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A comparison of monocyte counts and ex vivo and in vitro monocyte cytokine production after major surgical trauma.
J Surg Res. 2009 Jun 01; 154(1):91-8.JS

Abstract

BACKGROUND

Impaired function of cluster of differentiation 14-positive (CD14+) monocytes (MOs) after major surgical trauma is believed to predispose to infectious complications. Postoperative decreases in human leukocyte antigen (HLA)-DR expression, tumor necrosis factor-alpha (TNF-alpha) production and interleukin (IL)-12 synthesis have been reported. There are no studies comparing absolute MO counts and MO cytokine synthesis in peripheral blood and stimulated cultures.

METHODS

The study group included 10 low-risk patients undergoing elective cardiac surgery with extracorporeal circulation. Preoperatively (d0) and on the first (d1) and third (d3) postoperative d, we analyzed leukocyte counts, CD14+ MO absolute counts, HLA-DR expression, and stimulated IL-12 and TNF-alpha synthesis using flow cytometry. In addition, IL-12 and TNF-alpha release in stimulated whole blood cultures was assayed.

RESULTS

Whereas the absolute numbers of leukocytes and CD14+ MOs were significantly elevated, HLA-DR expression was suppressed postoperatively. The proportion of TNF-alpha- and IL-12-producing MOs was reduced after surgery. This, however, led to a significant postoperative decrease only in the absolute numbers of peripheral blood IL-12+ MOs. IL-12 secretion was postoperatively reduced in whole blood cultures. The IL-12-synthesizing capacity of IL-12+ MOs was significantly reduced only on d1.

CONCLUSIONS

The immediate postoperative period is associated with an increase in the absolute MO numbers and an impairment of MO function, which is reflected in a reduced capacity to synthesize IL-12 and TNF-alpha and a decreased ability to express HLA-DR and present antigens. Whereas the cytokine-producing capacity returns to normal levels on d3, the suppression of HLA-DR expression persists.

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Authors+Show Affiliations

Franke A
Department of Trauma and Reconstructive Surgery, German Armed Forces Central Hospital, Koblenz, Germany.
Lante W
No affiliation info available
Kollig E
No affiliation info available
Markewitz A
No affiliation info available

MeSH

AgedCardiopulmonary BypassCell Culture TechniquesCytokinesElective Surgical ProceduresFemaleFlow CytometryHLA-DR AntigensHeart Valve Prosthesis ImplantationHemoglobinsHumansInterleukin-12Leukocyte CountLipopolysaccharide ReceptorsLipopolysaccharidesMaleMonocytesPostoperative PeriodTumor Necrosis Factor-alpha

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18952234

Citation

Franke, Axel, et al. "A Comparison of Monocyte Counts and Ex Vivo and in Vitro Monocyte Cytokine Production After Major Surgical Trauma." The Journal of Surgical Research, vol. 154, no. 1, 2009, pp. 91-8.
Franke A, Lante W, Kollig E, et al. A comparison of monocyte counts and ex vivo and in vitro monocyte cytokine production after major surgical trauma. J Surg Res. 2009;154(1):91-8.
Franke, A., Lante, W., Kollig, E., & Markewitz, A. (2009). A comparison of monocyte counts and ex vivo and in vitro monocyte cytokine production after major surgical trauma. The Journal of Surgical Research, 154(1), 91-8. https://doi.org/10.1016/j.jss.2008.06.003
Franke A, et al. A Comparison of Monocyte Counts and Ex Vivo and in Vitro Monocyte Cytokine Production After Major Surgical Trauma. J Surg Res. 2009 Jun 1;154(1):91-8. PubMed PMID: 18952234.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A comparison of monocyte counts and ex vivo and in vitro monocyte cytokine production after major surgical trauma. AU - Franke,Axel, AU - Lante,Wolfgang, AU - Kollig,Erwin, AU - Markewitz,Andreas, Y1 - 2008/07/11/ PY - 2008/02/26/received PY - 2008/04/30/revised PY - 2008/06/03/accepted PY - 2008/10/28/pubmed PY - 2009/7/9/medline PY - 2008/10/28/entrez SP - 91 EP - 8 JF - The Journal of surgical research JO - J Surg Res VL - 154 IS - 1 N2 - BACKGROUND: Impaired function of cluster of differentiation 14-positive (CD14+) monocytes (MOs) after major surgical trauma is believed to predispose to infectious complications. Postoperative decreases in human leukocyte antigen (HLA)-DR expression, tumor necrosis factor-alpha (TNF-alpha) production and interleukin (IL)-12 synthesis have been reported. There are no studies comparing absolute MO counts and MO cytokine synthesis in peripheral blood and stimulated cultures. METHODS: The study group included 10 low-risk patients undergoing elective cardiac surgery with extracorporeal circulation. Preoperatively (d0) and on the first (d1) and third (d3) postoperative d, we analyzed leukocyte counts, CD14+ MO absolute counts, HLA-DR expression, and stimulated IL-12 and TNF-alpha synthesis using flow cytometry. In addition, IL-12 and TNF-alpha release in stimulated whole blood cultures was assayed. RESULTS: Whereas the absolute numbers of leukocytes and CD14+ MOs were significantly elevated, HLA-DR expression was suppressed postoperatively. The proportion of TNF-alpha- and IL-12-producing MOs was reduced after surgery. This, however, led to a significant postoperative decrease only in the absolute numbers of peripheral blood IL-12+ MOs. IL-12 secretion was postoperatively reduced in whole blood cultures. The IL-12-synthesizing capacity of IL-12+ MOs was significantly reduced only on d1. CONCLUSIONS: The immediate postoperative period is associated with an increase in the absolute MO numbers and an impairment of MO function, which is reflected in a reduced capacity to synthesize IL-12 and TNF-alpha and a decreased ability to express HLA-DR and present antigens. Whereas the cytokine-producing capacity returns to normal levels on d3, the suppression of HLA-DR expression persists. SN - 1095-8673 UR - https://www.unboundmedicine.com/medline/citation/18952234/A_comparison_of_monocyte_counts_and_ex_vivo_and_in_vitro_monocyte_cytokine_production_after_major_surgical_trauma_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0022-4804(08)00397-1 DB - PRIME DP - Unbound Medicine ER -