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Beneficial effects of ethyl pyruvate in a mouse model of spinal cord injury.
Shock. 2009 Aug; 32(2):217-27.S

Abstract

The aim of the present study was to evaluate in a mouse model of spinal cord injury (SCI) the effect of the treatment with ethyl pyruvate (EP). Spinal cord injury was induced by the application of vascular clips (force of 24 g) to the dura via a four-level T5-T8 laminectomy in mice. Treatment with EP (75, 25, or 8.5 mg/kg) 1 and 6 h after the SCI significantly decreased (a) the degree of spinal cord inflammation and tissue injury (histological score), (b) neutrophil infiltration (myeloperoxidase activity), (c) nitrotyrosine formation and iNOS expression, (d) proinflammatory cytokines expression, (e) nuclear factor kappaB activation, (f) extracellular signal-regulated kinase 1/2 mitogen-activated protein kinase phosphorylation, and (g) apoptosis (TUNEL staining, Fas ligand, Bax, and Bcl-2 expression). Moreover, EP (75, 25, or 8.5 mg/kg) significantly ameliorated in a dose-dependent manner the loss of limb function (evaluated by motor recovery score). Taken together, our results demonstrate that EP treatment reduces the development of inflammation and tissue injury associated with spinal cord trauma.

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Authors+Show Affiliations

Genovese T
IRCCS Centro Neurolesi Bonino-Pulejo, Messina, Italy.
Esposito E
No affiliation info available
Mazzon E
No affiliation info available
Di Paola R
No affiliation info available
Meli R
No affiliation info available
Caminiti R
No affiliation info available
Bramanti P
No affiliation info available
Fink MP
No affiliation info available
Cuzzocrea S
No affiliation info available

MeSH

AnimalsApoptosisDisease Models, AnimalDose-Response Relationship, DrugFas Ligand ProteinGene Expression RegulationHumansInflammationMaleMiceMitogen-Activated Protein Kinase 1Mitogen-Activated Protein Kinase 3Neutrophil InfiltrationNitric Oxide Synthase Type IIPeroxidasePhosphorylationPyruvatesSpinal Cord InjuriesTime FactorsTyrosinebcl-2-Associated X Protein

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18948848

Citation

Genovese, Tiziana, et al. "Beneficial Effects of Ethyl Pyruvate in a Mouse Model of Spinal Cord Injury." Shock (Augusta, Ga.), vol. 32, no. 2, 2009, pp. 217-27.
Genovese T, Esposito E, Mazzon E, et al. Beneficial effects of ethyl pyruvate in a mouse model of spinal cord injury. Shock. 2009;32(2):217-27.
Genovese, T., Esposito, E., Mazzon, E., Di Paola, R., Meli, R., Caminiti, R., Bramanti, P., Fink, M. P., & Cuzzocrea, S. (2009). Beneficial effects of ethyl pyruvate in a mouse model of spinal cord injury. Shock (Augusta, Ga.), 32(2), 217-27. https://doi.org/10.1097/SHK.0b013e31818d4073
Genovese T, et al. Beneficial Effects of Ethyl Pyruvate in a Mouse Model of Spinal Cord Injury. Shock. 2009;32(2):217-27. PubMed PMID: 18948848.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Beneficial effects of ethyl pyruvate in a mouse model of spinal cord injury. AU - Genovese,Tiziana, AU - Esposito,Emanuela, AU - Mazzon,Emanuela, AU - Di Paola,Rosanna, AU - Meli,Rosaria, AU - Caminiti,Rocco, AU - Bramanti,Placido, AU - Fink,Mitchell P, AU - Cuzzocrea,Salvatore, PY - 2008/10/25/pubmed PY - 2009/10/2/medline PY - 2008/10/25/entrez SP - 217 EP - 27 JF - Shock (Augusta, Ga.) JO - Shock VL - 32 IS - 2 N2 - The aim of the present study was to evaluate in a mouse model of spinal cord injury (SCI) the effect of the treatment with ethyl pyruvate (EP). Spinal cord injury was induced by the application of vascular clips (force of 24 g) to the dura via a four-level T5-T8 laminectomy in mice. Treatment with EP (75, 25, or 8.5 mg/kg) 1 and 6 h after the SCI significantly decreased (a) the degree of spinal cord inflammation and tissue injury (histological score), (b) neutrophil infiltration (myeloperoxidase activity), (c) nitrotyrosine formation and iNOS expression, (d) proinflammatory cytokines expression, (e) nuclear factor kappaB activation, (f) extracellular signal-regulated kinase 1/2 mitogen-activated protein kinase phosphorylation, and (g) apoptosis (TUNEL staining, Fas ligand, Bax, and Bcl-2 expression). Moreover, EP (75, 25, or 8.5 mg/kg) significantly ameliorated in a dose-dependent manner the loss of limb function (evaluated by motor recovery score). Taken together, our results demonstrate that EP treatment reduces the development of inflammation and tissue injury associated with spinal cord trauma. SN - 1540-0514 UR - https://www.unboundmedicine.com/medline/citation/18948848/Beneficial_effects_of_ethyl_pyruvate_in_a_mouse_model_of_spinal_cord_injury_ L2 - https://doi.org/10.1097/SHK.0b013e31818d4073 DB - PRIME DP - Unbound Medicine ER -