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Cofilin mediates tight-junction opening by redistributing actin and tight-junction proteins.
Biochem Biophys Res Commun. 2008 Dec 19; 377(3):921-5.BB

Abstract

We previously found that capsaicin induces tight-junction (TJ) opening accompanied with cofilin dephosphorylation/activation in intestinal Caco-2 cells. Here, we examined the role of cofilin in TJ regulation, and analyzed the structural events that lead to TJ opening. We transfected Caco-2 cells with wild-type cofilin [cofilin(wt)] or its constitutively active mutant cofilin(S3A). We found that the decreases in transepithelial electrical resistance (TER) was slower in cofilin(wt) transfectants and faster in cofilin(S3A) mutants than in vector controls. Moreover, cofilin dephosphorylation corresponded to the rate of TER decrease. Capsaicin treatment changed the localization of TJ proteins and altered the F-actin structure, but in a manner different from those depend on myosin light chain kinase (MLCK). These results strongly support the importance of cofilin in TJ opening, suggesting cofilin as a target for TJ permeability regulation in epithelial cells.

Authors+Show Affiliations

Graduate School of Life and Environmental Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki 305-8572, Japan. nag@agbi.tsukuba.ac.jpNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18952063

Citation

Nagumo, Yoko, et al. "Cofilin Mediates Tight-junction Opening By Redistributing Actin and Tight-junction Proteins." Biochemical and Biophysical Research Communications, vol. 377, no. 3, 2008, pp. 921-5.
Nagumo Y, Han J, Bellila A, et al. Cofilin mediates tight-junction opening by redistributing actin and tight-junction proteins. Biochem Biophys Res Commun. 2008;377(3):921-5.
Nagumo, Y., Han, J., Bellila, A., Isoda, H., & Tanaka, T. (2008). Cofilin mediates tight-junction opening by redistributing actin and tight-junction proteins. Biochemical and Biophysical Research Communications, 377(3), 921-5. https://doi.org/10.1016/j.bbrc.2008.10.071
Nagumo Y, et al. Cofilin Mediates Tight-junction Opening By Redistributing Actin and Tight-junction Proteins. Biochem Biophys Res Commun. 2008 Dec 19;377(3):921-5. PubMed PMID: 18952063.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Cofilin mediates tight-junction opening by redistributing actin and tight-junction proteins. AU - Nagumo,Yoko, AU - Han,Junkyu, AU - Bellila,Amor, AU - Isoda,Hiroko, AU - Tanaka,Toshiyuki, Y1 - 2008/10/24/ PY - 2008/10/04/received PY - 2008/10/17/accepted PY - 2008/10/28/pubmed PY - 2008/12/17/medline PY - 2008/10/28/entrez SP - 921 EP - 5 JF - Biochemical and biophysical research communications JO - Biochem Biophys Res Commun VL - 377 IS - 3 N2 - We previously found that capsaicin induces tight-junction (TJ) opening accompanied with cofilin dephosphorylation/activation in intestinal Caco-2 cells. Here, we examined the role of cofilin in TJ regulation, and analyzed the structural events that lead to TJ opening. We transfected Caco-2 cells with wild-type cofilin [cofilin(wt)] or its constitutively active mutant cofilin(S3A). We found that the decreases in transepithelial electrical resistance (TER) was slower in cofilin(wt) transfectants and faster in cofilin(S3A) mutants than in vector controls. Moreover, cofilin dephosphorylation corresponded to the rate of TER decrease. Capsaicin treatment changed the localization of TJ proteins and altered the F-actin structure, but in a manner different from those depend on myosin light chain kinase (MLCK). These results strongly support the importance of cofilin in TJ opening, suggesting cofilin as a target for TJ permeability regulation in epithelial cells. SN - 1090-2104 UR - https://www.unboundmedicine.com/medline/citation/18952063/Cofilin_mediates_tight_junction_opening_by_redistributing_actin_and_tight_junction_proteins_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0006-291X(08)02067-6 DB - PRIME DP - Unbound Medicine ER -