial adaptation and virulence.

Authors+Show Affiliations

Defense Medical and Environmental Research Institute, DSO National Laboratories, Singapore, Republic of Singapore.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Validation Study

Language

eng

PubMed ID

18927621

Citation

Sim, Siew Hoon, et al. "The Core and Accessory Genomes of Burkholderia Pseudomallei: Implications for Human Melioidosis." PLoS Pathogens, vol. 4, no. 10, 2008, pp. e1000178.
Sim SH, Yu Y, Lin CH, et al. The core and accessory genomes of Burkholderia pseudomallei: implications for human melioidosis. PLoS Pathog. 2008;4(10):e1000178.
Sim, S. H., Yu, Y., Lin, C. H., Karuturi, R. K., Wuthiekanun, V., Tuanyok, A., Chua, H. H., Ong, C., Paramalingam, S. S., Tan, G., Tang, L., Lau, G., Ooi, E. E., Woods, D., Feil, E., Peacock, S. J., & Tan, P. (2008). The core and accessory genomes of Burkholderia pseudomallei: implications for human melioidosis. PLoS Pathogens, 4(10), e1000178. https://doi.org/10.1371/journal.ppat.1000178
Sim SH, et al. The Core and Accessory Genomes of Burkholderia Pseudomallei: Implications for Human Melioidosis. PLoS Pathog. 2008;4(10):e1000178. PubMed PMID: 18927621.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The core and accessory genomes of Burkholderia pseudomallei: implications for human melioidosis. AU - Sim,Siew Hoon, AU - Yu,Yiting, AU - Lin,Chi Ho, AU - Karuturi,R Krishna M, AU - Wuthiekanun,Vanaporn, AU - Tuanyok,Apichai, AU - Chua,Hui Hoon, AU - Ong,Catherine, AU - Paramalingam,Sivalingam Suppiah, AU - Tan,Gladys, AU - Tang,Lynn, AU - Lau,Gary, AU - Ooi,Eng Eong, AU - Woods,Donald, AU - Feil,Edward, AU - Peacock,Sharon J, AU - Tan,Patrick, Y1 - 2008/10/17/ PY - 2008/06/23/received PY - 2008/09/16/accepted PY - 2008/10/18/pubmed PY - 2009/1/8/medline PY - 2008/10/18/entrez SP - e1000178 EP - e1000178 JF - PLoS pathogens JO - PLoS Pathog VL - 4 IS - 10 N2 - Natural isolates of Burkholderia pseudomallei (Bp), the causative agent of melioidosis, can exhibit significant ecological flexibility that is likely reflective of a dynamic genome. Using whole-genome Bp microarrays, we examined patterns of gene presence and absence across 94 South East Asian strains isolated from a variety of clinical, environmental, or animal sources. 86% of the Bp K96243 reference genome was common to all the strains representing the Bp "core genome", comprising genes largely involved in essential functions (eg amino acid metabolism, protein translation). In contrast, 14% of the K96243 genome was variably present across the isolates. This Bp accessory genome encompassed multiple genomic islands (GIs), paralogous genes, and insertions/deletions, including three distinct lipopolysaccharide (LPS)-related gene clusters. Strikingly, strains recovered from cases of human melioidosis clustered on a tree based on accessory gene content, and were significantly more likely to harbor certain GIs compared to animal and environmental isolates. Consistent with the inference that the GIs may contribute to pathogenesis, experimental mutation of BPSS2053, a GI gene, reduced microbial adherence to human epithelial cells. Our results suggest that the Bp accessory genome is likely to play an important role in microbial adaptation and virulence. SN - 1553-7374 UR - https://www.unboundmedicine.com/medline/citation/18927621/The_core_and_accessory_genomes_of_Burkholderia_pseudomallei:_implications_for_human_melioidosis_ L2 - https://dx.plos.org/10.1371/journal.ppat.1000178 DB - PRIME DP - Unbound Medicine ER -