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Determination of amphetamine, methamphetamine, MDA and MDMA in human hair by GC-EI-MS after derivatization with perfluorooctanoyl chloride.
Scand J Clin Lab Invest. 2009; 69(1):113-20.SJ

Abstract

The aim of this study was to develop a quantitative gas chromatography mass spectrometry (GC-MS) method to determine the classical amphetamines and their methylenedioxylated derivatives in human hair. The procedure involved liquid-liquid extraction of hydrolysed hair spiked with deuterated internal standards and direct derivatization with perfluorooctanoyl chloride. After evaporation of the organic phase and dissolution in butylacetate, the derivatized compounds were injected into a GC-MS. Method validation results showed a linear range from 0.25 to 25 ng/mg for the target compounds: amphetamine (AM), methamphetamine (MA), methylenedioxyamphetamine (MDA) and methylenedioxymethamphetamine (MDMA or ecstasy). An intra-day precision of 3-6% RSD and an inter-day precision of 3-17% RSD were observed. Trueness was between 96 % and 106% for the target compounds. The limit of detection ranged from 0.07 to 0.14 ng/mg and of quantification from 0.24 to 0.46 ng/mg, depending on compound. The method was applied on 40 authentic hair samples (segmented or pooled hair), of which 15 cases involved amphetamine and/or ecstasy. The hair concentrations ranged from LOD to 3.2 ng/mg of AM in 7 cases, to 0.4 ng/mg of MDA in 3 cases and to 5.9 ng/mg of MDMA in 13 cases. MA was only detected once at trace level. The method, including the derivatization procedure, is simple and robust with a sensitivity that is satisfactory for measurement of amphetamines and ecstasy in hair from abusers.

Authors+Show Affiliations

Section of Forensic Chemistry, Department of Forensic Medicine, Faculty of Health Sciences, University of Copenhagen, Denmark. ssj@forensic.ku.dkNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

18942018

Citation

Johansen, Sys Stybe, and Jakob Jornil. "Determination of Amphetamine, Methamphetamine, MDA and MDMA in Human Hair By GC-EI-MS After Derivatization With Perfluorooctanoyl Chloride." Scandinavian Journal of Clinical and Laboratory Investigation, vol. 69, no. 1, 2009, pp. 113-20.
Johansen SS, Jornil J. Determination of amphetamine, methamphetamine, MDA and MDMA in human hair by GC-EI-MS after derivatization with perfluorooctanoyl chloride. Scand J Clin Lab Invest. 2009;69(1):113-20.
Johansen, S. S., & Jornil, J. (2009). Determination of amphetamine, methamphetamine, MDA and MDMA in human hair by GC-EI-MS after derivatization with perfluorooctanoyl chloride. Scandinavian Journal of Clinical and Laboratory Investigation, 69(1), 113-20. https://doi.org/10.1080/00365510802439072
Johansen SS, Jornil J. Determination of Amphetamine, Methamphetamine, MDA and MDMA in Human Hair By GC-EI-MS After Derivatization With Perfluorooctanoyl Chloride. Scand J Clin Lab Invest. 2009;69(1):113-20. PubMed PMID: 18942018.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Determination of amphetamine, methamphetamine, MDA and MDMA in human hair by GC-EI-MS after derivatization with perfluorooctanoyl chloride. AU - Johansen,Sys Stybe, AU - Jornil,Jakob, PY - 2008/10/23/pubmed PY - 2009/3/21/medline PY - 2008/10/23/entrez SP - 113 EP - 20 JF - Scandinavian journal of clinical and laboratory investigation JO - Scand. J. Clin. Lab. Invest. VL - 69 IS - 1 N2 - The aim of this study was to develop a quantitative gas chromatography mass spectrometry (GC-MS) method to determine the classical amphetamines and their methylenedioxylated derivatives in human hair. The procedure involved liquid-liquid extraction of hydrolysed hair spiked with deuterated internal standards and direct derivatization with perfluorooctanoyl chloride. After evaporation of the organic phase and dissolution in butylacetate, the derivatized compounds were injected into a GC-MS. Method validation results showed a linear range from 0.25 to 25 ng/mg for the target compounds: amphetamine (AM), methamphetamine (MA), methylenedioxyamphetamine (MDA) and methylenedioxymethamphetamine (MDMA or ecstasy). An intra-day precision of 3-6% RSD and an inter-day precision of 3-17% RSD were observed. Trueness was between 96 % and 106% for the target compounds. The limit of detection ranged from 0.07 to 0.14 ng/mg and of quantification from 0.24 to 0.46 ng/mg, depending on compound. The method was applied on 40 authentic hair samples (segmented or pooled hair), of which 15 cases involved amphetamine and/or ecstasy. The hair concentrations ranged from LOD to 3.2 ng/mg of AM in 7 cases, to 0.4 ng/mg of MDA in 3 cases and to 5.9 ng/mg of MDMA in 13 cases. MA was only detected once at trace level. The method, including the derivatization procedure, is simple and robust with a sensitivity that is satisfactory for measurement of amphetamines and ecstasy in hair from abusers. SN - 1502-7686 UR - https://www.unboundmedicine.com/medline/citation/18942018/Determination_of_amphetamine_methamphetamine_MDA_and_MDMA_in_human_hair_by_GC_EI_MS_after_derivatization_with_perfluorooctanoyl_chloride_ L2 - http://www.tandfonline.com/doi/full/10.1080/00365510802439072 DB - PRIME DP - Unbound Medicine ER -