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Growth factor messenger RNA expression by human breast fibroblasts from benign and malignant lesions.
Cancer Res. 1991 Sep 15; 51(18):4978-85.CR

Abstract

Breast tumors are a complex mix of epithelial, stromal, and vascular elements. We examined primary cultures of breast fibroblasts derived from benign and malignant lesions for expression of various growth factors. All fibroblast cultures, regardless of whether they were derived from benign or malignant lesions, expressed platelet-derived growth factor A chain, basic fibroblast growth factor, fibroblast growth factor 5, and transforming growth factor beta 1 mRNA. None expressed platelet-derived growth factor B chain or transforming growth factor alpha mRNA. However, examination of mRNA expression for the insulin-like growth factors revealed that 7 of 8 fibroblasts derived from benign lesions expressed insulin-like growth factor I (IGF-I) mRNA, while only 1 of 9 fibroblasts derived from malignancies expressed IGF-I mRNA. The opposite picture was seen for insulin-like growth factor II (IGF-II) mRNA expression, in which 1 of 9 benign-derived fibroblasts expressed IGF-II mRNA, while 5 of 9 malignant-derived fibroblasts expressed IGF-II. This correlated with previous in situ hybridization data, which showed IGF-I mRNA expression confined to the stroma of benign breast tissue. PDGF treatment of tumor fibroblasts resulted in a 3-fold increase in IGF-II mRNA. Thus there was an apparent dichotomy between IGF-I mRNA expression in the majority of fibroblasts derived from benign lesions and IGF-II mRNA expression in the majority of tumor-derived fibroblasts. Since the insulin-like growth factors are potent mitogens for breast tumor epithelial cells, this further supports the notion of a paracrine growth-promoting role for the insulin-like growth factors in breast lesions and suggests that IGF-II may be the more important growth promoter in malignant lesions.

Authors+Show Affiliations

Lombardi Cancer Research Center, Georgetown University Medical Center, Washington, DC 20007.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

1893385

Citation

Cullen, K J., et al. "Growth Factor Messenger RNA Expression By Human Breast Fibroblasts From Benign and Malignant Lesions." Cancer Research, vol. 51, no. 18, 1991, pp. 4978-85.
Cullen KJ, Smith HS, Hill S, et al. Growth factor messenger RNA expression by human breast fibroblasts from benign and malignant lesions. Cancer Res. 1991;51(18):4978-85.
Cullen, K. J., Smith, H. S., Hill, S., Rosen, N., & Lippman, M. E. (1991). Growth factor messenger RNA expression by human breast fibroblasts from benign and malignant lesions. Cancer Research, 51(18), 4978-85.
Cullen KJ, et al. Growth Factor Messenger RNA Expression By Human Breast Fibroblasts From Benign and Malignant Lesions. Cancer Res. 1991 Sep 15;51(18):4978-85. PubMed PMID: 1893385.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Growth factor messenger RNA expression by human breast fibroblasts from benign and malignant lesions. AU - Cullen,K J, AU - Smith,H S, AU - Hill,S, AU - Rosen,N, AU - Lippman,M E, PY - 1991/9/15/pubmed PY - 1991/9/15/medline PY - 1991/9/15/entrez SP - 4978 EP - 85 JF - Cancer research JO - Cancer Res VL - 51 IS - 18 N2 - Breast tumors are a complex mix of epithelial, stromal, and vascular elements. We examined primary cultures of breast fibroblasts derived from benign and malignant lesions for expression of various growth factors. All fibroblast cultures, regardless of whether they were derived from benign or malignant lesions, expressed platelet-derived growth factor A chain, basic fibroblast growth factor, fibroblast growth factor 5, and transforming growth factor beta 1 mRNA. None expressed platelet-derived growth factor B chain or transforming growth factor alpha mRNA. However, examination of mRNA expression for the insulin-like growth factors revealed that 7 of 8 fibroblasts derived from benign lesions expressed insulin-like growth factor I (IGF-I) mRNA, while only 1 of 9 fibroblasts derived from malignancies expressed IGF-I mRNA. The opposite picture was seen for insulin-like growth factor II (IGF-II) mRNA expression, in which 1 of 9 benign-derived fibroblasts expressed IGF-II mRNA, while 5 of 9 malignant-derived fibroblasts expressed IGF-II. This correlated with previous in situ hybridization data, which showed IGF-I mRNA expression confined to the stroma of benign breast tissue. PDGF treatment of tumor fibroblasts resulted in a 3-fold increase in IGF-II mRNA. Thus there was an apparent dichotomy between IGF-I mRNA expression in the majority of fibroblasts derived from benign lesions and IGF-II mRNA expression in the majority of tumor-derived fibroblasts. Since the insulin-like growth factors are potent mitogens for breast tumor epithelial cells, this further supports the notion of a paracrine growth-promoting role for the insulin-like growth factors in breast lesions and suggests that IGF-II may be the more important growth promoter in malignant lesions. SN - 0008-5472 UR - https://www.unboundmedicine.com/medline/citation/1893385/Growth_factor_messenger_RNA_expression_by_human_breast_fibroblasts_from_benign_and_malignant_lesions_ L2 - http://cancerres.aacrjournals.org/cgi/pmidlookup?view=long&pmid=1893385 DB - PRIME DP - Unbound Medicine ER -