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Frequency of CCR5-Delta32 deletion in human immunodeficiency virus type 1 (HIV-1) in healthy blood donors, HIV-1-exposed seronegative and HIV-1-seropositive individuals of southern Brazilian population.
Int J Mol Med. 2008 Nov; 22(5):669-75.IJ

Abstract

The frequency of CCR5-Delta32 allele in human immunodeficiency virus type 1 (HIV-1) infection in the southern Brazilian population was determined in a cross-sectional study carried out from October 2001 to June 2004. Genomic DNA was extracted from peripheral blood cells of 134 healthy blood donors, 145 HIV-1-exposed seronegative individuals, 152 HIV-1-seropositive asymptomatic individuals, and 478 HIV-1-seropositive individuals with AIDS. A fragment with 225 base-pairs of the CCR5 gene was amplified by polymerase chain reaction. The CCR5-Delta32 homozygous deletion was observed in 2 (1.5%) blood donors and in 1 (0.7%) individual HIV-1-exposed seronegative, and was absent among all the HIV-1-seropositive individuals (Fisher's exact test, p=0.0242). The frequency of the homozygous CCR5-Delta32 deletion in the HIV-1-exposed did not differ when compared with that observed in the HIV-1 seronegative blood donors (Fisher's exact test, p=0.6093; OR: 2.18, 95% CI: 0.11-129.6). The wild-type genotype CCR5/CCR5 frequency was higher among the HIV-1-seropositive with AIDS compared to HIV-1 seropositive asymptomatic individuals (Chi-square test, p=0.0263; OR: 2.02, 95% CI: 1.03-3.97). The absence of the homozygous deletion of CCR5-Delta32 among HIV-1-seropositive individuals underscored that this genotype is an important genetic factor associated with the decreased susceptibility to HIV-1 infection. The higher frequency of heterozygosity for the CCR5-Delta32 and the CCR5-Delta32 allele in HIV-1 seropositive asymptomatic compared to HIV-seropositive with AIDS individuals also underscored that this deletion could be associated with the delay of the HIV-1 disease progression in this population. However, the low frequency of CCR5-Delta32 homozygosity observed among HIV-1-exposed seronegative individuals shows that the allele could not explain, by itself, the natural resistance to HIV-1 infection and different mechanisms of protection against HIV-1 infection that must be involved in this population.

Authors+Show Affiliations

Health Sciences Center, Department of Pathology, Clinical Analysis and Toxicology, University Hospital of Londrina State University, Vila Operária, CEP 86.038-440, Londrina, Paraná, Brazil. reiche@sercomtel.com.brNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Multicenter Study
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18949389

Citation

Reiche, Edna Maria Vissoci, et al. "Frequency of CCR5-Delta32 Deletion in Human Immunodeficiency Virus Type 1 (HIV-1) in Healthy Blood Donors, HIV-1-exposed Seronegative and HIV-1-seropositive Individuals of Southern Brazilian Population." International Journal of Molecular Medicine, vol. 22, no. 5, 2008, pp. 669-75.
Reiche EM, Ehara Watanabe MA, Bonametti AM, et al. Frequency of CCR5-Delta32 deletion in human immunodeficiency virus type 1 (HIV-1) in healthy blood donors, HIV-1-exposed seronegative and HIV-1-seropositive individuals of southern Brazilian population. Int J Mol Med. 2008;22(5):669-75.
Reiche, E. M., Ehara Watanabe, M. A., Bonametti, A. M., Morimoto, H. K., Akira Morimoto, A., Wiechmann, S. L., Matsuo, T., Carvalho De Oliveira, J., & Vissoci Reiche, F. (2008). Frequency of CCR5-Delta32 deletion in human immunodeficiency virus type 1 (HIV-1) in healthy blood donors, HIV-1-exposed seronegative and HIV-1-seropositive individuals of southern Brazilian population. International Journal of Molecular Medicine, 22(5), 669-75.
Reiche EM, et al. Frequency of CCR5-Delta32 Deletion in Human Immunodeficiency Virus Type 1 (HIV-1) in Healthy Blood Donors, HIV-1-exposed Seronegative and HIV-1-seropositive Individuals of Southern Brazilian Population. Int J Mol Med. 2008;22(5):669-75. PubMed PMID: 18949389.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Frequency of CCR5-Delta32 deletion in human immunodeficiency virus type 1 (HIV-1) in healthy blood donors, HIV-1-exposed seronegative and HIV-1-seropositive individuals of southern Brazilian population. AU - Reiche,Edna Maria Vissoci, AU - Ehara Watanabe,Maria Angelica, AU - Bonametti,Ana Maria, AU - Morimoto,Helena Kaminami, AU - Akira Morimoto,Arilson, AU - Wiechmann,Susana Lílian, AU - Matsuo,Tiemi, AU - Carvalho De Oliveira,Jaqueline, AU - Vissoci Reiche,Fernando, PY - 2008/10/25/pubmed PY - 2009/2/4/medline PY - 2008/10/25/entrez SP - 669 EP - 75 JF - International journal of molecular medicine JO - Int. J. Mol. Med. VL - 22 IS - 5 N2 - The frequency of CCR5-Delta32 allele in human immunodeficiency virus type 1 (HIV-1) infection in the southern Brazilian population was determined in a cross-sectional study carried out from October 2001 to June 2004. Genomic DNA was extracted from peripheral blood cells of 134 healthy blood donors, 145 HIV-1-exposed seronegative individuals, 152 HIV-1-seropositive asymptomatic individuals, and 478 HIV-1-seropositive individuals with AIDS. A fragment with 225 base-pairs of the CCR5 gene was amplified by polymerase chain reaction. The CCR5-Delta32 homozygous deletion was observed in 2 (1.5%) blood donors and in 1 (0.7%) individual HIV-1-exposed seronegative, and was absent among all the HIV-1-seropositive individuals (Fisher's exact test, p=0.0242). The frequency of the homozygous CCR5-Delta32 deletion in the HIV-1-exposed did not differ when compared with that observed in the HIV-1 seronegative blood donors (Fisher's exact test, p=0.6093; OR: 2.18, 95% CI: 0.11-129.6). The wild-type genotype CCR5/CCR5 frequency was higher among the HIV-1-seropositive with AIDS compared to HIV-1 seropositive asymptomatic individuals (Chi-square test, p=0.0263; OR: 2.02, 95% CI: 1.03-3.97). The absence of the homozygous deletion of CCR5-Delta32 among HIV-1-seropositive individuals underscored that this genotype is an important genetic factor associated with the decreased susceptibility to HIV-1 infection. The higher frequency of heterozygosity for the CCR5-Delta32 and the CCR5-Delta32 allele in HIV-1 seropositive asymptomatic compared to HIV-seropositive with AIDS individuals also underscored that this deletion could be associated with the delay of the HIV-1 disease progression in this population. However, the low frequency of CCR5-Delta32 homozygosity observed among HIV-1-exposed seronegative individuals shows that the allele could not explain, by itself, the natural resistance to HIV-1 infection and different mechanisms of protection against HIV-1 infection that must be involved in this population. SN - 1107-3756 UR - https://www.unboundmedicine.com/medline/citation/18949389/Frequency_of_CCR5_Delta32_deletion_in_human_immunodeficiency_virus_type_1__HIV_1__in_healthy_blood_donors_HIV_1_exposed_seronegative_and_HIV_1_seropositive_individuals_of_southern_Brazilian_population_ L2 - http://www.spandidos-publications.com/ijmm/22/5/669 DB - PRIME DP - Unbound Medicine ER -