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Proteomic analysis of the neuroprotective mechanisms of acupuncture treatment in a Parkinson's disease mouse model.
Proteomics. 2008 Nov; 8(22):4822-32.P

Abstract

Acupuncture is frequently used as an alternative therapy for Parkinson's disease (PD), and it attenuates dopaminergic (DA) neurodegeneration in the substantia nigra (SN) in PD animal models. Using proteomic analysis, we investigated whether acupuncture alters protein expression in the SN to favor attenuation of neuronal degeneration. In C57BL/6 mice treated with 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP, 30 mg/kg/day), intraperitoneal (i.p.) for 5 days, 2 or 100 Hz electroacupuncture (EA) was applied at the effective and specific acupoint, GB34, once a day for 12 consecutive days from the first MPTP treatment. Both treatments in MPTP mice led to restoration of behavioral impairment and rescued tyrosine hydroxylase (TH)-positive DA neurodegeneration. Using peptide fingerprinting MS, we identified changes in 22 proteins in the SN following MPTP treatment, and nine of these proteins were normalized by EA. They were involved in cell death regulation, inflammation, or restoration from damage. The levels of cyclophilin A (CypA), which is a neuroprotective agent, were unchanged by MPTP treatment but were increased in MPTP-EA mice. These results suggest that acupoint GB34-specific EA changes protein expression profiles in the SN in favor of DA neuronal survival in MPTP-treated mice, and that EA treatment may be an effective therapy for PD patients.

Authors+Show Affiliations

Medical Science Research Center, Dongguk University, Gyeonggi-Do, Republic of Korea.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18942673

Citation

Jeon, Songhee, et al. "Proteomic Analysis of the Neuroprotective Mechanisms of Acupuncture Treatment in a Parkinson's Disease Mouse Model." Proteomics, vol. 8, no. 22, 2008, pp. 4822-32.
Jeon S, Kim YJ, Kim ST, et al. Proteomic analysis of the neuroprotective mechanisms of acupuncture treatment in a Parkinson's disease mouse model. Proteomics. 2008;8(22):4822-32.
Jeon, S., Kim, Y. J., Kim, S. T., Moon, W., Chae, Y., Kang, M., Chung, M. Y., Lee, H., Hong, M. S., Chung, J. H., Joh, T. H., Lee, H., & Park, H. J. (2008). Proteomic analysis of the neuroprotective mechanisms of acupuncture treatment in a Parkinson's disease mouse model. Proteomics, 8(22), 4822-32. https://doi.org/10.1002/pmic.200700955
Jeon S, et al. Proteomic Analysis of the Neuroprotective Mechanisms of Acupuncture Treatment in a Parkinson's Disease Mouse Model. Proteomics. 2008;8(22):4822-32. PubMed PMID: 18942673.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Proteomic analysis of the neuroprotective mechanisms of acupuncture treatment in a Parkinson's disease mouse model. AU - Jeon,Songhee, AU - Kim,Youn Jung, AU - Kim,Seung-Tae, AU - Moon,Woongjoon, AU - Chae,Younbyoung, AU - Kang,Minjeong, AU - Chung,Mi-Young, AU - Lee,Hyangsook, AU - Hong,Mi-Sook, AU - Chung,Joo-Ho, AU - Joh,Tong H, AU - Lee,Hyejung, AU - Park,Hi-Joon, PY - 2008/10/24/pubmed PY - 2009/1/14/medline PY - 2008/10/24/entrez SP - 4822 EP - 32 JF - Proteomics JO - Proteomics VL - 8 IS - 22 N2 - Acupuncture is frequently used as an alternative therapy for Parkinson's disease (PD), and it attenuates dopaminergic (DA) neurodegeneration in the substantia nigra (SN) in PD animal models. Using proteomic analysis, we investigated whether acupuncture alters protein expression in the SN to favor attenuation of neuronal degeneration. In C57BL/6 mice treated with 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP, 30 mg/kg/day), intraperitoneal (i.p.) for 5 days, 2 or 100 Hz electroacupuncture (EA) was applied at the effective and specific acupoint, GB34, once a day for 12 consecutive days from the first MPTP treatment. Both treatments in MPTP mice led to restoration of behavioral impairment and rescued tyrosine hydroxylase (TH)-positive DA neurodegeneration. Using peptide fingerprinting MS, we identified changes in 22 proteins in the SN following MPTP treatment, and nine of these proteins were normalized by EA. They were involved in cell death regulation, inflammation, or restoration from damage. The levels of cyclophilin A (CypA), which is a neuroprotective agent, were unchanged by MPTP treatment but were increased in MPTP-EA mice. These results suggest that acupoint GB34-specific EA changes protein expression profiles in the SN in favor of DA neuronal survival in MPTP-treated mice, and that EA treatment may be an effective therapy for PD patients. SN - 1615-9861 UR - https://www.unboundmedicine.com/medline/citation/18942673/Proteomic_analysis_of_the_neuroprotective_mechanisms_of_acupuncture_treatment_in_a_Parkinson's_disease_mouse_model_ L2 - https://doi.org/10.1002/pmic.200700955 DB - PRIME DP - Unbound Medicine ER -