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Protective effect of esculetin against oxidative stress-induced cell damage via scavenging reactive oxygen species.
Acta Pharmacol Sin. 2008 Nov; 29(11):1319-26.AP

Abstract

AIM

To investigate the anti-oxidant properties of esculetin (6,7-dihydroxycoumarin) against H2O2-induced Chinese hamster lung fibroblast (V79-4) damage.

METHODS

The radical scavenging activity was assessed by 1,1-diphenyl- 2-picrylhydrazyl (DPPH) radical, hydroxyl radical, and intracellular reactive oxygen species (ROS). In addition, lipid peroxidation was assayed by the measure of related substances which react with thiobarbituric acid. The amount of carbonyl formation in protein was determined using a protein carbonyl ELISA kit. As well, cellular DNA damage was detected by Western blot and immunofluorescence image. Cell viability was assessed by 3-(4,5-dimethylthiazole-2- yl)-2,5-diphenyltetrazolium bromide assay.

RESULTS

Esculetin exhibited DPPH radical scavenging, hydroxyl radical scavenging, and intracellular ROS scavenging activities. The radical scavenging activity of esculetin resulted in the protection of cells from lipid peroxidation, protein carbonyl, and DNA damage induced by H2O2. Therefore, esculetin recovered cell viability exposed to H2O2.

CONCLUSION

Esculetin efficiently attenuated the oxidative stress induced cell damage via its anti-oxidant properties. As a result, esculetin may be useful in the development of functional food and raw materials of medicine.

Authors+Show Affiliations

School of Medicine and Institute of Medical Science, Cheju National University, Jeju-si, Republic of Korea.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18954526

Citation

Kim, So-hyung, et al. "Protective Effect of Esculetin Against Oxidative Stress-induced Cell Damage Via Scavenging Reactive Oxygen Species." Acta Pharmacologica Sinica, vol. 29, no. 11, 2008, pp. 1319-26.
Kim SH, Kang KA, Zhang R, et al. Protective effect of esculetin against oxidative stress-induced cell damage via scavenging reactive oxygen species. Acta Pharmacol Sin. 2008;29(11):1319-26.
Kim, S. H., Kang, K. A., Zhang, R., Piao, M. J., Ko, D. O., Wang, Z. H., Chae, S. W., Kang, S. S., Lee, K. H., Kang, H. K., Kang, H. W., & Hyun, J. W. (2008). Protective effect of esculetin against oxidative stress-induced cell damage via scavenging reactive oxygen species. Acta Pharmacologica Sinica, 29(11), 1319-26. https://doi.org/10.1111/j.1745-7254.2008.00878.x
Kim SH, et al. Protective Effect of Esculetin Against Oxidative Stress-induced Cell Damage Via Scavenging Reactive Oxygen Species. Acta Pharmacol Sin. 2008;29(11):1319-26. PubMed PMID: 18954526.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Protective effect of esculetin against oxidative stress-induced cell damage via scavenging reactive oxygen species. AU - Kim,So-hyung, AU - Kang,Kyoung-ah, AU - Zhang,Rui, AU - Piao,Mei-jing, AU - Ko,Dong-ok, AU - Wang,Zhi-hong, AU - Chae,Sung-wook, AU - Kang,Sam-sik, AU - Lee,Keun-hwa, AU - Kang,Hee-kyoung, AU - Kang,Hyun-wook, AU - Hyun,Jin-won, PY - 2008/10/29/pubmed PY - 2009/5/1/medline PY - 2008/10/29/entrez SP - 1319 EP - 26 JF - Acta pharmacologica Sinica JO - Acta Pharmacol Sin VL - 29 IS - 11 N2 - AIM: To investigate the anti-oxidant properties of esculetin (6,7-dihydroxycoumarin) against H2O2-induced Chinese hamster lung fibroblast (V79-4) damage. METHODS: The radical scavenging activity was assessed by 1,1-diphenyl- 2-picrylhydrazyl (DPPH) radical, hydroxyl radical, and intracellular reactive oxygen species (ROS). In addition, lipid peroxidation was assayed by the measure of related substances which react with thiobarbituric acid. The amount of carbonyl formation in protein was determined using a protein carbonyl ELISA kit. As well, cellular DNA damage was detected by Western blot and immunofluorescence image. Cell viability was assessed by 3-(4,5-dimethylthiazole-2- yl)-2,5-diphenyltetrazolium bromide assay. RESULTS: Esculetin exhibited DPPH radical scavenging, hydroxyl radical scavenging, and intracellular ROS scavenging activities. The radical scavenging activity of esculetin resulted in the protection of cells from lipid peroxidation, protein carbonyl, and DNA damage induced by H2O2. Therefore, esculetin recovered cell viability exposed to H2O2. CONCLUSION: Esculetin efficiently attenuated the oxidative stress induced cell damage via its anti-oxidant properties. As a result, esculetin may be useful in the development of functional food and raw materials of medicine. SN - 1745-7254 UR - https://www.unboundmedicine.com/medline/citation/18954526/Protective_effect_of_esculetin_against_oxidative_stress_induced_cell_damage_via_scavenging_reactive_oxygen_species_ L2 - https://doi.org/10.1111/j.1745-7254.2008.00878.x DB - PRIME DP - Unbound Medicine ER -