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Two adjacent nucleotide-binding site-leucine-rich repeat class genes are required to confer Pikm-specific rice blast resistance.
Genetics. 2008 Dec; 180(4):2267-76.G

Abstract

The rice blast resistance gene Pikm was cloned by a map-based cloning strategy. High-resolution genetic mapping and sequencing of the gene region in the Pikm-containing cultivar Tsuyuake narrowed down the candidate region to a 131-kb genomic interval. Sequence analysis predicted two adjacently arranged resistance-like genes, Pikm1-TS and Pikm2-TS, within this candidate region. These genes encoded proteins with a nucleotide-binding site (NBS) and leucine-rich repeats (LRRs) and were considered the most probable candidates for Pikm. However, genetic complementation analysis of transgenic lines individually carrying these two genes negated the possibility that either Pikm1-TS or Pikm2-TS alone was Pikm. Instead, it was revealed that transgenic lines carrying both of these genes expressed blast resistance. The results of the complementation analysis and an evaluation of the resistance specificity of the transgenic lines to blast isolates demonstrated that Pikm-specific resistance is conferred by cooperation of Pikm1-TS and Pikm2-TS. Although these two genes are not homologous with each other, they both contain all the conserved motifs necessary for an NBS-LRR class gene to function independently as a resistance gene.

Authors+Show Affiliations

QTL Genomics Research Center, National Institute of Agrobiological Sciences, Tsukuba, Ibaraki 305-8602, Japan. ashikawa@affrc.go.jpNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18940787

Citation

Ashikawa, Ikuo, et al. "Two Adjacent Nucleotide-binding Site-leucine-rich Repeat Class Genes Are Required to Confer Pikm-specific Rice Blast Resistance." Genetics, vol. 180, no. 4, 2008, pp. 2267-76.
Ashikawa I, Hayashi N, Yamane H, et al. Two adjacent nucleotide-binding site-leucine-rich repeat class genes are required to confer Pikm-specific rice blast resistance. Genetics. 2008;180(4):2267-76.
Ashikawa, I., Hayashi, N., Yamane, H., Kanamori, H., Wu, J., Matsumoto, T., Ono, K., & Yano, M. (2008). Two adjacent nucleotide-binding site-leucine-rich repeat class genes are required to confer Pikm-specific rice blast resistance. Genetics, 180(4), 2267-76. https://doi.org/10.1534/genetics.108.095034
Ashikawa I, et al. Two Adjacent Nucleotide-binding Site-leucine-rich Repeat Class Genes Are Required to Confer Pikm-specific Rice Blast Resistance. Genetics. 2008;180(4):2267-76. PubMed PMID: 18940787.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Two adjacent nucleotide-binding site-leucine-rich repeat class genes are required to confer Pikm-specific rice blast resistance. AU - Ashikawa,Ikuo, AU - Hayashi,Nagao, AU - Yamane,Hiroko, AU - Kanamori,Hiroyuki, AU - Wu,Jianzhong, AU - Matsumoto,Takashi, AU - Ono,Kazuko, AU - Yano,Masahiro, Y1 - 2008/10/20/ PY - 2008/10/23/entrez PY - 2008/10/23/pubmed PY - 2009/3/28/medline SP - 2267 EP - 76 JF - Genetics JO - Genetics VL - 180 IS - 4 N2 - The rice blast resistance gene Pikm was cloned by a map-based cloning strategy. High-resolution genetic mapping and sequencing of the gene region in the Pikm-containing cultivar Tsuyuake narrowed down the candidate region to a 131-kb genomic interval. Sequence analysis predicted two adjacently arranged resistance-like genes, Pikm1-TS and Pikm2-TS, within this candidate region. These genes encoded proteins with a nucleotide-binding site (NBS) and leucine-rich repeats (LRRs) and were considered the most probable candidates for Pikm. However, genetic complementation analysis of transgenic lines individually carrying these two genes negated the possibility that either Pikm1-TS or Pikm2-TS alone was Pikm. Instead, it was revealed that transgenic lines carrying both of these genes expressed blast resistance. The results of the complementation analysis and an evaluation of the resistance specificity of the transgenic lines to blast isolates demonstrated that Pikm-specific resistance is conferred by cooperation of Pikm1-TS and Pikm2-TS. Although these two genes are not homologous with each other, they both contain all the conserved motifs necessary for an NBS-LRR class gene to function independently as a resistance gene. SN - 0016-6731 UR - https://www.unboundmedicine.com/medline/citation/18940787/Two_adjacent_nucleotide_binding_site_leucine_rich_repeat_class_genes_are_required_to_confer_Pikm_specific_rice_blast_resistance_ L2 - http://www.genetics.org/cgi/pmidlookup?view=long&pmid=18940787 DB - PRIME DP - Unbound Medicine ER -