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Quality evaluation of Evodia rutaecarpa (Juss.) Benth by high performance liquid chromatography with photodiode-array detection.
J Pharm Biomed Anal. 2008 Dec 01; 48(4):1230-6.JP

Abstract

A simple, sensitive and accurate HPLC-DAD method was developed for simultaneous determination of wuchuyuamide-I, quercetin, limonin, evodiamine and rutaecarpine in Evodia rutaecarpa that has been widely used as one of the traditional Chinese medicines (TCMs). Chromatographic separations were performed on a reverse-phase C(18) column with the gradient elution of acetonitrile-water and the simultaneous detection at five wavelengths. Good linear behaviors over the investigated concentration ranges were observed with the values of r higher than 0.999 for all the analytes. The recoveries measured at three levels varied from 98.77 to 102.36%. The validated method was successfully applied for the simultaneous determination of the five chemical constituents in 36 batches of samples collected from different regions or time that were investigated and authenticated as E. rutaecarpa (Juss.) Benth. Hierarchical clustering analysis (HCA) and principal components analysis (PCA) were performed to differentiate and classify the samples based on the contents of the five characteristic constituents. The total contents of evodiamine and rutaecarpine in different samples were calculated and the blending method proposed was demonstrated to be very useful in saving resources and in guiding rational herb use.

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Authors+Show Affiliations

Zhao Y
West China School of Pharmacy, Sichuan University, Chengdu 610041, China.
Li Z
No affiliation info available
Zhou X
No affiliation info available
Cai Z
No affiliation info available
Gong X
No affiliation info available
Zhou C
No affiliation info available

MeSH

CalibrationChromatography, High Pressure LiquidCluster AnalysisDrug StabilityEvodiaIndole AlkaloidsLimoninsMagnetic Resonance SpectroscopyMedicine, Chinese TraditionalMolecular StructurePlant ExtractsPrincipal Component AnalysisQuality ControlQuercetinQuinazolinesReference StandardsReproducibility of ResultsSensitivity and SpecificitySolventsSpectrometry, Mass, Electrospray IonizationSpectrophotometry, Ultraviolet

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18930617

Citation

Zhao, Yang, et al. "Quality Evaluation of Evodia Rutaecarpa (Juss.) Benth By High Performance Liquid Chromatography With Photodiode-array Detection." Journal of Pharmaceutical and Biomedical Analysis, vol. 48, no. 4, 2008, pp. 1230-6.
Zhao Y, Li Z, Zhou X, et al. Quality evaluation of Evodia rutaecarpa (Juss.) Benth by high performance liquid chromatography with photodiode-array detection. J Pharm Biomed Anal. 2008;48(4):1230-6.
Zhao, Y., Li, Z., Zhou, X., Cai, Z., Gong, X., & Zhou, C. (2008). Quality evaluation of Evodia rutaecarpa (Juss.) Benth by high performance liquid chromatography with photodiode-array detection. Journal of Pharmaceutical and Biomedical Analysis, 48(4), 1230-6. https://doi.org/10.1016/j.jpba.2008.08.035
Zhao Y, et al. Quality Evaluation of Evodia Rutaecarpa (Juss.) Benth By High Performance Liquid Chromatography With Photodiode-array Detection. J Pharm Biomed Anal. 2008 Dec 1;48(4):1230-6. PubMed PMID: 18930617.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Quality evaluation of Evodia rutaecarpa (Juss.) Benth by high performance liquid chromatography with photodiode-array detection. AU - Zhao,Yang, AU - Li,Zhangwan, AU - Zhou,Xin, AU - Cai,Zongwei, AU - Gong,Xiaojian, AU - Zhou,Chanyuan, Y1 - 2008/09/10/ PY - 2008/06/13/received PY - 2008/08/27/revised PY - 2008/08/29/accepted PY - 2008/10/22/pubmed PY - 2009/4/1/medline PY - 2008/10/22/entrez SP - 1230 EP - 6 JF - Journal of pharmaceutical and biomedical analysis JO - J Pharm Biomed Anal VL - 48 IS - 4 N2 - A simple, sensitive and accurate HPLC-DAD method was developed for simultaneous determination of wuchuyuamide-I, quercetin, limonin, evodiamine and rutaecarpine in Evodia rutaecarpa that has been widely used as one of the traditional Chinese medicines (TCMs). Chromatographic separations were performed on a reverse-phase C(18) column with the gradient elution of acetonitrile-water and the simultaneous detection at five wavelengths. Good linear behaviors over the investigated concentration ranges were observed with the values of r higher than 0.999 for all the analytes. The recoveries measured at three levels varied from 98.77 to 102.36%. The validated method was successfully applied for the simultaneous determination of the five chemical constituents in 36 batches of samples collected from different regions or time that were investigated and authenticated as E. rutaecarpa (Juss.) Benth. Hierarchical clustering analysis (HCA) and principal components analysis (PCA) were performed to differentiate and classify the samples based on the contents of the five characteristic constituents. The total contents of evodiamine and rutaecarpine in different samples were calculated and the blending method proposed was demonstrated to be very useful in saving resources and in guiding rational herb use. SN - 0731-7085 UR - https://www.unboundmedicine.com/medline/citation/18930617/Quality_evaluation_of_Evodia_rutaecarpa__Juss___Benth_by_high_performance_liquid_chromatography_with_photodiode_array_detection_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0731-7085(08)00479-2 DB - PRIME DP - Unbound Medicine ER -