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Electrochemical biosensor for detection of adenosine based on structure-switching aptamer and amplification with reporter probe DNA modified Au nanoparticles.
Anal Chem. 2008 Nov 15; 80(22):8382-8.AC

Abstract

In the present study, an electrochemical sensing strategy for highly sensitive detection of small molecules was developed based on switching structures of aptamers from DNA/DNA duplex to DNA/target complex. A gold electrode was first modified with gold nanoparticles (AuNPs), and thiolated capture probe was immobilized onto the electrode via sulfur-gold affinity. Then, a "sandwich-type" strategy was employed, which involved a linker DNA containing antiadenosine aptamer sequence and reporter DNA loaded on AuNPs. In the presence of adenosine, the aptamer part bound with adenosine and folded to the complex structure. As a result, the reporter probes together with AuNPs were released into solution and reduced a decrease in peak current. With the enhancement effect of AuNPs, a detection limit as low as 1.8 x 10(-10) M for adenosine was achieved. The sensor exhibited excellent selectivity against other nucleosides and could be used to detect adenosine from real human serum samples.

Authors+Show Affiliations

Key Laboratory of Eco-chemical Engineering, Ministry of Education, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042, China. shushzhang@126.comNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18939854

Citation

Zhang, Shusheng, et al. "Electrochemical Biosensor for Detection of Adenosine Based On Structure-switching Aptamer and Amplification With Reporter Probe DNA Modified Au Nanoparticles." Analytical Chemistry, vol. 80, no. 22, 2008, pp. 8382-8.
Zhang S, Xia J, Li X. Electrochemical biosensor for detection of adenosine based on structure-switching aptamer and amplification with reporter probe DNA modified Au nanoparticles. Anal Chem. 2008;80(22):8382-8.
Zhang, S., Xia, J., & Li, X. (2008). Electrochemical biosensor for detection of adenosine based on structure-switching aptamer and amplification with reporter probe DNA modified Au nanoparticles. Analytical Chemistry, 80(22), 8382-8. https://doi.org/10.1021/ac800857p
Zhang S, Xia J, Li X. Electrochemical Biosensor for Detection of Adenosine Based On Structure-switching Aptamer and Amplification With Reporter Probe DNA Modified Au Nanoparticles. Anal Chem. 2008 Nov 15;80(22):8382-8. PubMed PMID: 18939854.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Electrochemical biosensor for detection of adenosine based on structure-switching aptamer and amplification with reporter probe DNA modified Au nanoparticles. AU - Zhang,Shusheng, AU - Xia,Jianping, AU - Li,Xuemei, Y1 - 2008/10/22/ PY - 2008/10/23/pubmed PY - 2009/2/3/medline PY - 2008/10/23/entrez SP - 8382 EP - 8 JF - Analytical chemistry JO - Anal Chem VL - 80 IS - 22 N2 - In the present study, an electrochemical sensing strategy for highly sensitive detection of small molecules was developed based on switching structures of aptamers from DNA/DNA duplex to DNA/target complex. A gold electrode was first modified with gold nanoparticles (AuNPs), and thiolated capture probe was immobilized onto the electrode via sulfur-gold affinity. Then, a "sandwich-type" strategy was employed, which involved a linker DNA containing antiadenosine aptamer sequence and reporter DNA loaded on AuNPs. In the presence of adenosine, the aptamer part bound with adenosine and folded to the complex structure. As a result, the reporter probes together with AuNPs were released into solution and reduced a decrease in peak current. With the enhancement effect of AuNPs, a detection limit as low as 1.8 x 10(-10) M for adenosine was achieved. The sensor exhibited excellent selectivity against other nucleosides and could be used to detect adenosine from real human serum samples. SN - 1520-6882 UR - https://www.unboundmedicine.com/medline/citation/18939854/Electrochemical_biosensor_for_detection_of_adenosine_based_on_structure_switching_aptamer_and_amplification_with_reporter_probe_DNA_modified_Au_nanoparticles_ L2 - https://doi.org/10.1021/ac800857p DB - PRIME DP - Unbound Medicine ER -