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Electrochemical nitrite biosensor based on the immobilization of hemoglobin on an electrode modified by multiwall carbon nanotubes and positively charged gold nanoparticle.
Bioprocess Biosyst Eng. 2009 Jun; 32(4):485-92.BB

Abstract

The report is on an electrochemical biosensor with remarkably improved sensitivity toward nitrite. In this strategy, positively charged gold nanoparticle (PCNA) is used in combination with multiwall carbon nanotubes (MWCNT) by electrostatic adsorption for fabricating PCNA/MWCNT films. Then hemoglobin (Hb) biocatalyst will easily be attached to the surface of the combination films aforementioned. After that, the Hb/PCNA films are immobilized onto the Hb/PCNA/MWCNT films through layer-by-layer assembly technique. The (Hb/PCNA)(2)/MWNT/GC electrode thus prepared exhibits enhanced electrocatalytic behavior to the reduction of nitrite at -0.10 V versus SCE in 0.05 M H(2)SO(4) solution. On condition of the low detecting potential and low pH, interference caused by direct electrochemical oxidation or oxidizable substances can be prevented. Therefore, the modified electrode shows fast response time, very high sensitivity, good selectivity and stability. The current response of the sensor increases linearly with nitrite concentration from a range of 3.6 x 10(-6) to 3.0 x 10(-3) M with a detection limit(S /N = 3) of 9.6 x 10(-7) M.

Authors+Show Affiliations

Forensic Identification Center, College of Criminal Investigation, Southwest University of Political Science and Law, 401120, Chongqing, China. zly100@126.comNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18941796

Citation

Zhang, Lingyan, and Min Yi. "Electrochemical Nitrite Biosensor Based On the Immobilization of Hemoglobin On an Electrode Modified By Multiwall Carbon Nanotubes and Positively Charged Gold Nanoparticle." Bioprocess and Biosystems Engineering, vol. 32, no. 4, 2009, pp. 485-92.
Zhang L, Yi M. Electrochemical nitrite biosensor based on the immobilization of hemoglobin on an electrode modified by multiwall carbon nanotubes and positively charged gold nanoparticle. Bioprocess Biosyst Eng. 2009;32(4):485-92.
Zhang, L., & Yi, M. (2009). Electrochemical nitrite biosensor based on the immobilization of hemoglobin on an electrode modified by multiwall carbon nanotubes and positively charged gold nanoparticle. Bioprocess and Biosystems Engineering, 32(4), 485-92. https://doi.org/10.1007/s00449-008-0268-7
Zhang L, Yi M. Electrochemical Nitrite Biosensor Based On the Immobilization of Hemoglobin On an Electrode Modified By Multiwall Carbon Nanotubes and Positively Charged Gold Nanoparticle. Bioprocess Biosyst Eng. 2009;32(4):485-92. PubMed PMID: 18941796.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Electrochemical nitrite biosensor based on the immobilization of hemoglobin on an electrode modified by multiwall carbon nanotubes and positively charged gold nanoparticle. AU - Zhang,Lingyan, AU - Yi,Min, Y1 - 2008/10/22/ PY - 2008/02/04/received PY - 2008/09/26/accepted PY - 2008/10/23/pubmed PY - 2009/8/11/medline PY - 2008/10/23/entrez SP - 485 EP - 92 JF - Bioprocess and biosystems engineering JO - Bioprocess Biosyst Eng VL - 32 IS - 4 N2 - The report is on an electrochemical biosensor with remarkably improved sensitivity toward nitrite. In this strategy, positively charged gold nanoparticle (PCNA) is used in combination with multiwall carbon nanotubes (MWCNT) by electrostatic adsorption for fabricating PCNA/MWCNT films. Then hemoglobin (Hb) biocatalyst will easily be attached to the surface of the combination films aforementioned. After that, the Hb/PCNA films are immobilized onto the Hb/PCNA/MWCNT films through layer-by-layer assembly technique. The (Hb/PCNA)(2)/MWNT/GC electrode thus prepared exhibits enhanced electrocatalytic behavior to the reduction of nitrite at -0.10 V versus SCE in 0.05 M H(2)SO(4) solution. On condition of the low detecting potential and low pH, interference caused by direct electrochemical oxidation or oxidizable substances can be prevented. Therefore, the modified electrode shows fast response time, very high sensitivity, good selectivity and stability. The current response of the sensor increases linearly with nitrite concentration from a range of 3.6 x 10(-6) to 3.0 x 10(-3) M with a detection limit(S /N = 3) of 9.6 x 10(-7) M. SN - 1615-7605 UR - https://www.unboundmedicine.com/medline/citation/18941796/Electrochemical_nitrite_biosensor_based_on_the_immobilization_of_hemoglobin_on_an_electrode_modified_by_multiwall_carbon_nanotubes_and_positively_charged_gold_nanoparticle_ L2 - https://dx.doi.org/10.1007/s00449-008-0268-7 DB - PRIME DP - Unbound Medicine ER -