Recognition mechanism of water-compatible molecularly imprinted solid-phase extraction and determination of nine quinolones in urine by high performance liquid chromatography.
J Chromatogr A. 2008 Nov 28; 1212(1-2):1-9.JC

Abstract

Novel water-compatible molecularly imprinted polymers (MIPs) were synthesized in methanol-water systems with ofloxacin as templates and methacryclic acid as functional monomers. The MIPs were used as a special sorbent for the selective solid-phase extraction (SPE) of nine quinolones from urine samples, showing high affinity to the quinolones in aqueous environment. Its molecular recognition mechanisms were investigated by the molecular simulation and the experimental validation with UV and infrared spectrogram as well as (1)H NMR. Binding capability and chromatographic characteristic were also evaluated. By using the water-compatible MIPs as SPE sorbents, the nine quinolones can be selectively extracted and enriched, while all matrices interferences were eliminated simultaneously. Under the optimal conditions of SPE and high performance liquid chromatography (HPLC), the good linearity of the method was obtained in a range of 0.05-30microg/mL with the correlation coefficient of >0.999 and the relative standard division of 2.0-7.4%. The detection limits (s/n=3) were in a range of 0.036-0.10microg/mL. The proposed method was successfully applied for the selective extraction and separation of the studied quinolones in urine samples.

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Authors+Show Affiliations

Sun HW

College of Chemical and Environmental Sciences, Hebei University, Key Laboratory of Analytical Science and Technology of Hebei Province, Baoding 071002, China. hanwei@hbu.edu.cn

Qiao FX

No affiliation info available

MeSH

Binding SitesChromatography, High Pressure LiquidComputer SimulationHumansHydrogen BondingHydrogen-Ion ConcentrationMethacrylatesModels, MolecularOfloxacinPolymersQuinolonesReproducibility of ResultsSensitivity and SpecificitySolid Phase ExtractionSolventsWater

Pub Type(s)

Evaluation Study

Journal Article

Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18950771

Citation

Sun, Han-wen, and Feng-xia Qiao. "Recognition Mechanism of Water-compatible Molecularly Imprinted Solid-phase Extraction and Determination of Nine Quinolones in Urine By High Performance Liquid Chromatography." Journal of Chromatography. A, vol. 1212, no. 1-2, 2008, pp. 1-9.

Sun HW, Qiao FX. Recognition mechanism of water-compatible molecularly imprinted solid-phase extraction and determination of nine quinolones in urine by high performance liquid chromatography. J Chromatogr A. 2008;1212(1-2):1-9.

Sun, H. W., & Qiao, F. X. (2008). Recognition mechanism of water-compatible molecularly imprinted solid-phase extraction and determination of nine quinolones in urine by high performance liquid chromatography. Journal of Chromatography. A, 1212(1-2), 1-9. https://doi.org/10.1016/j.chroma.2008.09.107

Sun HW, Qiao FX. Recognition Mechanism of Water-compatible Molecularly Imprinted Solid-phase Extraction and Determination of Nine Quinolones in Urine By High Performance Liquid Chromatography. J Chromatogr A. 2008 Nov 28;1212(1-2):1-9. PubMed PMID: 18950771.

* Article titles in AMA citation format should be in sentence-case

TY - JOUR T1 - Recognition mechanism of water-compatible molecularly imprinted solid-phase extraction and determination of nine quinolones in urine by high performance liquid chromatography. AU - Sun,Han-wen, AU - Qiao,Feng-xia, Y1 - 2008/10/07/ PY - 2008/04/24/received PY - 2008/09/17/revised PY - 2008/09/26/accepted PY - 2008/10/28/pubmed PY - 2009/2/4/medline PY - 2008/10/28/entrez SP - 1 EP - 9 JF - Journal of chromatography. A JO - J Chromatogr A VL - 1212 IS - 1-2 N2 - Novel water-compatible molecularly imprinted polymers (MIPs) were synthesized in methanol-water systems with ofloxacin as templates and methacryclic acid as functional monomers. The MIPs were used as a special sorbent for the selective solid-phase extraction (SPE) of nine quinolones from urine samples, showing high affinity to the quinolones in aqueous environment. Its molecular recognition mechanisms were investigated by the molecular simulation and the experimental validation with UV and infrared spectrogram as well as (1)H NMR. Binding capability and chromatographic characteristic were also evaluated. By using the water-compatible MIPs as SPE sorbents, the nine quinolones can be selectively extracted and enriched, while all matrices interferences were eliminated simultaneously. Under the optimal conditions of SPE and high performance liquid chromatography (HPLC), the good linearity of the method was obtained in a range of 0.05-30microg/mL with the correlation coefficient of >0.999 and the relative standard division of 2.0-7.4%. The detection limits (s/n=3) were in a range of 0.036-0.10microg/mL. The proposed method was successfully applied for the selective extraction and separation of the studied quinolones in urine samples. SN - 0021-9673 UR - https://www.unboundmedicine.com/medline/citation/18950771/Recognition_mechanism_of_water_compatible_molecularly_imprinted_solid_phase_extraction_and_determination_of_nine_quinolones_in_urine_by_high_performance_liquid_chromatography_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0021-9673(08)01679-8 DB - PRIME DP - Unbound Medicine ER -

Tags

Recognition mechanism of water-compatible molecularly imprinted solid-phase extraction and determination of nine quinolones in urine by high performance liquid chromatography.J Chromatogr A. 2008 Nov 28; 1212(1-2):1-9.JC