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Antigenic and genetic analysis of H5 influenza viruses isolated from water birds for the purpose of vaccine use.
Arch Virol. 2008; 153(11):2041-8.AV

Abstract

In order to prepare H5N1 influenza virus vaccine, the hemagglutinins (HAs) of 14 H5 virus isolates from water birds in Asia were antigenically and genetically analyzed. Phylogenetic analysis of the H5 HA genes revealed that 13 isolates belong to Eurasian and the other one to North American lineages. Each of the deduced amino acid sequences of the HAs indicated a non-pathogenic profile. Antigenic analysis using a panel of monoclonal antibodies recognizing six different epitopes on the HA of A/duck/Pennsylvania/10218/1984 (H5N2) and chicken antiserum to an H5N1 reassortant strain generated between A/duck/Mongolia/54/2001 (H5N2) and A/duck/Mongolia/47/2001 (H7N1), [R(Dk/Mong-Dk/Mong) (H5N1)] showed that the HAs of highly pathogenic avian influenza (HPAI) viruses currently circulating in Asia were antigenically closely related to those of the present isolates from water birds. Mice subcutaneously injected with formalin-inactivated R(Dk/Mong-Dk/Mong) were protected from challenge with 100 mouse lethal dose of A/Viet Nam/1194/2004 (H5N1). The present results support the notion that the H5 isolates and the reassortant H5N1 strain should be useful for vaccine preparation.

Authors+Show Affiliations

Department of Disease Control, Graduate School of Veterinary Medicine, Hokkaido University, Kita 18 Nishi 9, Kita-ku, Sapporo, Hokkaido, 060-0818, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

18932020

Citation

Soda, Kosuke, et al. "Antigenic and Genetic Analysis of H5 Influenza Viruses Isolated From Water Birds for the Purpose of Vaccine Use." Archives of Virology, vol. 153, no. 11, 2008, pp. 2041-8.
Soda K, Ozaki H, Sakoda Y, et al. Antigenic and genetic analysis of H5 influenza viruses isolated from water birds for the purpose of vaccine use. Arch Virol. 2008;153(11):2041-8.
Soda, K., Ozaki, H., Sakoda, Y., Isoda, N., Haraguchi, Y., Sakabe, S., Kuboki, N., Kishida, N., Takada, A., & Kida, H. (2008). Antigenic and genetic analysis of H5 influenza viruses isolated from water birds for the purpose of vaccine use. Archives of Virology, 153(11), 2041-8. https://doi.org/10.1007/s00705-008-0226-3
Soda K, et al. Antigenic and Genetic Analysis of H5 Influenza Viruses Isolated From Water Birds for the Purpose of Vaccine Use. Arch Virol. 2008;153(11):2041-8. PubMed PMID: 18932020.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Antigenic and genetic analysis of H5 influenza viruses isolated from water birds for the purpose of vaccine use. AU - Soda,Kosuke, AU - Ozaki,Hiroichi, AU - Sakoda,Yoshihiro, AU - Isoda,Norikazu, AU - Haraguchi,Yoshinari, AU - Sakabe,Saori, AU - Kuboki,Noritaka, AU - Kishida,Noriko, AU - Takada,Ayato, AU - Kida,Hiroshi, Y1 - 2008/10/21/ PY - 2008/04/11/received PY - 2008/09/19/accepted PY - 2008/10/22/pubmed PY - 2008/12/17/medline PY - 2008/10/22/entrez SP - 2041 EP - 8 JF - Archives of virology JO - Arch Virol VL - 153 IS - 11 N2 - In order to prepare H5N1 influenza virus vaccine, the hemagglutinins (HAs) of 14 H5 virus isolates from water birds in Asia were antigenically and genetically analyzed. Phylogenetic analysis of the H5 HA genes revealed that 13 isolates belong to Eurasian and the other one to North American lineages. Each of the deduced amino acid sequences of the HAs indicated a non-pathogenic profile. Antigenic analysis using a panel of monoclonal antibodies recognizing six different epitopes on the HA of A/duck/Pennsylvania/10218/1984 (H5N2) and chicken antiserum to an H5N1 reassortant strain generated between A/duck/Mongolia/54/2001 (H5N2) and A/duck/Mongolia/47/2001 (H7N1), [R(Dk/Mong-Dk/Mong) (H5N1)] showed that the HAs of highly pathogenic avian influenza (HPAI) viruses currently circulating in Asia were antigenically closely related to those of the present isolates from water birds. Mice subcutaneously injected with formalin-inactivated R(Dk/Mong-Dk/Mong) were protected from challenge with 100 mouse lethal dose of A/Viet Nam/1194/2004 (H5N1). The present results support the notion that the H5 isolates and the reassortant H5N1 strain should be useful for vaccine preparation. SN - 1432-8798 UR - https://www.unboundmedicine.com/medline/citation/18932020/Antigenic_and_genetic_analysis_of_H5_influenza_viruses_isolated_from_water_birds_for_the_purpose_of_vaccine_use_ L2 - https://dx.doi.org/10.1007/s00705-008-0226-3 DB - PRIME DP - Unbound Medicine ER -