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Susceptibility of Cry1Ab-resistant and -susceptible sugarcane borer (Lepidoptera: Crambidae) to four Bacillus thuringiensis toxins.
J Invertebr Pathol. 2009 Jan; 100(1):29-34.JI

Abstract

Sugarcane borer, Diatraea saccharalis (F.), is a primary corn stalk borer pest targeted by transgenic corn expressing Bacillus thuringiensis (Bt) proteins in many areas of the mid-southern region of the United States. Recently, genes encoding for Cry1A.105 and Cry2Ab2 Bt proteins were transferred into corn plants (event MON 89034) for controlling lepidopteran pests. This new generation of Bt corn with stacked-genes of Cry1A.105 and Cry2Ab2 will become commercially available in 2009. Susceptibility of Cry1Ab-susceptible and -resistant strains of D. saccharalis were evaluated on four selected Bt proteins including Cry1Aa, Cry1Ac, Cry1A.105, and Cry2Ab2. The Cry1Ab-resistant strain is capable of completing its larval development on commercial Cry1Ab-expressing corn plants. Neonates of D. saccharalis were assayed on a meridic diet containing one of the four Cry proteins. Larval mortality, body weight, and number of surviving larvae that did not gain significant weight (<0.1mg per larva) were recorded after 7 days. Cry1Aa was the most toxic protein against both insect strains, followed in decreasing potency by Cry1A.105, Cry1Ac, and Cry2Ab2. Using practical mortality (larvae either died or no significant weight gain after 7 days), the median lethal concentration (LC(50)) of the Cry1Ab-resistant strain was estimated to be >80-, 45-, 4.1-, and -0.5-fold greater than that of the susceptible strain to Cry1Aa, Cry1Ac, Cry1A.105 and Cry2Ab2 proteins, respectively. This information should be useful to support the commercialization of the new Bt corn event MON 89034 for managing D. saccharalis in the mid-southern region of the United States.

Authors+Show Affiliations

Department of Entomology, Louisiana State University Agricultural Center, Baton Rouge, LA 70803, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

18955062

Citation

Wu, Xiaoyi, et al. "Susceptibility of Cry1Ab-resistant and -susceptible Sugarcane Borer (Lepidoptera: Crambidae) to Four Bacillus Thuringiensis Toxins." Journal of Invertebrate Pathology, vol. 100, no. 1, 2009, pp. 29-34.
Wu X, Rogers Leonard B, Zhu YC, et al. Susceptibility of Cry1Ab-resistant and -susceptible sugarcane borer (Lepidoptera: Crambidae) to four Bacillus thuringiensis toxins. J Invertebr Pathol. 2009;100(1):29-34.
Wu, X., Rogers Leonard, B., Zhu, Y. C., Abel, C. A., Head, G. P., & Huang, F. (2009). Susceptibility of Cry1Ab-resistant and -susceptible sugarcane borer (Lepidoptera: Crambidae) to four Bacillus thuringiensis toxins. Journal of Invertebrate Pathology, 100(1), 29-34. https://doi.org/10.1016/j.jip.2008.10.003
Wu X, et al. Susceptibility of Cry1Ab-resistant and -susceptible Sugarcane Borer (Lepidoptera: Crambidae) to Four Bacillus Thuringiensis Toxins. J Invertebr Pathol. 2009;100(1):29-34. PubMed PMID: 18955062.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Susceptibility of Cry1Ab-resistant and -susceptible sugarcane borer (Lepidoptera: Crambidae) to four Bacillus thuringiensis toxins. AU - Wu,Xiaoyi, AU - Rogers Leonard,B, AU - Zhu,Yu Cheng, AU - Abel,Craig A, AU - Head,Graham P, AU - Huang,Fangneng, Y1 - 2008/10/14/ PY - 2008/08/04/received PY - 2008/09/30/revised PY - 2008/10/08/accepted PY - 2008/10/29/entrez PY - 2008/10/29/pubmed PY - 2009/2/7/medline SP - 29 EP - 34 JF - Journal of invertebrate pathology JO - J Invertebr Pathol VL - 100 IS - 1 N2 - Sugarcane borer, Diatraea saccharalis (F.), is a primary corn stalk borer pest targeted by transgenic corn expressing Bacillus thuringiensis (Bt) proteins in many areas of the mid-southern region of the United States. Recently, genes encoding for Cry1A.105 and Cry2Ab2 Bt proteins were transferred into corn plants (event MON 89034) for controlling lepidopteran pests. This new generation of Bt corn with stacked-genes of Cry1A.105 and Cry2Ab2 will become commercially available in 2009. Susceptibility of Cry1Ab-susceptible and -resistant strains of D. saccharalis were evaluated on four selected Bt proteins including Cry1Aa, Cry1Ac, Cry1A.105, and Cry2Ab2. The Cry1Ab-resistant strain is capable of completing its larval development on commercial Cry1Ab-expressing corn plants. Neonates of D. saccharalis were assayed on a meridic diet containing one of the four Cry proteins. Larval mortality, body weight, and number of surviving larvae that did not gain significant weight (<0.1mg per larva) were recorded after 7 days. Cry1Aa was the most toxic protein against both insect strains, followed in decreasing potency by Cry1A.105, Cry1Ac, and Cry2Ab2. Using practical mortality (larvae either died or no significant weight gain after 7 days), the median lethal concentration (LC(50)) of the Cry1Ab-resistant strain was estimated to be >80-, 45-, 4.1-, and -0.5-fold greater than that of the susceptible strain to Cry1Aa, Cry1Ac, Cry1A.105 and Cry2Ab2 proteins, respectively. This information should be useful to support the commercialization of the new Bt corn event MON 89034 for managing D. saccharalis in the mid-southern region of the United States. SN - 1096-0805 UR - https://www.unboundmedicine.com/medline/citation/18955062/Susceptibility_of_Cry1Ab_resistant_and__susceptible_sugarcane_borer__Lepidoptera:_Crambidae__to_four_Bacillus_thuringiensis_toxins_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0022-2011(08)00221-8 DB - PRIME DP - Unbound Medicine ER -