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Subcuticular-Intracellular Hemibiotrophic and Intercellular Necrotrophic Development of Colletotrichum acutatum on Almond.
Phytopathology 2005; 95(7):751-8P

Abstract

ABSTRACT

The early infection and colonization processes of Colletotrichum acutatum on leaves and petals of two almond cultivars with different susceptibility to anthracnose (i.e., cvs. Carmel and Nonpareil) were examined using digital image analysis of light micrographs and histological techniques. Inoculated tissue surfaces were evaluated at selected times after inoculation and incubation at 20 degrees C. Depth maps and line profiles of the digital image analysis allowed rapid depth quantification of fungal colonization in numerous tissue samples. The results showed that the early development of C. acutatum on petals was different from that on leaf tissue. On petals, conidia germinated more rapidly, germ tubes were longer, and fewer appressoria developed than on leaves. On both tissues, penetration by the pathogen occurred from appressoria and host colonization was first subcuticular and then intracellular. On petals, colonizing hyphae were first observed 24 h after inoculation and incubation at 20 degrees C, whereas on leaves they were seen 48 to 72 h after inoculation. Intercellular hyphae were formed before host cells became necrotic and macroscopic lesions developed on petals >/=48 h and on leaves >/=96 h after inoculation. Histological studies complemented data obtained by digital image analysis and showed that the fungus produced infection vesicles and broad hyphae below the cuticle and in epidermal cells. In both tissues, during the first 24 to 48 h after penetration fungal colonization was biotrophic based on the presence of healthy host cells adjacent to fungal hyphae. Later, during intercellular growth, the host-pathogen interaction became necrotrophic with collapsed host cells. Quantitative differences in appressorium formation and host colonization were found between the two almond cultivars studied. Thus, on the less susceptible cv. Nonpareil fewer appressoria developed and host colonization was reduced compared with that on cv. Carmel.

Authors

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Pub Type(s)

Journal Article

Language

eng

PubMed ID

18943006

Citation

Diéguez-Uribeondo, J, et al. "Subcuticular-Intracellular Hemibiotrophic and Intercellular Necrotrophic Development of Colletotrichum Acutatum On Almond." Phytopathology, vol. 95, no. 7, 2005, pp. 751-8.
Diéguez-Uribeondo J, Förster H, Soto-Estrada A, et al. Subcuticular-Intracellular Hemibiotrophic and Intercellular Necrotrophic Development of Colletotrichum acutatum on Almond. Phytopathology. 2005;95(7):751-8.
Diéguez-Uribeondo, J., Förster, H., Soto-Estrada, A., & Adaskaveg, J. E. (2005). Subcuticular-Intracellular Hemibiotrophic and Intercellular Necrotrophic Development of Colletotrichum acutatum on Almond. Phytopathology, 95(7), pp. 751-8. doi:10.1094/PHYTO-95-0751.
Diéguez-Uribeondo J, et al. Subcuticular-Intracellular Hemibiotrophic and Intercellular Necrotrophic Development of Colletotrichum Acutatum On Almond. Phytopathology. 2005;95(7):751-8. PubMed PMID: 18943006.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Subcuticular-Intracellular Hemibiotrophic and Intercellular Necrotrophic Development of Colletotrichum acutatum on Almond. AU - Diéguez-Uribeondo,J, AU - Förster,H, AU - Soto-Estrada,A, AU - Adaskaveg,J E, PY - 2008/10/24/pubmed PY - 2008/10/24/medline PY - 2008/10/24/entrez SP - 751 EP - 8 JF - Phytopathology JO - Phytopathology VL - 95 IS - 7 N2 - ABSTRACT The early infection and colonization processes of Colletotrichum acutatum on leaves and petals of two almond cultivars with different susceptibility to anthracnose (i.e., cvs. Carmel and Nonpareil) were examined using digital image analysis of light micrographs and histological techniques. Inoculated tissue surfaces were evaluated at selected times after inoculation and incubation at 20 degrees C. Depth maps and line profiles of the digital image analysis allowed rapid depth quantification of fungal colonization in numerous tissue samples. The results showed that the early development of C. acutatum on petals was different from that on leaf tissue. On petals, conidia germinated more rapidly, germ tubes were longer, and fewer appressoria developed than on leaves. On both tissues, penetration by the pathogen occurred from appressoria and host colonization was first subcuticular and then intracellular. On petals, colonizing hyphae were first observed 24 h after inoculation and incubation at 20 degrees C, whereas on leaves they were seen 48 to 72 h after inoculation. Intercellular hyphae were formed before host cells became necrotic and macroscopic lesions developed on petals >/=48 h and on leaves >/=96 h after inoculation. Histological studies complemented data obtained by digital image analysis and showed that the fungus produced infection vesicles and broad hyphae below the cuticle and in epidermal cells. In both tissues, during the first 24 to 48 h after penetration fungal colonization was biotrophic based on the presence of healthy host cells adjacent to fungal hyphae. Later, during intercellular growth, the host-pathogen interaction became necrotrophic with collapsed host cells. Quantitative differences in appressorium formation and host colonization were found between the two almond cultivars studied. Thus, on the less susceptible cv. Nonpareil fewer appressoria developed and host colonization was reduced compared with that on cv. Carmel. SN - 0031-949X UR - https://www.unboundmedicine.com/medline/citation/18943006/Subcuticular_Intracellular_Hemibiotrophic_and_Intercellular_Necrotrophic_Development_of_Colletotrichum_acutatum_on_Almond_ L2 - http://apsjournals.apsnet.org/doi/full/10.1094/PHYTO-95-0751?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -