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Increased elongase and desaturase gene expression with stearidonic acid enriched diet does not enhance long-chain (n-3) content of seawater Atlantic salmon (Salmo salar L.).
J Nutr 2008; 138(11):2179-85JN

Abstract

Atlantic salmon (Salmo salar L.) can produce (n-3) long-chain (LC)-PUFA when fed biosynthetic precursors. This has potential for developing sustainable aquafeeds. Echium oil (EO) is rich in stearidonic acid [SDA; 18:4(n-3)] and bypasses the initial Delta6 desaturase (FAD6) step in the (n-3) LC-PUFA biosynthetic pathway. EO was fed to seawater Atlantic salmon for 12 wk and compared with fish fed a diet containing canola oil (CO), a source of alpha-linolenic acid [ALA; 18:3(n-3)] or fish oil (FO) that provides (n-3) LC-PUFA. Fatty acid (FA) composition of liver, white muscle, and whole fish was measured to show whether dietary precursors were endogenously biosynthesized to LC-PUFA. Gene expression of liver FA elongase and FAD5 was upregulated in EO fish compared with FO fish. Furthermore, dietary precursors affected the FA concentrations of direct biosynthetic products in all tissues. The increased gene expression in the EO fish was reflected by an increased FA concentration of eicosapentaenoic acid [20:5(n-3)] in the liver compared with the CO fish. However, the high concentrations of (n-3) LC-PUFA found in seawater Atlantic salmon fed diets rich in FO were not attained via biosynthesis from precursors (ALA or SDA) in diets.

Authors+Show Affiliations

National Centre for Marine Conservation and Resource Sustainability, University of Tasmania, Launceston, Tasmania 7250, Australia. millerm@crop.cri.nzNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18936216

Citation

Miller, Matthew R., et al. "Increased Elongase and Desaturase Gene Expression With Stearidonic Acid Enriched Diet Does Not Enhance Long-chain (n-3) Content of Seawater Atlantic Salmon (Salmo Salar L.)." The Journal of Nutrition, vol. 138, no. 11, 2008, pp. 2179-85.
Miller MR, Bridle AR, Nichols PD, et al. Increased elongase and desaturase gene expression with stearidonic acid enriched diet does not enhance long-chain (n-3) content of seawater Atlantic salmon (Salmo salar L.). J Nutr. 2008;138(11):2179-85.
Miller, M. R., Bridle, A. R., Nichols, P. D., & Carter, C. G. (2008). Increased elongase and desaturase gene expression with stearidonic acid enriched diet does not enhance long-chain (n-3) content of seawater Atlantic salmon (Salmo salar L.). The Journal of Nutrition, 138(11), pp. 2179-85. doi:10.3945/jn.108.091702.
Miller MR, et al. Increased Elongase and Desaturase Gene Expression With Stearidonic Acid Enriched Diet Does Not Enhance Long-chain (n-3) Content of Seawater Atlantic Salmon (Salmo Salar L.). J Nutr. 2008;138(11):2179-85. PubMed PMID: 18936216.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Increased elongase and desaturase gene expression with stearidonic acid enriched diet does not enhance long-chain (n-3) content of seawater Atlantic salmon (Salmo salar L.). AU - Miller,Matthew R, AU - Bridle,Andrew R, AU - Nichols,Peter D, AU - Carter,Chris G, PY - 2008/10/22/pubmed PY - 2008/12/17/medline PY - 2008/10/22/entrez SP - 2179 EP - 85 JF - The Journal of nutrition JO - J. Nutr. VL - 138 IS - 11 N2 - Atlantic salmon (Salmo salar L.) can produce (n-3) long-chain (LC)-PUFA when fed biosynthetic precursors. This has potential for developing sustainable aquafeeds. Echium oil (EO) is rich in stearidonic acid [SDA; 18:4(n-3)] and bypasses the initial Delta6 desaturase (FAD6) step in the (n-3) LC-PUFA biosynthetic pathway. EO was fed to seawater Atlantic salmon for 12 wk and compared with fish fed a diet containing canola oil (CO), a source of alpha-linolenic acid [ALA; 18:3(n-3)] or fish oil (FO) that provides (n-3) LC-PUFA. Fatty acid (FA) composition of liver, white muscle, and whole fish was measured to show whether dietary precursors were endogenously biosynthesized to LC-PUFA. Gene expression of liver FA elongase and FAD5 was upregulated in EO fish compared with FO fish. Furthermore, dietary precursors affected the FA concentrations of direct biosynthetic products in all tissues. The increased gene expression in the EO fish was reflected by an increased FA concentration of eicosapentaenoic acid [20:5(n-3)] in the liver compared with the CO fish. However, the high concentrations of (n-3) LC-PUFA found in seawater Atlantic salmon fed diets rich in FO were not attained via biosynthesis from precursors (ALA or SDA) in diets. SN - 1541-6100 UR - https://www.unboundmedicine.com/medline/citation/18936216/Increased_elongase_and_desaturase_gene_expression_with_stearidonic_acid_enriched_diet_does_not_enhance_long_chain__n_3__content_of_seawater_Atlantic_salmon__Salmo_salar_L___ L2 - https://academic.oup.com/jn/article-lookup/doi/10.3945/jn.108.091702 DB - PRIME DP - Unbound Medicine ER -