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Analysis of expressed sequence tags from Uromyces appendiculatus hyphae and haustoria and their comparison to sequences from other rust fungi.
Phytopathology. 2008 Oct; 98(10):1126-35.P

Abstract

Hyphae, 2 to 8 days postinoculation (dpi), and haustoria, 5 dpi, were isolated from Uromyces appendiculatus infected bean leaves (Phaseolus vulgaris cv. Pinto 111) and a separate cDNA library prepared for each fungal preparation. Approximately 10,000 hyphae and 2,700 haustoria clones were sequenced from both the 5' and 3' ends. Assembly of all of the fungal sequences yielded 3,359 contigs and 927 singletons. The U. appendiculatus sequences were compared with sequence data for other rust fungi, Phakopsora pachyrhizi, Uromyces fabae, and Puccinia graminis. The U. appendiculatus haustoria library included a large number of genes with unknown cellular function; however, summation of sequences of known cellular function suggested that haustoria at 5 dpi had fewer transcripts linked to protein synthesis in favor of energy metabolism and nutrient uptake. In addition, open reading frames in the U. appendiculatus data set with an N-terminal signal peptide were identified and compared with other proteins putatively secreted from rust fungi. In this regard, a small family of putatively secreted RTP1-like proteins was identified in U. appendiculatus and P. graminis.

Authors+Show Affiliations

Soybean Genomics and Improvement Lab, USDA-ARS, Beltsville, MD 20705, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

18943459

Citation

Puthoff, D P., et al. "Analysis of Expressed Sequence Tags From Uromyces Appendiculatus Hyphae and Haustoria and Their Comparison to Sequences From Other Rust Fungi." Phytopathology, vol. 98, no. 10, 2008, pp. 1126-35.
Puthoff DP, Neelam A, Ehrenfried ML, et al. Analysis of expressed sequence tags from Uromyces appendiculatus hyphae and haustoria and their comparison to sequences from other rust fungi. Phytopathology. 2008;98(10):1126-35.
Puthoff, D. P., Neelam, A., Ehrenfried, M. L., Scheffler, B. E., Ballard, L., Song, Q., Campbell, K. B., Cooper, B., & Tucker, M. L. (2008). Analysis of expressed sequence tags from Uromyces appendiculatus hyphae and haustoria and their comparison to sequences from other rust fungi. Phytopathology, 98(10), 1126-35. https://doi.org/10.1094/PHYTO-98-10-1126
Puthoff DP, et al. Analysis of Expressed Sequence Tags From Uromyces Appendiculatus Hyphae and Haustoria and Their Comparison to Sequences From Other Rust Fungi. Phytopathology. 2008;98(10):1126-35. PubMed PMID: 18943459.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Analysis of expressed sequence tags from Uromyces appendiculatus hyphae and haustoria and their comparison to sequences from other rust fungi. AU - Puthoff,D P, AU - Neelam,A, AU - Ehrenfried,M L, AU - Scheffler,B E, AU - Ballard,L, AU - Song,Q, AU - Campbell,K B, AU - Cooper,B, AU - Tucker,M L, PY - 2008/10/24/pubmed PY - 2009/2/26/medline PY - 2008/10/24/entrez SP - 1126 EP - 35 JF - Phytopathology JO - Phytopathology VL - 98 IS - 10 N2 - Hyphae, 2 to 8 days postinoculation (dpi), and haustoria, 5 dpi, were isolated from Uromyces appendiculatus infected bean leaves (Phaseolus vulgaris cv. Pinto 111) and a separate cDNA library prepared for each fungal preparation. Approximately 10,000 hyphae and 2,700 haustoria clones were sequenced from both the 5' and 3' ends. Assembly of all of the fungal sequences yielded 3,359 contigs and 927 singletons. The U. appendiculatus sequences were compared with sequence data for other rust fungi, Phakopsora pachyrhizi, Uromyces fabae, and Puccinia graminis. The U. appendiculatus haustoria library included a large number of genes with unknown cellular function; however, summation of sequences of known cellular function suggested that haustoria at 5 dpi had fewer transcripts linked to protein synthesis in favor of energy metabolism and nutrient uptake. In addition, open reading frames in the U. appendiculatus data set with an N-terminal signal peptide were identified and compared with other proteins putatively secreted from rust fungi. In this regard, a small family of putatively secreted RTP1-like proteins was identified in U. appendiculatus and P. graminis. SN - 0031-949X UR - https://www.unboundmedicine.com/medline/citation/18943459/Analysis_of_expressed_sequence_tags_from_Uromyces_appendiculatus_hyphae_and_haustoria_and_their_comparison_to_sequences_from_other_rust_fungi_ L2 - https://apsjournals.apsnet.org/doi/10.1094/PHYTO-98-10-1126?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -