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Efficient bioethanol production from xylose by recombinant saccharomyces cerevisiae requires high activity of xylose reductase and moderate xylulokinase activity.
J Biosci Bioeng. 2008 Sep; 106(3):306-9.JB

Abstract

We varied the promoter strength of xylose reductase (XR) gene and the copy number of xylulokinase (XK) gene to determine how XR and XK activities affect the xylose-fermenting abilities of recombinant Saccharomyces cerevisiae expressing xylitol dehydrogenase (XDH). The most enhanced ethanol yield and lowered xylitol yield occurred in strain I-PGK/AUR, which has high activity of both XR and XDH and moderate XK activity.

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Authors+Show Affiliations

Matsushika A
Biomass Technology Research Center (BTRC), National Institute of Advanced Industrial Science and Technology (AIST), 2-2-2 Hirosuehiro, Kure, Hiroshima 737-0197, Japan.
Sawayama S
No affiliation info available

MeSH

Aldehyde ReductaseBioelectric Energy SourcesEnzyme ActivationEthanolGenetic EnhancementPhosphotransferases (Alcohol Group Acceptor)Recombinant ProteinsSaccharomyces cerevisiaeXylose

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18930011

Citation

Matsushika, Akinori, and Shigeki Sawayama. "Efficient Bioethanol Production From Xylose By Recombinant Saccharomyces Cerevisiae Requires High Activity of Xylose Reductase and Moderate Xylulokinase Activity." Journal of Bioscience and Bioengineering, vol. 106, no. 3, 2008, pp. 306-9.
Matsushika A, Sawayama S. Efficient bioethanol production from xylose by recombinant saccharomyces cerevisiae requires high activity of xylose reductase and moderate xylulokinase activity. J Biosci Bioeng. 2008;106(3):306-9.
Matsushika, A., & Sawayama, S. (2008). Efficient bioethanol production from xylose by recombinant saccharomyces cerevisiae requires high activity of xylose reductase and moderate xylulokinase activity. Journal of Bioscience and Bioengineering, 106(3), 306-9. https://doi.org/10.1263/jbb.106.306
Matsushika A, Sawayama S. Efficient Bioethanol Production From Xylose By Recombinant Saccharomyces Cerevisiae Requires High Activity of Xylose Reductase and Moderate Xylulokinase Activity. J Biosci Bioeng. 2008;106(3):306-9. PubMed PMID: 18930011.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Efficient bioethanol production from xylose by recombinant saccharomyces cerevisiae requires high activity of xylose reductase and moderate xylulokinase activity. AU - Matsushika,Akinori, AU - Sawayama,Shigeki, PY - 2008/04/07/received PY - 2008/06/07/accepted PY - 2008/10/22/pubmed PY - 2009/1/10/medline PY - 2008/10/22/entrez SP - 306 EP - 9 JF - Journal of bioscience and bioengineering JO - J Biosci Bioeng VL - 106 IS - 3 N2 - We varied the promoter strength of xylose reductase (XR) gene and the copy number of xylulokinase (XK) gene to determine how XR and XK activities affect the xylose-fermenting abilities of recombinant Saccharomyces cerevisiae expressing xylitol dehydrogenase (XDH). The most enhanced ethanol yield and lowered xylitol yield occurred in strain I-PGK/AUR, which has high activity of both XR and XDH and moderate XK activity. SN - 1347-4421 UR - https://www.unboundmedicine.com/medline/citation/18930011/Efficient_bioethanol_production_from_xylose_by_recombinant_saccharomyces_cerevisiae_requires_high_activity_of_xylose_reductase_and_moderate_xylulokinase_activity_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1389-1723(08)70187-7 DB - PRIME DP - Unbound Medicine ER -