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[Differentially expressed genes involving the correlativity between heart meridian and hypothalamus].
Zhen Ci Yan Jiu. 2008 Aug; 33(4):219-22.ZC

Abstract

OBJECTIVE

To study the bio-molecule basis of correlativity between Heart Meridian and hypothalamus from gene differential expression.

METHODS

Twenty-eight healthy Sprague-Dawley male rats were divided into 4 groups randomly: normal control, model, Heart Meridian-electroacupuncture (EA) and Lung Meridian-EA groups, with 7 cases in each. Acute myocardial ischemia (AMI) model was duplicated by occlusion of the left anterior descending branch (LAD) of the coronary artery under anesthesia (3% Nemb, 40 mg/kg). EA (1.1 mA, 2 Hz, duration of waves 300 micros) was applied to bilateral "Shenmen" (HT 7)-"Tongli" (HT 5, Heart Meridian), "Taiyuan" (LU 9)-"Lieque" (LU 7, Lung Meridian) separately for 20 min, once daily for 3 days. Then, hypothalamus tissue was taken for differentially expressed gene profiling analysis (Affymetrix GeneChip Rat Genome U 230 Affymetrix).

RESULTS

Compared with model group, among the differentially expressed genes above 2 times in Heart-Meridian-EA group and Lung-Meridian-EA group, 190 and 57 were up-regulated, 34 and 26 down-regulated separately. Compared with Lung-Meridian-EA group, among the 175 differentially expressed genes above 2 times in hypothalamus tissues of Heart-Meridian-EA group, 147 were up-regulated and 28 down-regulated.

CONCLUSION

In comparison with EA of Lung Meridian, a different gene expression profile of hypothalamus tissue is involved in the effect of EA of Heart-Meridian on acute myocardial ischemia.

Authors+Show Affiliations

Center for Researching Meridian-Viscera Correlation, Anhui College of Chinese Medicine, Hefei 230038, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

English Abstract
Journal Article

Language

chi

PubMed ID

18928111

Citation

Li, Meng, et al. "[Differentially Expressed Genes Involving the Correlativity Between Heart Meridian and Hypothalamus]." Zhen Ci Yan Jiu = Acupuncture Research, vol. 33, no. 4, 2008, pp. 219-22.
Li M, Long DH, He L, et al. [Differentially expressed genes involving the correlativity between heart meridian and hypothalamus]. Zhen Ci Yan Jiu. 2008;33(4):219-22.
Li, M., Long, D. H., He, L., Hu, L., Wang, K. M., Wu, Z. J., Cai, R. L., & Zhou, Y. P. (2008). [Differentially expressed genes involving the correlativity between heart meridian and hypothalamus]. Zhen Ci Yan Jiu = Acupuncture Research, 33(4), 219-22.
Li M, et al. [Differentially Expressed Genes Involving the Correlativity Between Heart Meridian and Hypothalamus]. Zhen Ci Yan Jiu. 2008;33(4):219-22. PubMed PMID: 18928111.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Differentially expressed genes involving the correlativity between heart meridian and hypothalamus]. AU - Li,Meng, AU - Long,Di-he, AU - He,Lu, AU - Hu,Ling, AU - Wang,Ke-ming, AU - Wu,Zi-jian, AU - Cai,Rong-lin, AU - Zhou,Yi-ping, PY - 2008/10/22/pubmed PY - 2008/11/7/medline PY - 2008/10/22/entrez SP - 219 EP - 22 JF - Zhen ci yan jiu = Acupuncture research JO - Zhen Ci Yan Jiu VL - 33 IS - 4 N2 - OBJECTIVE: To study the bio-molecule basis of correlativity between Heart Meridian and hypothalamus from gene differential expression. METHODS: Twenty-eight healthy Sprague-Dawley male rats were divided into 4 groups randomly: normal control, model, Heart Meridian-electroacupuncture (EA) and Lung Meridian-EA groups, with 7 cases in each. Acute myocardial ischemia (AMI) model was duplicated by occlusion of the left anterior descending branch (LAD) of the coronary artery under anesthesia (3% Nemb, 40 mg/kg). EA (1.1 mA, 2 Hz, duration of waves 300 micros) was applied to bilateral "Shenmen" (HT 7)-"Tongli" (HT 5, Heart Meridian), "Taiyuan" (LU 9)-"Lieque" (LU 7, Lung Meridian) separately for 20 min, once daily for 3 days. Then, hypothalamus tissue was taken for differentially expressed gene profiling analysis (Affymetrix GeneChip Rat Genome U 230 Affymetrix). RESULTS: Compared with model group, among the differentially expressed genes above 2 times in Heart-Meridian-EA group and Lung-Meridian-EA group, 190 and 57 were up-regulated, 34 and 26 down-regulated separately. Compared with Lung-Meridian-EA group, among the 175 differentially expressed genes above 2 times in hypothalamus tissues of Heart-Meridian-EA group, 147 were up-regulated and 28 down-regulated. CONCLUSION: In comparison with EA of Lung Meridian, a different gene expression profile of hypothalamus tissue is involved in the effect of EA of Heart-Meridian on acute myocardial ischemia. SN - 1000-0607 UR - https://www.unboundmedicine.com/medline/citation/18928111/[Differentially_expressed_genes_involving_the_correlativity_between_heart_meridian_and_hypothalamus]_ DB - PRIME DP - Unbound Medicine ER -