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Identification of quantitative trait loci for resistance against Verticillium longisporum in oilseed rape (Brassica napus).
Phytopathology. 2008 Feb; 98(2):215-21.P

Abstract

Verticillium longisporum is one of the major pathogens of oilseed rape (Brassica napus; genome AACC, 2n = 38) in Europe. Current European cultivars possess only a low level of resistance against V. longisporum, meaning that heavy infection can cause major yield losses. The aim of this study was to identify quantitative trait loci (QTL) for resistance against V. longisporum as a starting point for marker-assisted breeding of resistant cultivars. Resistance QTL were localized in a segregating oilseed rape population of 163 doubled haploid (DH) lines derived by microspore culture from the F1 of a cross between two B. napus breeding lines, one of which exhibited V. longisporum resistance derived by pedigree selection from a resynthesized B. napus genotype. A genetic map was constructed comprising 165 restriction fragment length polymorphism, 94 amplified fragment length polymorphism and 45 simple sequence repeats (SSR) markers covering a total of 1,739 cM on 19 linkage groups. Seedlings of the DH lines and parents were inoculated with V. longisporum isolates in four greenhouse experiments performed in Sweden during autumn 1999. In three of the experiments the DH lines were inoculated with a mixture of five isolates, while in the fourth experiment only one of the isolates was used. The intention was to simulate four different environments with variable disease pressure, while still maintaining uniform conditions in each environment to enable reliable disease scoring. The disease index (DI) was calculated by scoring symptoms on a total of 21 inoculated plants per line in comparison to 21 noninoculated plants per line. Using the composite interval mapping procedure a total of four different chromosome regions could be identified that showed significant QTL for resistance in more than one environment. Two major QTL regions were identified on the C-genome linkage groups N14 and N15, respectively; each of these QTL consistently exhibited significant effects on resistance in multiple environments. The presence of flanking markers for the respective QTL was associated with a significant reduction in DI in the inoculated DH lines.

Authors+Show Affiliations

Department of Plant Breeding, Research Centre for BioSystems, Land Use and Nutrition, Justus Liebig University, Giessen, Germany.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18943198

Citation

Rygulla, W, et al. "Identification of Quantitative Trait Loci for Resistance Against Verticillium Longisporum in Oilseed Rape (Brassica Napus)." Phytopathology, vol. 98, no. 2, 2008, pp. 215-21.
Rygulla W, Snowdon RJ, Friedt W, et al. Identification of quantitative trait loci for resistance against Verticillium longisporum in oilseed rape (Brassica napus). Phytopathology. 2008;98(2):215-21.
Rygulla, W., Snowdon, R. J., Friedt, W., Happstadius, I., Cheung, W. Y., & Chen, D. (2008). Identification of quantitative trait loci for resistance against Verticillium longisporum in oilseed rape (Brassica napus). Phytopathology, 98(2), 215-21. https://doi.org/10.1094/PHYTO-98-2-0215
Rygulla W, et al. Identification of Quantitative Trait Loci for Resistance Against Verticillium Longisporum in Oilseed Rape (Brassica Napus). Phytopathology. 2008;98(2):215-21. PubMed PMID: 18943198.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Identification of quantitative trait loci for resistance against Verticillium longisporum in oilseed rape (Brassica napus). AU - Rygulla,W, AU - Snowdon,R J, AU - Friedt,W, AU - Happstadius,I, AU - Cheung,W Y, AU - Chen,D, PY - 2008/10/24/pubmed PY - 2009/3/20/medline PY - 2008/10/24/entrez SP - 215 EP - 21 JF - Phytopathology JO - Phytopathology VL - 98 IS - 2 N2 - Verticillium longisporum is one of the major pathogens of oilseed rape (Brassica napus; genome AACC, 2n = 38) in Europe. Current European cultivars possess only a low level of resistance against V. longisporum, meaning that heavy infection can cause major yield losses. The aim of this study was to identify quantitative trait loci (QTL) for resistance against V. longisporum as a starting point for marker-assisted breeding of resistant cultivars. Resistance QTL were localized in a segregating oilseed rape population of 163 doubled haploid (DH) lines derived by microspore culture from the F1 of a cross between two B. napus breeding lines, one of which exhibited V. longisporum resistance derived by pedigree selection from a resynthesized B. napus genotype. A genetic map was constructed comprising 165 restriction fragment length polymorphism, 94 amplified fragment length polymorphism and 45 simple sequence repeats (SSR) markers covering a total of 1,739 cM on 19 linkage groups. Seedlings of the DH lines and parents were inoculated with V. longisporum isolates in four greenhouse experiments performed in Sweden during autumn 1999. In three of the experiments the DH lines were inoculated with a mixture of five isolates, while in the fourth experiment only one of the isolates was used. The intention was to simulate four different environments with variable disease pressure, while still maintaining uniform conditions in each environment to enable reliable disease scoring. The disease index (DI) was calculated by scoring symptoms on a total of 21 inoculated plants per line in comparison to 21 noninoculated plants per line. Using the composite interval mapping procedure a total of four different chromosome regions could be identified that showed significant QTL for resistance in more than one environment. Two major QTL regions were identified on the C-genome linkage groups N14 and N15, respectively; each of these QTL consistently exhibited significant effects on resistance in multiple environments. The presence of flanking markers for the respective QTL was associated with a significant reduction in DI in the inoculated DH lines. SN - 0031-949X UR - https://www.unboundmedicine.com/medline/citation/18943198/Identification_of_quantitative_trait_loci_for_resistance_against_Verticillium_longisporum_in_oilseed_rape__Brassica_napus__ L2 - http://apsjournals.apsnet.org/doi/full/10.1094/PHYTO-98-2-0215?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -