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In situ surface-enhanced Raman scattering analysis of biofilm.
Anal Chem. 2008 Nov 15; 80(22):8538-44.AC

Abstract

Biofilms represent the predominant form of microbial life on Earth. They are aggregates of microorganisms embedded in a matrix formed by extracellular polymeric substances (EPS). Detailed information about chemical composition and structure of the EPS matrix is relevant e.g. for the optimization of biocides, of antifouling strategies and for biological wastewater treatment. Raman microscopy (RM) is a capable tool that can provide detailed chemical information about biofilm constituents with spatial resolution of optical microscope. However, the sensitivity of RM is limited. Surface-enhanced Raman scattering (SERS), which enables investigations of biomolecules at very low concentration levels, allows overcoming this drawback. To our knowledge, this paper is the first report on reproducible SERS spectra from different constituents of a multispecies biofilm. We believe that the reproducibility is partly owed to the in situ measurement of the biofilm, while up to now SERS measurements of microbiological samples by RM were carried out after sample drying. We employed colloidal silver nanoparticles for in situ SERS measurements by RM. The achieved enhancement factor of up to 2 orders of magnitude illustrates a high potential of SERS for ultrasensitive chemical analysis of biofilms, including the detection of different components and the determination of their relative abundance in the complex biofilm matrix.

Authors+Show Affiliations

Institute of Hydrochemistry, Technische Universitat Munchen, Marchioninistrasse 17, D-81377, Munich, Germany. natalia.ivleva@ch.tum.deNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18947197

Citation

Ivleva, Natalia P., et al. "In Situ Surface-enhanced Raman Scattering Analysis of Biofilm." Analytical Chemistry, vol. 80, no. 22, 2008, pp. 8538-44.
Ivleva NP, Wagner M, Horn H, et al. In situ surface-enhanced Raman scattering analysis of biofilm. Anal Chem. 2008;80(22):8538-44.
Ivleva, N. P., Wagner, M., Horn, H., Niessner, R., & Haisch, C. (2008). In situ surface-enhanced Raman scattering analysis of biofilm. Analytical Chemistry, 80(22), 8538-44. https://doi.org/10.1021/ac801426m
Ivleva NP, et al. In Situ Surface-enhanced Raman Scattering Analysis of Biofilm. Anal Chem. 2008 Nov 15;80(22):8538-44. PubMed PMID: 18947197.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - In situ surface-enhanced Raman scattering analysis of biofilm. AU - Ivleva,Natalia P, AU - Wagner,Michael, AU - Horn,Harald, AU - Niessner,Reinhard, AU - Haisch,Christoph, Y1 - 2008/10/24/ PY - 2008/10/25/pubmed PY - 2009/2/3/medline PY - 2008/10/25/entrez SP - 8538 EP - 44 JF - Analytical chemistry JO - Anal Chem VL - 80 IS - 22 N2 - Biofilms represent the predominant form of microbial life on Earth. They are aggregates of microorganisms embedded in a matrix formed by extracellular polymeric substances (EPS). Detailed information about chemical composition and structure of the EPS matrix is relevant e.g. for the optimization of biocides, of antifouling strategies and for biological wastewater treatment. Raman microscopy (RM) is a capable tool that can provide detailed chemical information about biofilm constituents with spatial resolution of optical microscope. However, the sensitivity of RM is limited. Surface-enhanced Raman scattering (SERS), which enables investigations of biomolecules at very low concentration levels, allows overcoming this drawback. To our knowledge, this paper is the first report on reproducible SERS spectra from different constituents of a multispecies biofilm. We believe that the reproducibility is partly owed to the in situ measurement of the biofilm, while up to now SERS measurements of microbiological samples by RM were carried out after sample drying. We employed colloidal silver nanoparticles for in situ SERS measurements by RM. The achieved enhancement factor of up to 2 orders of magnitude illustrates a high potential of SERS for ultrasensitive chemical analysis of biofilms, including the detection of different components and the determination of their relative abundance in the complex biofilm matrix. SN - 1520-6882 UR - https://www.unboundmedicine.com/medline/citation/18947197/In_situ_surface_enhanced_Raman_scattering_analysis_of_biofilm_ L2 - https://doi.org/10.1021/ac801426m DB - PRIME DP - Unbound Medicine ER -